Method for improving tissue culture germination rate of strawberry seeds

Abstract

The invention discloses a method for improving the tissue culture germination rate of strawberry seeds, and belongs to the field of tissue culture of wild strawberry seeds. The method for improving the tissue culture germination rate of the strawberry seeds comprises the steps of seed soaking, culture medium and appliance preparation, seed and appliance disinfection, seed secondary sterilization, seed coat treatment and seed inoculation. According to the method for improving the tissue culture germination rate of the strawberry seeds, the tissue culture germination rate of the wild strawberry seeds can be increased to 70% or above from 0, the high germination rate is achieved, the natural germination rate of the strawberry seeds is only 30-40%, and the natural germination rate of the strawberry seeds is far exceeded through the method; by sterilizing and disinfecting the seeds, the growth environment of the seeds can be effectively ensured, meanwhile, the tissue of the wild strawberry seeds is awakened in the sterilization process, and the physiological dormancy of the seeds is broken by treating seed coats with a physical method, so that the germination rate of the strawberry seeds is greatly increased.

Description

technical field [0001] The invention relates to the field of tissue culture of wild strawberry seeds, in particular to a method for improving the tissue culture germination rate of strawberry seeds. Background technique [0002] Strawberry belongs to the Rosaceae Fragaria genus, is a perennial evergreen herb plant, and belongs to the berry fruit tree in horticulture. Strawberry fruit is tender and juicy, sweet and sour, with unique flavor and rich nutrition. Compared with other fruit trees, the cultivation period is short and suitable for facility cultivation. It is a fruit tree with high economic benefits. The continuous improvement and renewal of strawberry varieties is the basis for the development of the strawberry industry in the world. So far, the strawberry varieties that have been bred at home and abroad are mainly obtained through conventional cross-breeding methods, and the seedlings obtained from hybrid seeds are selected for breeding. Sowing seeds and obtaining ...