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Premature coronary heart disease-related genes and their detection reagents and applications

A coronary heart disease and gene technology, applied in the field of early-onset coronary heart disease-related genes and their detection reagents, can solve the problems of being susceptible to factors such as drugs and mental conditions, demanding detection time, and low sensitivity and specificity. The effect of reducing the number of children born and promoting the development of innovative drugs

Active Publication Date: 2022-02-18
百世诺(北京)医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have many disadvantages, such as many types of examinations, low sensitivity and specificity, easily affected by factors such as drugs and mental conditions; the detection time is demanding, etc.

Method used

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  • Premature coronary heart disease-related genes and their detection reagents and applications
  • Premature coronary heart disease-related genes and their detection reagents and applications
  • Premature coronary heart disease-related genes and their detection reagents and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, LDLR gene C.834DELG heterozygous deletion mutation gene, in vitro detection of LDLR gene C.834DELG heterozygous missing mutant genes

[0031] This example provides an LDLR gene C.834DELG heterozygous deletion mutation gene, which is shown in SEQ ID NO: 1. The mutant primary gene sequence is GaatctActG gtctgacct g tcccagagaa tgatctgcag. Deleted mutation gene sequences such as SEQ ID NO: 1: GaatctActG gtctgacct TCCCAGAA TGATCTGCAG. This embodiment also provides forward primers for detecting LDLR gene C.834DELG hybrid deletion mutation genes, as shown in SEQ ID NO: 2, reverse primers such as SEQ ID NO: 3.

[0032] SEQ ID NO: 2: 5'-aggcactcttggttccatcgac-3 ';

[0033] SEQ ID NO: 3: 5'-agtccacagccagccccgtc-3 '.

[0034] An in vitro detection of LDLR gene C.834DELG heterozygous deletion mutation gene is provided in an inverted mutant gene of the present invention, the kit comprising: 1) amplification LDLR gene C.834DELG heterozygous deletion mutation gene of primer; 2)...

Embodiment 2

[0037] Example 2, in vitro detecting whether there is a method of coronary heart disease related genes in the sample

[0038] The method of present in vitro whether there is a germorant related gene in the presence of an early coronary heart disease in the sample, including the following steps:

[0039] 1. PCR amplification is performed on the C.834DELG site of the sample to be tested; wherein the sample to be tested is blood, hair, saliva, hair or biomass to be tested.

[0040] Among them, DNA extraction of samples to be tested using a DNA extraction of the sample kit (Bai Nuo) using a magnetic bead method: Add 200 μL sample (serum / whole blood) to 96 deep hole plates 1, 7, 10μL The protease K, the pipette is blown mixed, and 150 μl of the binding fluid is added after 10 to 15 minutes at room temperature. Add 96 deep hole plates into the fully automatic nucleic acid extraction purifier ZK-01, start DNA extraction, and the program is set to Table 2:

[0041] Table 2

[0042]

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Abstract

The invention discloses a premature coronary heart disease-related gene and its detection reagent and application, a method for in vitro detecting whether there is a coronary heart disease-related gene in a sample to be tested, 1) extracting the DNA of the sample to be tested, targeting the c of the LDLR gene .834delG site for PCR amplification; 2) analyzing the PCR amplification product; 3) identifying whether the c.834delG site of the LDLR gene is missing. According to the present invention, by detecting whether the LDLR gene c.834delG is a heterozygous deletion mutation, patients with premature coronary heart disease can be distinguished from normal people. Therefore, this variation can be used as a biomarker for clinical auxiliary diagnosis of premature coronary heart disease; Whether the subject carries the above-mentioned mutation can detect the carrier of the mutation, provide eugenic guidance and genetic counseling for the subject, and reduce the birth of children; provide possible drug treatment targets for humans to overcome premature coronary heart disease, and promote innovation Drug Discovery.

Description

Technical field [0001] The present invention relates to the field of biological engineering, and more particularly to genes related genes related to early coronary heart disease and their detection reagents and applications. Background technique [0002] PRMATURE CORONARY ARTERY DISEASE, PCAD is a special form of coronary heart disease. According to the 3rd meeting report of the Adult Treatment Group of the US National Cholesterol Education (NECPIII) stipulates that early coronary heart disease is the incidence of coronary heart disease Age male <55 years old, female <65 years old. Studies at home and abroad have shown that PCAD is in a young group in a young population with a low ratio. There is no sign before the onset of PCAD, and the incidence is inhabited. It is mostly acute coronary syndrome, high mortality; due to its special age, it will bring heavy economic burdens to society and families. For the traditional authentication and diagnosis methods of PCAD, it mainly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12Q1/6883C12Q1/6858C12N15/11
CPCC07K14/705C12Q1/6883C12Q1/6858C12Q2600/156
Inventor 刘哲李九翔张陆明侯青梁庆渊刘锋赵娜娜刘昕超惠汝太
Owner 百世诺(北京)医疗科技有限公司
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