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Application of regulating EIF4A3 expression to regulate and control proliferation capacity of hepatoma carcinoma cells

A liver cancer cell, proliferation ability technology, applied in the field of bioinformatics, can solve problems such as unclear role

Pending Publication Date: 2021-06-25
叶甲舟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the role of EIF4A3 in the proliferation of HCC cells remains unclear

Method used

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  • Application of regulating EIF4A3 expression to regulate and control proliferation capacity of hepatoma carcinoma cells
  • Application of regulating EIF4A3 expression to regulate and control proliferation capacity of hepatoma carcinoma cells
  • Application of regulating EIF4A3 expression to regulate and control proliferation capacity of hepatoma carcinoma cells

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Embodiment 1

[0069] like figure 1 As shown, the present invention provides a flowchart of the molecular mechanism and application of regulating the expression of EIF4A3 to affect the proliferation of liver cancer cells. Four types of liver cancer data were obtained from the TCGA database. After differential expression analysis, genes related to EIF4A3 expression were identified, and modules related to liver cancer were explored through weighted gene co-expression network analysis. After functional enrichment analysis, EIF4A3 was constructed to participate in liver cancer cells. An integrated regulatory network for proliferation.

[0070] like figure 1 As shown, the present invention provides a method for constructing a comprehensive regulatory network that regulates the expression of EIF4A3 to affect the proliferation of liver cancer cells, comprising the following steps:

[0071] (1) Data processing:

[0072] Download liver cancer data from the Cancer Genome Atlas (TCGA) database to obta...

Embodiment 2

[0091] (1) Construction of a cell line that knocks out EIF4A3:

[0092] Prepare recombinant EIF4A3-shRNA lentivirus and negative control (NC) lentivirus; infect LM3 cells with a lentiviral vector (EIF4A3-KD) that knocks out the EIF4A3 gene, and obtain stably infected knockout EIF4A3 cell lines and control cell lines. like Figure 5 A shows the transfection efficiency after knocking out EIF4A3, indicating that the liver cancer cell line LM3 has indeed knocked out EIF4A3. The specific process is as follows:

[0093] qRT-PCR was used for detection, and the sequencing results were compared with the sequence of EIF4A3. Log in to the Public TRCPortal website, search for EIF4A3 and select the verified and high-scoring shRNA interference sequence 5′-GGATGAAGCTGATGAAATG-3′ of EIF4A3 from the results, and compare it by BLAST homology. The synthetic shRNA template (nucleotide sequence as shown in SED ID NO: 1, specifically GGATGAAGCTGATGAAATG; the specific synthesis process is aimed a...

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Abstract

The invention relates to an application of regulating EIF4A3 expression to regulate and control liver cancer cell proliferation ability, which analyzes the effect of EIF4A3 in liver cancer and explores the pathway and function of EIF4A3 related gene enrichment through bioinformatics, and then predicts and constructs a comprehensive regulation and control network of EIF4A3 participating in liver cancer cell proliferation. On the basis, liver cancer cell lines LM3 and Hep3B are used as sources to construct a cell line interfering EIF4A3 expression, the influence of interference of EIF4A3 expression on liver cancer cell proliferation is further clarified, a new insight is provided for the effect in liver cancer progression, a potential treatment target of the disease is determined, and a reliable theoretical basis is provided for application of EIF4A3 in genetic engineering, oncology and clinical diagnosis indexes and treatment targets of liver cancer.

Description

technical field [0001] The invention relates to the technical fields of bioinformatics and genetic engineering, discloses an application of regulating the expression of EIF4A3 to regulate the proliferation ability of liver cancer cells, and specifically relates to a molecular mechanism and application of regulating the expression of EIF4A3 to affect the proliferation of liver cancer cells. Background technique [0002] Primary liver cancer is the second leading cause of cancer-related death worldwide. Primary liver cancer includes hepatocellular carcinoma (HCC, hereinafter referred to as liver cancer), intrahepatic cholangiocarcinoma (ICCA) and other rare tumors (Sia D, Villanueva A, Friedman SL, et al. Liver Cancer Cell of Origin, Molecular Class, and Effects on Patient Prognosis [J]. Gastroenterology, 2016, 152(4): 745.). Liver cancer is the digestive system tumor with the highest mortality rate in my country. Early treatment of liver cancer is extremely important. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858G16B30/00G16B40/00
CPCC12Q1/6886C12Q1/6858G16B30/00G16B40/00C12Q2600/158C12Q2600/156C12Q2539/103
Inventor 叶甲舟梁嵘林燕张锦燕骆敏马良
Owner 叶甲舟