Walnut meal anti-inflammatory peptide WPL and application thereof

A technology for anti-inflammatory peptides and anti-inflammatory drugs is applied in the field of anti-inflammatory peptides isolated from enzymatic hydrolysis products of walnut meal, and can solve the problems of expensive purification method chromatography technology, increased operating costs, and high purification costs.
CN113201047AActive Publication Date: 2021-08-03BEIJING FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2021-08-03

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Abstract

The invention belongs to the technical field of food deep processing, and particularly relates to an anti-inflammatory peptide separated from walnut meal enzymolysis products and application of the anti-inflammatory peptide. The amino acid sequence of the anti-inflammatory peptide from the C terminal to the N terminal is Trp-Pro-Leu, WPL for short. The preparation method comprises the following steps: preparing anti-inflammatory polypeptide from walnut meal through enzymolysis, purifying by adopting ultrafiltration and sephadex chromatography, identifying a polypeptide sequence through LC-MS/MS, screening anti-inflammatory activity fragments by utilizing a molecular docking technology, artificially synthesizing the polypeptide with relatively high score, verifying the anti-inflammatory activity of the polypeptide, and finding that the WPL has the anti-inflammatory activity. The compound has different degrees of inhibition rates on the generation amounts of three inflammatory media (NO, TNF-[alpha] and IL-1[beta]). Molecular docking results show that WPL and iNOS are connected mainly through [pi]-[pi] accumulation, alkyl bonds, [pi]-alkyl bonds and hydrogen bonds.
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Description

Technical field:

[0001] The invention belongs to the technical field of food deep processing, and in particular relates to an anti-inflammatory peptide isolated from an enzymatic hydrolysis product of walnut dregs and an application thereof. Background technique:

[0002] In recent years, various chronic diseases caused by chronic inflammation have seriously threatened human health. Inflammation inducers trigger the production of various inflammatory mediators through various sensors present in immune cells, and then trigger inflammatory responses through several signaling pathways. Inflammatory mediators refer to chemical substances released by immune cells or produced by body fluids that can cause inflammatory reactions during inflammation. They can be divided into seven categories according to their biochemical characteristics: vasoactive amines, vasoactive peptides, lipid mediators, and complement components. fragments, cytokines, chemokines and proteolytic enzymes (Medz...

Claims

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