Traditional Chinese medicine composition as well as preparation method and application thereof in preventing or resisting diarrhea
A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese veterinary medicine, can solve the problems of easy generation of drug resistance, food safety and public health, etc.
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Embodiment 1
[0052] The preparation of embodiment 1 Chinese medicine compound
[0053] Recipe: Patrinia 80 parts, Qinpi 80 parts, Brucea javanica 20 parts, Chen AI 60 parts, Cardamom 60 parts, Atractylodes macrocephala 60 parts, Burnet 50 parts, Nepeta charcoal 60 parts, Sargassum 80 parts.
[0054] The preparation method comprises the following steps:
[0055] (1) Take the above medicinal materials and screen and clean them respectively according to the ratio of parts by weight;
[0056] (2) Fructus Bruceae is pulverized to 60 order, adds the 75vt% ethanol solution immersion extraction of 4 times of quality for 24 hours, collects extract, reclaims ethanol, obtains Fructus Bruceae extract, for subsequent use;
[0057] (3) Grinding other medicinal materials to 20-30 mesh; using dynamic countercurrent extraction method for extraction, the extraction solvent is drinking water, the mass ratio of solvent to raw material is 10:1, the heating temperature is 80°C, and the extraction time is 2 hou...
Embodiment 2
[0061] 1. Materials and methods
[0062] 1.1. Experimental animals
[0063] From March 2020 to January 2021, 362 cattle with diarrhea were selected from Luhuo County, Daofu County, Baiyu County, Kangding City, and Litang County, Ganzi Prefecture. The clinical symptoms of bovine diarrhea are shown in Table 1. There are differences in clinical symptoms. Most yaks with diarrhea have loss of appetite, are thin, and unresponsive, and some have panting, shortness of breath or dyspnea. More stools are yellow or yellow-green.
[0064] Table 1 Clinical symptoms of bovine diarrhea
[0065]
[0066] 1.2. Experimental drugs
[0067] Commonly used antibiotics: enrofloxacin, gentamicin; Chinese medicine compound prepared in Example 1 and normal saline.
[0068] 1.3. Test method
[0069] 362 cows with diarrhea symptoms were selected and divided into Chinese medicine group, positive medicine group and control group.
[0070] Chinese medicine group: the Chinese medicine compound prep...
Embodiment 3
[0084] 1. Materials and methods
[0085] From April 2021 to May 2021, 100 calves with diarrhea were selected from a dairy farm in Anhui. The clinical symptoms of calf diarrhea are shown in Table 3. There are differences in clinical symptoms. Most calves with diarrhea have loss of appetite, listlessness, and some have runny nose. Most of the feces are yellow or yellow-green, and the shape is watery and pasty.
[0086] Table 3 Clinical symptoms of calf diarrhea in dairy cows
[0087]
[0088] Note: Dairy farms classify calf diarrhea into grades 1-5. Grade 1 is mild diarrhea, Grade 2 is more severe, Grade 3 is severe diarrhea, Grade 4 is mild dehydration and diarrhea, Grade 5 is severe dehydration and diarrhea with blood in the stool. Generally, manual treatment is required for grade 2 in the factory, so calves with grade 2-3 diarrhea are selected for testing.
[0089] 2. Experimental method
[0090] A total of 100 10-day-old calves with diarrhea were selected in this expe...
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