Low-cost rapid micropropagation method for excellent eucommia ulmoides seedlings

A low-cost and excellent technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of seasonal and time constraints, long breeding cycle, etc., to shorten the breeding cycle, vigorous growth state, Easy-to-Master Effects

Active Publication Date: 2021-07-09
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to solve the problem that the existing eucommia breeding method has a long breeding cycle and the breeding is limited by seasons and time

Method used

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  • Low-cost rapid micropropagation method for excellent eucommia ulmoides seedlings
  • Low-cost rapid micropropagation method for excellent eucommia ulmoides seedlings
  • Low-cost rapid micropropagation method for excellent eucommia ulmoides seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0042] A low-cost and rapid micropropagation method for excellent seedlings of Eucommia ulmoides, comprising the following steps:

[0043] (1) Take materials

[0044] From the beginning of April to the beginning of May, select the perennial Eucommia ulmoides with excellent agronomic traits (one or more of high yield, large leaves, fast growth, and strong stress resistance) in the field, with one leaf and one bud, which are 2.0-3.0cm long. The robust micro-stem segment is an explant;

[0045] (2) Pretreatment of explants

[0046] Rinse the stem section in step (1) with running water, soak it in low-temperature plasma-activated water for 2 minutes, and take it out for later use;

[0047] (3) Induction treatment of explant adventitious roots

[0048] Place the pretreated stem section base in step (2) in an adventitious root-inducing solution for 30 s, wherein the adventitious root-inducing solution contains sodium nitroprusside and 800 μM melatonin with a final concentration o...

Embodiment 2

[0055] A low-cost and rapid micropropagation method for excellent seedlings of Eucommia ulmoides, comprising the following steps:

[0056] (1) Take materials

[0057] From the first ten days of May to the first ten days of June, the perennial Eucommia ulmoides with excellent agronomic traits (one or more of high yield, large leaves, fast growth, and strong stress resistance) in the field were selected, with one leaf and one bud. The 3.0cm robust micro-stem segment is the explant;

[0058] (2) Pretreatment of explants

[0059] Rinse the stem section in step (1) with running water, soak it in low-temperature plasma-activated water for 4 minutes, and take it out for later use;

[0060] (3) Induction treatment of explant adventitious roots

[0061] Place the base of the sterilized stem segment in step (2) in an adventitious root-inducing solution for 30 s, wherein the adventitious root-inducing solution is sodium nitroprusside and 900 μM melatonin containing a final concentrati...

Embodiment 3

[0069] A low-cost and rapid micropropagation method for excellent seedlings of Eucommia ulmoides, comprising the following steps:

[0070] (1) Take materials

[0071] From the end of June to the end of July, the field perennial Eucommia ulmoides with excellent agronomic traits (one or more of high yield, large leaves, fast growth, and strong resistance to stress) was selected in the current year with one leaf and one bud. The 3.0cm robust micro-stem segment is the explant;

[0072] (2) Pretreatment of explants

[0073] Rinse the stem section in step (1) with running water, soak it in low-temperature plasma-activated water for 6 minutes, and take it out for later use;

[0074] (3) Induction treatment of explant adventitious roots

[0075] Place the base of the sterilized stem segment in step (2) in an adventitious root-inducing solution for 30 s, wherein the adventitious root-inducing solution contains sodium nitroprusside and 1000 μM melatonin at a final concentration of 50...

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Abstract

The invention discloses a low-cost rapid micropropagation method for excellent eucommia ulmoides seedlings. The method comprises the following steps: by taking a micro-stem segment with one leaf and one bud of an eucommia ulmoides strain as an explant, performing pretreatment, performing adventitious root induction treatment, performing field planting of the explant, performing adventitious root induction culture, performing axillary bud germination induction of the explant, performing seedling culture and the like. A rooting inducer is adopted to treat the stem segments for 30 s, adventitious root primordia are formed in 7-10 d, complete eucommia ulmoides regenerated plants can be obtained in 22 d, the speed is higher than that of rapid propagation of eucommia ulmoides through a traditional propagation method and a tissue culture technology, and the propagation period is greatly shortened; and according to the method, the micro-stem segments are inserted into a seedling raising plate filled with a nutrient medium and directly take roots and seedlings in a greenhouse, on one hand, the influence and limitation of natural factors such as seasons and regions are avoided, and on the other hand, the defects that the tissue culture and breeding process of eucommia ulmoides is tedious and the domestication and transplanting survival rate of subsequent tissue culture regeneration seedlings is low are overcome.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, and more specifically relates to a low-cost and rapid micropropagation method for fine Eucommia seedlings. Background technique [0002] Eucommia ulmoides Oliver (Eucommia ulmoides Oliver), also known as Sizhong and Silk Mianmu, belongs to Eucommiaceae. Eucommia is a perennial woody plant. It is a unique economic plant and medicinal plant in my country. living fossil plants. Eucommia ulmoides has strong adaptability, can resist cold, drought, salt and alkali, and pests and diseases. It is an ideal tree species for urban and rural greening, and plays an important role in watershed management, soil and water conservation, greening of barren hills, passages and urban street greening. [0003] Eucommia ulmoides, as a unique medicinal material in my country, has the functions of "three lowering" (lowering blood pressure, lowering blood sugar, lowering blood fat), "four protecting"...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 侯金艳吴丽芳王大成苏鹏飞王萍丁双双
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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