Method for constructing ancient DNA library

Abstract

The invention provides a method for constructing an ancient DNA library, and relates to the technical field of ultralow-concentration DNA library construction. The method for constructing the ancient DNA library mainly comprises the following steps: cutting deamination sites of DNA short fragments, dephosphorylating, thermally denaturing, connecting a first joint, fixedly connecting products to magnetic beads, repairing blunt ends, connecting a second joint, purifying and eluting the library, determining library preparation efficiency and library concentration and the like. The blank of ultralow-concentration DNA library construction in an existing commercial DNA library construction kit is filled up, efficient library construction can be effectively carried out on ancient DNA and other highly degraded DNA sequences through DNA single chain library construction, and the method is suitable for trace DNA sequencing and forensic medicine application.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for efficient recovery of gene fragments (DNA) with high purity levels over several months at once through an enzyme process called ligating them together into smaller chains on magnets. These small chained molecules were then used to creature other types of chemical reagents like probes or antibodies.

Problems solved by technology

The technical problem addressed by this patented method for efficiently creating an efficient DNA sample used for studying ancestors or generational changes during time after deteriorating organisms have been discovered through genetic techniques such as molecular biology. This requires developing effective ways to prepare large amounts (millions) of pure DNA that can be analyzed accurately while maintain their original structure and function over long periods of storage without losing important parts like sequences themselves.

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