Solid tumor treatment system
A tumor, part of the technology, applied in the field of treatment systems for solid tumors, can solve the problems of inaccurate temperature measurement of hyperthermia equipment, poor treatment results, etc., achieve significant ablation effect, and avoid complications.
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[0059] Transplanted tumor model is established:
[0060] Mouse colon cancer cell line CT26 was purchased from the US American Type Culture Collection (ATCC). Culture Conditions: Take the 1640 medium (Hyelone) containing 10% inactivated fetal bovine serum (Hyelone), the cells were placed in a CO2 incubator 37 ° C, 95% air, 5% CO 2 The culture was cultured under conditions, and the concentration of O2 was 21%, and the cultured cells were digested with 0.25% pancreatic enzyme.
[0061] BALB / C male mice are also only 6 to 8 weeks old, with weight 18-20 grams, purchased from Beijing Virong Lihua Experimental Animal Technology Co., Ltd., raised from the First Clinical Medical College Animal Experiment Center of Harbin Medical University.
[0062] After taking the long-term tumor cell CT26, after digestion of the tri-periods, the serum 1640 medium was washed twice, and the cell concentration was resuspended, and the cell concentration was 106 / ml, and tumor cells were extracted with a...
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