Combinational medication
A technology for combining medicaments and compounds, applied in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc.
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[0257] Hereinafter, the present invention will be described in further detail through examples, but the present invention is not limited to these examples.
[0258]
[0259] (S,E)-N-(1-((5-(2-((4-cyanophenyl)amino)-4-(propylamino) pyrimidin-5-yl)-4-pentyn-1-yl)amino)-1-oxopropan-2-yl)-4-(dimethylamino)-N-methyl-2-butenamide, and by The method described in the examples of the above-mentioned Patent Document 2 prepared succinate and used it in the following tests.
[0260]
[0261] As test substances, decitabine (hereinafter, also referred to as Decitabine or DEC), venetoclax (hereinafter, also referred to as Venetoclax or VEN), and a succinate salt of compound A1 were used.
[0262] Decitabine (Tokyo Chemical Industry Co., Ltd. Cat. #A2232), venetoclax (LC Laboratories Cat. #V-3579) and succinate of compound A1 were dissolved in dimethyl methylene Sulfone (DMSO) substance.
[0263] MV-4-11 cells, which are human FLT3-ITD mutation-positive leukemia cell lines, were subcul...
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