Seedling strengthening and rapid propagation method for polygonatum cyrtonema

A multi-flower Polygonatum, a robust technology, applied in the field of plant tissue culture, can solve the problems of limiting the yield potential of Polygonatum, inconvenient planting management and promotion, large amount of rhizomes, etc., achieve good market and industrial application prospects, high reproduction efficiency, and simple operation Effect

Active Publication Date: 2021-07-13
安徽省林业高科技开发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the current artificial cultivation, the propagation mainly depends on the vegetative propagation of rhizomes. This method has low reproduction efficiency and a large amount of rhizomes.

Method used

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  • Seedling strengthening and rapid propagation method for polygonatum cyrtonema
  • Seedling strengthening and rapid propagation method for polygonatum cyrtonema
  • Seedling strengthening and rapid propagation method for polygonatum cyrtonema

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of strong seedling rapid propagation method of Polygonatum polyflora of the present embodiment, comprises the steps:

[0040] (1) Aseptic system construction:

[0041] Select Polygonatum multiflora tubers with bud eyes, wash off the mud and dirt on the surface of the material, soak them in washing powder water for 10 minutes, then lightly brush the buds one by one with a fine brush, and rinse them under running water for 1 hour; then, take them to the For the ultra-clean workbench, soak it in 75% alcohol for 8 seconds, rinse it twice with sterile water, then soak it in 0.1% sodium hypochlorite solution for 15 minutes, rinse it with sterile water six times, and absorb it with high-pressure sterilized filter paper. surface moisture of dry tubers;

[0042] Cut the above-mentioned sterilized Polygonatum polyflora tubers into small pieces of 3cm*5cm size with one or two cryptobuds on the operating table, and inoculate into pre-designed starting medium (MS+6.5g / L agar...

Embodiment 2

[0052] A kind of strong seedling rapid propagation method of Polygonatum polyflora of the present embodiment, comprises the steps:

[0053] (1) Aseptic system construction:

[0054] Select Polygonatum multiflora tubers with bud eyes, wash off the mud and dirt on the surface of the material, soak them in washing powder water for 15 minutes, then lightly brush the buds one by one with a fine brush, and rinse them under running water for 3 hours; then, take them to the For the ultra-clean workbench, soak it in 75% alcohol for 10 seconds, rinse it with sterile water for 4 times, then soak it in 0.1% sodium hypochlorite solution for 25 minutes, rinse it with sterile water for 8 times, and absorb it with high-pressure sterilized filter paper. surface moisture of dry tubers;

[0055] Cut the above-mentioned sterilized Polygonatum polyflora tubers into 3cm*5cm sized pieces with one or two cryptobuds on the operating table, and inoculate into pre-designed starting medium (MS+7.5g / L ag...

Embodiment 3

[0065] A kind of strong seedling rapid propagation method of Polygonatum polyflora of the present embodiment, comprises the steps:

[0066] (1) Aseptic system construction:

[0067] Select Polygonatum multiflora tubers with bud eyes, wash off the mud and dirt on the surface of the material, soak them in washing powder water for 12 minutes, then lightly brush the buds one by one with a fine brush, and rinse them under running water for 2 hours; then, take them to the For the ultra-clean workbench, soak it in 75% alcohol for 9 seconds, rinse it with sterile water for 3 times, then soak it in 0.1% sodium hypochlorite solution for 20 minutes, rinse it with sterile water for 7 times, and absorb it with high-pressure sterilized filter paper. surface moisture of dry tubers;

[0068] Cut the above-mentioned sterilized Polygonatum polyflora tubers into small pieces of 3cm*5cm size with one or two cryptobuds on the operating table, and inoculate into pre-designed starting medium (MS+7....

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Abstract

The invention provides a seedling strengthening and rapid propagation method for polygonatum cyrtonema, which comprises the following steps: (1) cutting polygonatum cyrtonema cluster buds into blocks, inoculating a proliferation, seedling strengthening and propagation medium with the blocks for subculture, and carrying out repeated cutting and subculture for multiple times to obtain robust cluster buds; (2) cutting the robust cluster buds into bud tubers with adventitious buds, and inoculating a rooting culture medium with the bud tubers for rooting culture; (3) putting rooted test-tube plantlets in a culture room for hardening under light, taking out the rooted test-tube plantlets from a culture bottle, and washing off the culture medium at the roots of the rooted test-tube plantlets; then, soaking the rooted test-tube plantlets with carbendazim, and after soaking is completed, planting the rooted test-tube plantlets into matrix mesh bags, and watering the rooted test-tube plantlets sufficiently with root fixing water; and finally, culturing the rooted test-tube plantlets in a greenhouse. According to the method, the polygonatum cyrtonema buds are subjected to induced differentiation to obtain the cluster buds, the operation is simple, the culture cost is low, the propagation efficiency is high, tissue culture industrialized production can be achieved, and the method is an important way for excellent germplasm preservation and seedling batch production of polygonatum cyrtonema.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for strong and rapid propagation of Polygonatum polyflora. Background technique [0002] Polygonatum multiflora is a perennial herb of Polygonatum genus of Liliaceae, which has medicinal, edible, ornamental and beauty value. Polygonatum multiflora has delicate meat and rhizomes similar to ginger. It has the functions of invigorating the spleen and nourishing qi, nourishing the lungs and kidneys, nourishing yin and promoting body fluid, etc. It has been regarded as a precious Chinese medicinal material by the Chinese medicine community since ancient times, and has the title of "the king of blood and qi". reputation. [0003] Polygonatum multiflora is mainly distributed in Sichuan, Guizhou, Anhui, Jiangsu, Zhejiang and other places in China, and often grows in scattered, sporadic or small flakes in damp deciduous broad-leaved forests, forest margins, mountain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 许早时汪耀朱谦孙刚孟可张贤萍孔伶俐姚瑶
Owner 安徽省林业高科技开发中心
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