Collection, separation and culture integrated device for clinical endometrial stem cells

Abstract

The invention discloses a collection, separation and culture integrated device for clinical endometrial stem cells. The collection, separation and culture integrated device comprises a separation console, a semiconductor refrigerator, a first motor, a first centrifugal chamber, a first filter vat, a first collecting tank, a second motor, a second centrifugal chamber, a second filter vat, a first liquid storage tank, a third motor, a third centrifugal chamber, a third filter vat, a second collecting tank, a fourth motor, a fourth centrifugal chamber, a fourth filter vat, a second liquid storage tank, a first suction pump, a connecting hose, a collecting handle, a micro motor, a shaft rod, a rotary cutting sheet, a culture sleeve, a heater and a culture tank. By virtue of the collecting device, the endometrium collection amount and the cell viability can be improved, the pollution probability in the collection process is greatly reduced, and by means of the integrated structure, operation is convenient, electronic automation is achieved, and quantitative regulation and control are achieved; and in addition, by means of a multi-group separation culture mode, the migration ability of the cells can be enhanced, so that the cells have multidirectional differentiation potential, and finally the purpose of in-vitro rapid amplification is achieved.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to an integrated device for collection, isolation and culture of endometrial stem cells in clinical practice. Background technique [0002] In recent years, researchers have discovered that menstrual blood stem cells can form special heart cells that may one day be used to treat and repair weakened or damaged hearts. Research data shows that the number of stem cells obtained from menstrual blood is about 30 times that of bone marrow, which can be used for the treatment of various diseases after culture, such as premature ovarian failure, muscular dystrophy, heart disease, etc. Therefore, menstrual blood may become the largest in the world. source of stem cells. [0003] Modern science has discovered that in a woman's monthly menstrual blood, there is a unique stem cell - endometrial stem cells, whose number and activity are more than 30 times that of autologous bone marrow ...

AI Technical Summary

Problems solved by technology

[0005] 1. The steps are complicated, the process is cumbersome, time-consuming and labor-intensive, and repeated centrifugation operations are required manually, which increases labor intensity and time, and also increases costs.

[0006] 2. Since multiple processes such as collection, separation and culture cannot be intensively completed in a set of equipment, it is easy to be interfered by the external environment, which greatly affects the culture effect of endometrial stem cells

[0007] 3. Unable to realize electronization and automation, lack of quantitative regulation, manual operation is random, which hinders the cultivation and application of large-scale endometrial stem cells

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