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A humanized mouse model of cytomegalovirus infection and its construction method

A technology of cytomegalovirus and construction method, which is applied to the humanized mouse model of cytomegalovirus infection and its construction field, can solve the problems of limited dose, poor stability, and cannot guarantee the survival of mice, and achieves reduced modeling The effect of short cycle, planting and living cycle, and speeding up the progress of experimental research

Active Publication Date: 2022-07-12
PEOPLES HOSPITAL PEKING UNIV
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Problems solved by technology

Therefore, the method has poor stability, is limited by dosage and cannot guarantee the survival of mice

Method used

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  • A humanized mouse model of cytomegalovirus infection and its construction method
  • A humanized mouse model of cytomegalovirus infection and its construction method
  • A humanized mouse model of cytomegalovirus infection and its construction method

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Embodiment 1

[0050] Take 6-8 week old NSG mice and irradiate 150cGy with RS2000 X-ray biological irradiator. In the 8th hour after irradiation, 1×10 peripheral stem cells mobilized by G-CSF were reinfused through the tail vein of mice. 6 Peripheral stem cells were derived from healthy donors with positive serum HCMV IgG. On the 15th day, 100ul of mouse orbital vein peripheral blood was collected, and flow cytometry was used to detect the proportion of hCD45+ cells in mouse peripheral blood. like figure 2 If the proportion of hCD45+ cells detected is greater than 2%, the MRC-5 cells infected with AD169 (MOI=2) were intraperitoneally injected with 1×10 6 / Only.

[0051] Finally, mice were observed within two weeks after intraperitoneal injection of AD169-infected MRC-5 cells. It was observed that after intraperitoneal injection of AD169-infected MRC-5 cells, mice did not develop adverse symptoms such as acute GVHD and death caused by acute GVHD, and, such as image 3 As shown, on the 15...

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Abstract

The present invention provides a humanized mouse model of cytomegalovirus infection and a construction method thereof. The construction method includes: constructing an initial model by infusing G-CSF mobilized human peripheral stem cells into NSG mice; wherein, the The input of G‑CSF mobilized human peripheral stem cells was 1×10 6 per mouse; after the human peripheral stem cells are engrafted, cytomegalovirus is injected into the initial model to obtain a stable cytomegalovirus-infected humanized mouse model. The construction method provided by the present invention can reduce the occurrence of GVHD, and provide a stable place for experimental research based on the model in the later stage; meanwhile, the engraftment period of human peripheral stem cells is short, which greatly shortens the modeling period and speeds up the later stage. Based on the experimental research progress of the model, the modeling method provided by the present invention has broad application prospects.

Description

technical field [0001] The invention belongs to the field of genetic engineering and genetic modification, in particular to a humanized mouse model infected with cytomegalovirus and a construction method thereof. Background technique [0002] Cytomegalovirus (Cytomegalovirus, abbreviated as CMV) belongs to the Beta-herpesvirus family and is the largest and most complex virus in the human herpes family. Under normal circumstances, CMV is widely spread in the population, and the positive rate of infection in some areas can reach 90%. After the first infection, it can be latent for life. Activation of CMV is the leading cause of death in immunocompromised patients. [0003] Mice have become the first choice for animal models of human diseases because of their high homology to the human genome, many inbred lines, short reproductive cycles, and easy feeding. However, due to the strict species specificity of CMV, previous studies have mainly used murine cytomegalovirus (MCMV) in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/0271A61K49/0008A01K2227/105A01K2267/0337Y02A50/30
Inventor 黄晓军赵翔宇余星星商倩楠
Owner PEOPLES HOSPITAL PEKING UNIV
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