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Hericium erinaceus mycelium polysaccharide with effect of preventing and treating senile dementia and preparation method thereof

A mycelium polysaccharide and senile dementia technology, applied in the field of medicine, can solve the problems of polysaccharide biological activity, polysaccharide molecular breakage, time and power polysaccharide results, etc., to slow down the symptoms of senile dementia and alleviate the apoptosis of nerve cells , Improve the effect of relevant biochemical indicators in the brain

Active Publication Date: 2021-09-03
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction method in this patent is ultrasonic-assisted, because time and power have a great influence on the polysaccharide results, which may cause the polysaccharide molecules to break and thus affect the biological activity of the obtained polysaccharides

Method used

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  • Hericium erinaceus mycelium polysaccharide with effect of preventing and treating senile dementia and preparation method thereof
  • Hericium erinaceus mycelium polysaccharide with effect of preventing and treating senile dementia and preparation method thereof
  • Hericium erinaceus mycelium polysaccharide with effect of preventing and treating senile dementia and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1. The Hericium erinaceus mycelium obtained by submerged liquid fermentation is ultrafinely pulverized into powder after freeze-drying, and passed through a 150-mesh sieve;

[0039] 2. Add deionized water 12 times the mass of the powder, stir evenly, soak for 20 minutes, and extract in a constant temperature water bath at 80°C for 2.5 hours;

[0040] 3. After cooling to room temperature, centrifuge to take the supernatant, concentrate to 1 / 10 volume by rotary evaporation, and remove protein by Sevag method;

[0041]4. Concentrate the supernatant, use 40% (v / v) ethanol for precipitation, 4°C overnight, collect the precipitate, and freeze-dry to obtain the crude polysaccharide of Hericium erinaceus mycelium (HE-1).

Embodiment 2

[0043] 1. The Hericium erinaceus mycelium obtained by submerged liquid fermentation is ultrafinely pulverized into powder after freeze-drying, and passed through a 150-mesh sieve;

[0044] 2. Add 10 times the mass of deionized water, stir well, soak for 30 minutes, and extract in a constant temperature water bath at 75°C for 2.5 hours;

[0045] 3. After cooling to room temperature, centrifuge to take the supernatant, concentrate to 1 / 10 volume by rotary evaporation, and remove protein by Sevag method;

[0046] 4. Concentrate the supernatant, use 60% (v / v) ethanol for precipitation, and spend overnight at 4° C., collect the precipitate, and freeze-dry to obtain the crude polysaccharide of Hericium erinaceus mycelium (HE-2).

Embodiment 3

[0048] 1. The Hericium erinaceus mycelium obtained by submerged liquid fermentation is ultrafinely pulverized into powder after freeze-drying, and passed through a 150-mesh sieve;

[0049] 2. Add 12 times the mass of deionized water, stir evenly, soak for 30 minutes, and extract in a constant temperature water bath at 80°C for 2.5 hours;

[0050] 3. After cooling to room temperature, centrifuge to take the supernatant, concentrate to 1 / 6 volume by rotary evaporation, and remove protein by Sevag method;

[0051] 4. Concentrate the supernatant, use 80% (v / v) ethanol for precipitation, and overnight at 4°C, collect the precipitate, and freeze-dry to obtain the crude polysaccharide of Hericium erinaceus mycelium (HE-3).

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Abstract

A hericium erinaceus mycelium polysaccharide with the effect of preventing and treating senile dementia and a preparation method are disclosed. Hericium erinaceus mycelium obtained through a liquid submerged fermentation technology serves as a raw material, the neuroprotective effect serves as an evaluation standard, and the novel purified hericium erinaceus mycelium polysaccharide is obtained through extraction, separation and purification, and is composed of six monosaccharides including fucose, galactose, xylose, glucose, mannose and glucuronic acid; and experiments prove that in APP / PS1 mice, the hericium erinaceus mycelium purified polysaccharide obtained in the invention can obviously shorten platform searching time and movement distance of the mice, improve platform jumping staying time of the mice, and reduce platform jumping-off times, and has effects of improving the cognition and memory ability of APP / PS1 mice, effectively reducing the levels of Abeta1-42, Tau (phospo S396) and 4-HNE in hippocampal regions of the mice, relieving AD pathological symptoms and improving the cholinergic system and the antioxidant ability.

Description

technical field [0001] The invention discloses a Hericium erinaceus mycelium polysaccharide with the effect of preventing and treating senile dementia. field of medical technology. Background technique [0002] Alzheimer's disease (AD) causes memory and other cognitive decline and is responsible for more than 60 percent of dementia cases. Worldwide, more than 50 million people with dementia are diagnosed with AD, and this number is projected to increase to nearly 152 million by 2050. The pathogenesis of AD is still unclear, and there are many factors affecting AD, including age, gender, living environment, genetic background and so on. More and more studies have shown that the occurrence of AD is not a unilateral effect of one factor, but a multi-faceted synergistic effect that leads to cognitive impairment. Alkaliergic injury hypothesis, neuroinflammation hypothesis, gene mutation hypothesis, Ca 2+ Disequilibrium theory, etc. It is difficult to develop drugs targeting ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P25/28A61P25/00A61K36/07
CPCC08B37/0003A61K31/715A61P25/28A61P25/00A61K36/07A61K2236/19A61K2236/17A61K2236/15A61K2236/331
Inventor 王迪孔繁格胡文继官月李玉刘洋苏玲李兰州
Owner JILIN AGRICULTURAL UNIV
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