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A compound microecological preparation for preventing and treating poultry necrotizing enteritis and its application

A technology of compound microecology and necrotizing enteritis, applied in the field of feed, can solve the problem of Bacillus velesi

Active Publication Date: 2022-08-09
TIANJIN CHIATAI FEED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Lactobacillus plantarum, Lactobacillus salivarius, Bifidobacterium, etc. have been reported to have antibacterial activity against Clostridium perfringens. enteritis reports

Method used

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  • A compound microecological preparation for preventing and treating poultry necrotizing enteritis and its application
  • A compound microecological preparation for preventing and treating poultry necrotizing enteritis and its application
  • A compound microecological preparation for preventing and treating poultry necrotizing enteritis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Screening and identification of antagonistic species of Clostridium perfringens, Salmonella and Escherichia coli

[0039] 1 Materials method

[0040] 1.1 Test material

[0041] 1.1.1 Bacteria

[0042] Clostridium perfringens, Salmonella enterica subsp. enterica and Escherichia coli were purchased from China Industrial Microorganism Culture Collection Center, and probiotic strains were isolated and preserved by Beijing Keweibo Biotechnology Research Institute.

[0043] 1.1.2 Culture medium

[0044] (1) Tryptone-sulfite medium

[0045] Tryptone 15.0g, soybean peptone 5.0g, yeast powder 5.0g, sodium metabisulfite 1.0g, ferric ammonium citrate 1.0g, pH 7.6±0.2, sterilized for 30min.

[0046] Solid medium: add 1.6% agar powder on the basis of liquid medium.

[0047] (2) LB medium

[0048] Tryptone 10.0g, yeast extract 5.0g, sodium chloride 10.0g, add distilled water to 1000mL, pH7.0±0.1, 121℃, sterilize for 30min.

[0049] Solid medium: add 1.6% agar powder on...

Embodiment 2

[0079] The preparation of embodiment 2 compound probiotics

[0080] 1. The production method steps of Bacillus velesi are as follows:

[0081] (1) inoculate the Bacillus velesi strain on the slant medium, and cultivate at 30-40 ° C for 12-24 hours;

[0082] (2) inoculate the Bacillus velesi cultured on the slant in the seed activated liquid medium, and cultivate for 12-24 hours under the conditions of 30-40° C. and 160-220 rpm;

[0083] (3) inoculate the Bacillus velesi seed solution in the seed tank at a weight ratio of 0.5%-2%, and cultivate for 12-24 hours under the conditions of 30-40 ° C and 160-220 rpm;

[0084] (4) The seed tank culture solution is inoculated in the fermenter with a weight ratio of 0.5%-2%, the tank pressure is 0.01-0.05MPa, the stirring speed is 160-220rpm, and the ventilation ratio is 0.5-1:0.1 in terms of volume ratio -0.5, fermentation time is 16-24 hours;

[0085] (5) The fermentation broth of Bacillus velesi is spray-dried through a drying towe...

Embodiment 3

[0098] Example 3 Effects of compound probiotics on growth performance and intestinal flora of broilers

[0099] 1 Test material

[0100] Bacillus velesi, Lactobacillus plantarum and compound microecological preparations were produced and provided by Beijing Keweibo Biotechnology Co., Ltd., and Clostridium perfringens was purchased from China Industrial Microorganism Culture Collection Center.

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Abstract

The invention discloses a composite microecological preparation for preventing and treating poultry necrotizing enteritis and its application, which are compounded with Bacillus velesi, Lactobacillus plantarum and xylo-oligosaccharide as active ingredients. The Bacillus velesi and Lactobacillus plantarum strains in the present invention have a strong inhibitory effect on intestinal pathogenic bacteria such as Clostridium perfringens, Salmonella and Escherichia coli, and combined with the intestinal probiotic function of xylo-oligosaccharide, compound use can Regulate the intestinal health of poultry, effectively prevent and cure poultry necrotizing enteritis, and reduce the use of antibiotics in the breeding process.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to the preparation and application method of a composite microecological preparation for preventing and treating poultry enteritis. Background technique [0002] Poultry necrotizing enteritis is a serious intestinal disease with acute onset, high mortality, and huge economic losses to poultry breeding. The prevention and treatment of necrotizing enteritis in poultry production is very important. [0003] Clostridium perfringens is the main pathogenic factor that causes necrotizing enteritis in poultry. It is a strict anaerobic bacterium, Gram-positive, Clostridium stubby under the microscope, with blunt ends at both ends, which can form spores , spores are mesogenic or proximal, oval in shape. Clostridium perfringens is a resident bacteria in the intestinal tract of poultry and is a typical opportunistic pathogen, which does not cause necrotizing enteritis in poultry under normal cond...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/75A23K10/18A23K20/163A61K35/747A61P1/00
CPCA23K50/75A23K10/18A23K20/163A61K35/742A61K35/747A61P1/00A23V2400/169A61K2300/00Y02A40/70
Inventor 李富伟吴培均韩明渠
Owner TIANJIN CHIATAI FEED TECH
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