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A method of using tilapia biological control to stimulate Cryptocaryon infection

A technology that stimulates Cryptocaryon and biological control, applied in application, fish farming, climate change adaptation, etc., can solve problems such as outbreaks of infection, failure to prevent and control, stimulate Cryptocaryon strangulation, etc., to reduce infection , Prevention and control of secondary infection, environmental friendliness

Active Publication Date: 2022-03-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN106508754A discloses a biological control method that utilizes the filter-feeding ability of filter-feeding animals to remove stimulating Cryptocaryon ciliate larvae in aquaculture water, thereby controlling the density of ciliate larvae. Precursors, cysts and ciliated larvae, once the ciliated larvae are released from the cyst, the ciliated larvae will swim quickly in the water to find the infected host, causing an outbreak of infection, so this method cannot kill Cryptocaryon stimuli at an early stage , unable to achieve the dual role of prevention and control

Method used

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  • A method of using tilapia biological control to stimulate Cryptocaryon infection
  • A method of using tilapia biological control to stimulate Cryptocaryon infection
  • A method of using tilapia biological control to stimulate Cryptocaryon infection

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Sensitivity determination of red tilapia to the infection of Cryptocaryon irritant

[0038] 1. Experimental method

[0039] To evaluate the sensitivity of red tilapia to Cryptocaryon stimuli infection, red tilapia (3.94±0.53g) were artificially infected with Cryptocaryon stimuli, and compared with pompano ovata (13.07±2.37g). A total of 3 groups of experiments were set up:

[0040] The first group was 30 pomfrets raised alone as the control group 1;

[0041] The second group is 6 red tilapias raised alone, as the control group 2;

[0042] The third group is the polyculture of pomfret and red tilapia, including 30 pomfret and 6 tilapia.

[0043] Each group of 3 parallel, were temporarily raised in 9 water tanks. Each water tank was filled with 100 liters of seawater, and 3000 Cryptocaryon stimuli larvae were added within 2 hours of incubation, and infected for 2 hours; then the fish of the 3 groups after infection were transferred to clean water tanks. On ...

Embodiment 2

[0046] Example 2 Determination of the effect of red tilapia feeding on stimulation of Cryptocaryon cyst

[0047] 1. Experimental method

[0048] (1) Determination of cyst consumption rate of red tilapia feeding stimulation Cryptocaryonia

[0049] Cyst precursors were collected from Pompano ovata heavily infested with Cryptocaryon irritant using glass plates with a diameter of 20 cm. Count the collected cyst precursors (N 1 ), the range of the number of cysts is controlled at 96-1475 / plate. Then the plate was transferred to a 120L circular water tank, and 50L seawater was added to the water tank, and one red tilapia (4.45±1.06g) was added to each water tank, and oxygen exposure was continued. Let the red tilapia ingest the cysts within 5 hours, finally take out the plate, and count the number of remaining cysts (N 2 ), calculate the consumption rate of red tilapia to cyst: cyst consumption rate=(N 1 -N 2 ) / 5.

[0050] (2) Determination of the degree of infection of pomfr...

Embodiment 3

[0057] Example 3 Using red tilapia polyculture to carry out biological control of Cryptocaryon stimuli

[0058] 1. Experimental method

[0059] 1. Seawater domestication of red tilapia

[0060] Taking red tilapia as an example, 50 red tilapias (4.46±0.78g, bred for 2 weeks) are temporarily raised in each breeding tank, and the breeding density is 100 fish / m 3 ;The water temperature is 26-32°C, continuous oxygen exposure, static water culture; transfer the red tilapia to seawater with a salinity of 12‰ for 2 days, and then increase the seawater salinity to 24‰ at a rate of 6‰ / 2d, Finally, increase the seawater salinity to 28‰. Feed 3% of the fish body weight every day, 1.5% each time, and feed twice a day (8:00, 16:00).

[0061] 2. Polyculture experiment of red tilapia and pomfret

[0062] Taking pomfret ovata as the infection object, the indoor simulation experiment was carried out. The specific experimental process is as follows:

[0063] (1) Temporary culture of experim...

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Abstract

The invention provides a method for stimulating Cryptocaryon infection by biological control of tilapia, comprising the following steps: S1. domesticating tilapia in seawater; S2. Polyculture of fish with nuclei. In the present invention, red tilapia and pomfret are polycultured, the polyculture ratio is 3:20, and the number of tilapias is 19 / m 2 , can significantly reduce the insect load of pomfret ovata and improve its survival rate; it can effectively prevent and control the secondary infection of fish susceptible to Cryptocaryon stimuli, and can prevent and control Cryptokaryon stimuli It is an environmentally friendly, green and safe biological control method, and it is worth promoting in seawater fish farming.

Description

technical field [0001] The invention relates to the technical field of fish disease prevention and control, in particular to a method for stimulating Cryptocaryon infection by biological control of tilapia. Background technique [0002] Cryptocaryonium irritatus is a protozoan ciliate that parasitizes the body of fish. It can infect almost all cultured bony fish in southern my country, such as pomfret, large yellow croaker, and grouper. Severe infection can cause Cryptocaryonia irritatis disease , also known as "white spot disease", this disease is currently the most serious parasitic disease in marine fish farming, which can cause hundreds of millions of economic losses every year. [0003] In recent years, scholars at home and abroad have carried out a lot of research work on the control of Cryptocaryon stimuli, including immune control, drug control, ecological control and so on. Immunization prevention and control includes the research and development of vaccines and imm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/13
CPCA01K61/13Y02A40/81
Inventor 李安兴钟志鸿
Owner SUN YAT SEN UNIV
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