Nucleic acid aptamer QQ-2 targeting human highly invasive choroidal melanoma and application

Abstract

The invention relates to a nucleic acid aptamer QQ-2 targeting human highly invasive choroidal melanoma and application. By identifying the property characterization and target molecules of the nucleic acid aptamer QQ-2, a tumor marker of the choroidal melanoma can be found, and the nucleic acid aptamer QQ-2 is used for diagnosis and treatment and targeted treatment of the choroidal melanoma. The prepared nucleic acid aptamer can specifically recognize human highly invasive choroidal melanoma cells and choroidal melanoma tissues and has high affinity; the nucleic acid aptamer has no immunogenicity; the molecular weight is small, and in-vitro chemical synthesis can be achieved; and different parts of the nucleic acid aptamer are easy to modify and replace. Meanwhile, the nucleic acid aptamer has the advantages of being stable in sequence, convenient to store, convenient to mark and the like. When the nucleic acid aptamer is adopted for detecting the human choroidal melanoma cells, operation is easier and faster, the synthesis cost of the nucleic acid aptamer is lower than the cost of antibody preparation, the period is short, and reproducibility is good.

Description

technical field [0001] The invention specifically relates to the technical field of molecular biology, in particular to a nucleic acid aptamer QQ-2 targeting human highly invasive choroidal melanoma and its application. Background technique [0002] Uveal melanoma is the most common primary malignant intraocular tumor in adults and the second most common type of melanoma after the skin. Tumors originated in the uvea, including the choroid (90%), ciliary body (6%), and iris (4%). Uveal melanoma is usually asymptomatic and has no specific tumor serum markers. Diagnosis is mainly based on ophthalmic imaging techniques, including fundus photography, fluorescein angiography, fundus autofluorescence imaging, optical coherence tomography, and ultrasound imaging, which also makes Uveal melanoma has emerged as one of the few cancers not primarily diagnosed by biopsy. [0003] Uveal melanoma is prone to metastasis, especially liver metastasis. Multiple studies have found that more ...

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Problems solved by technology

However, the use of antibodies as molecular probes often lacks ideal pharmacokineti

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