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Culture medium and method for inducing plant to form storage roots

A medium and basic medium technology, applied in the fields of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of not obtaining cassava roots, not forming secondary structures, etc.

Pending Publication Date: 2021-09-03
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous methods only accumulated starch in test-tube seedling roots, did not form secondary structures, and failed to obtain the process of cassava roots from expanding to real storage root morphology

Method used

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  • Culture medium and method for inducing plant to form storage roots
  • Culture medium and method for inducing plant to form storage roots
  • Culture medium and method for inducing plant to form storage roots

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Experimental program
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preparation example Construction

[0132] The present invention also provides a method for preparing a plant medium, comprising mixing basal medium, 6-BA, NAA, sucrose and optional jasmonic acid compounds, the plant medium contains 0.05mg / L-0.2mg / L of 6-BA, 10-100 μg / L NAA, 2-6% sucrose, 1 mg / L-4 mg / L jasmonic acid compound, the jasmonic acid compound is jasmonic acid or its derivatives. The plant medium is used to induce plants to form storage roots. The plant is preferably selected from the group consisting of: sweet potato, cassava, Polygonum multiflorum, Dahlia, Asparagus, Poncea.

[0133] The present invention also provides a plant culture method for inducing a plant to form a storage root, comprising culturing a plant or part of a plant with the plant medium described herein. Plants having storage roots can be obtained using the cultivation methods herein. "Culture" as used herein includes various types of plant culture, such as tissue culture, plant culture, pot culture, and field culture. The tissue c...

Embodiment 1

[0160] Embodiment 1, the cassava root induced by different sucrose concentrations

[0161] The result of 2% sucrose concentration as figure 1 As shown, the primary root has a tendency to expand, but the root maintains the primary structure. There is no accumulation of starch granules in the root.

[0162] The result of sucrose concentration of 4% is as follows figure 2 As shown, the trend of root expansion has been very obvious, and the number of fibrous roots has increased. There is a large amount of starch accumulation in the roots of the plants (stained as dark parts), but the primary structure is still maintained.

[0163] The result of 5% sucrose concentration as image 3 As shown, the expansion of roots was inhibited compared with 4% sucrose treatment, and the number of wild-type fibrous roots increased. Section results showed that starch accumulation in roots disappeared.

Embodiment 2

[0164] Embodiment 2, the cassava root induced by different concentrations of NAA

[0165] The results of NAA concentration of 0.5μg / L are as follows Figure 4 As shown, the root has a tendency to become thicker, and the section results show that starch has accumulated in the root (stained as a dark part).

[0166] The results of NAA concentration of 10μg / L are as follows Figure 5 , the number of fibrous roots increased, the roots of the plant became thicker obviously, and there was starch accumulation (dark part stained).

[0167] The results of NAA concentration of 50 μg / L are as follows Image 6, there was an obvious swelling phenotype, and a small amount of starch accumulated in the swelling root, but it was still a primary root structure.

[0168] The results of NAA concentration of 100μg / L are as follows Figure 7 , the roots are all thickened or swollen, with a small amount of starch accumulation (stained as a dark part), but the cell structure of the root is loose....

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Abstract

The invention provides a plant culture medium which comprises a basic culture medium, 0.05-0.2 mg / L of 6-BA, optional NAA, optional sucrose and optional jasmonic acid compounds. The jasmonic acid compounds are jasmonic acid or derivatives thereof. The plant culture medium is used to form plant storage roots. The invention also provides a method for inducing a plant to form the storage roots.

Description

technical field [0001] The invention belongs to the field of plant cultivation, in particular to a culture medium and a method for inducing plants to form storage roots. Background technique [0002] Storage root is an important organ of root crops, and its growth and development status directly determines the yield and productivity of crops. However, so far, little is known about the development of storage roots in root crops. The main difficulty of the research is that the storage root is the underground organ of the plant, which is not conducive to direct and continuous observation; and the field environment is unstable, and the research is greatly disturbed by environmental factors. [0003] As an important method of preserving plant germplasm, test-tube seedling culture has the advantages of no pollution, stable environment, and easy observation. Inducing the formation of storage roots of test-tube plantlets is not only beneficial to the research on the development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张鹏吴晓运张博伦
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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