Walnut meal anti-inflammatory peptide WSL and application thereof

A technology for anti-inflammatory peptides and anti-inflammatory drugs is applied in the field of anti-inflammatory peptides isolated from enzymatic hydrolysis products of walnut meal, and can solve the problems of expensive purification method chromatography technology, increased operating costs, and high purification costs.
CN113307843AActive Publication Date: 2021-08-27BEIJING FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2021-08-27

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Abstract

The invention belongs to the technical field of food deep processing, and particularly relates to an anti-inflammatory peptide separated from walnut meal enzymolysis products and application of the anti-inflammatory peptide. The amino acid sequence of the anti-inflammatory peptide from the C terminal to the N terminal is Trp-Ser-Leu, WSL for short. The anti-inflammatory polypeptide is prepared from walnut meal through enzymolysis, purifying is carried out by adopting ultrafiltration and sephadex chromatography, the polypeptide sequence is identified through LC-MS / MS, anti-inflammatory activity fragments are screened by utilizing a molecular docking technology, the polypeptide with relatively high score is subjected to artificial synthesis, the anti-inflammatory activity of the polypeptide is verified, and the invention finds that the WSL has the anti-inflammatory activity, and has different degrees of inhibition rates on the generation amounts of four inflammatory mediators (NO, TNF-alpha, IL-6 and IL-1beta). Molecular docking results show that the WSL and iNOS are connected mainly through pi-pi accumulation, alkyl bonds, pi-alkyl bonds and hydrogen bonds.
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Description

Technical field:

[0001] The invention belongs to the technical field of food deep processing, and in particular relates to an anti-inflammatory peptide isolated from an enzymatic hydrolysis product of walnut dregs and an application thereof. Background technique:

[0002] In recent years, various chronic diseases caused by chronic inflammation have seriously threatened human health. Inflammation inducers trigger the production of various inflammatory mediators through various sensors present in immune cells, and then trigger inflammatory responses through several signaling pathways. Inflammatory mediators refer to chemical substances released by immune cells or produced by body fluids that can cause inflammatory reactions during inflammation. They can be divided into seven categories according to their biochemical characteristics: vasoactive amines, vasoactive peptides, lipid mediators, and complement components. fragments, cytokines, chemokines and proteolytic enzymes (Medz...

Claims

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