Multi-fluorescent staining solution as well as preparation method and use method thereof

Abstract

The invention discloses a multiple fluorescent staining solution as well as a preparation method and a use method thereof. Each liter of the multiple fluorescent staining solution is prepared from the following components: 105.5-365 mM of strong base, 0.01-50 g of acid, 5-250 g of binding protein, 0.01-0.2 L of preservative, 0.1-50 L of solubilizer, 0.001-20 L of membrane rupture agent, 0.0194-180 mg of fluorescent staining agent and the balance of sterilized distilled water. According to the method, fungus detection substances and detection substances of other microorganisms except fungi are chelated together, the detection result can be obtained only through one-time smearing, one-time dyeing and one-time color observing, the effect is clearer after dyeing, the dyeing boundary of a target object is more sharpened, and therefore the method is very beneficial to small target object recognition; particularly, bacteria and fungi in microorganisms have small forms, clear boundaries and a sharpened fluorescent staining effect, so that the identification of the target object is facilitated.

Description

technical field [0001] The invention belongs to the technical field of multiple fluorescence detection, in particular to the detection of gynecological vaginal microecology; in particular, it relates to a multiple fluorescence staining liquid and its preparation method and use method. Background technique [0002] The existing gynecological vaginal microecology detection method uses a two-step method to stain the samples, that is, the detection of fungi in each sample must be stained separately. Each sample needs to be coated with two glass slides, one slide is stained with fungi, and is detected by UV fluorescence module; the other slide is stained with microorganisms and other formed components other than fungi, and is detected by blue light fluorescence module; The detection method has long operation time, cumbersome steps, and requires two fluorescence modules, which requires high configuration of imaging equipment. [0003] And at present, the dyeing clarity of similar...

AI Technical Summary

Problems solved by technology

Each sample needs to be coated with two glass slides, one slide is stained with fungi, and is detected by UV fluorescence module; the other slide is stained with microorganisms and other formed components other than fungi, and is detected by blue light fluorescence module; The detection method has long operation time, cumbersome steps, and requires two fluorescence modules, which requires high configuration of imaging equipment

[0003] And at present, the dyeing clarity of similar products to the target is not high, especially the imaging outline of small targets (such as bacteria, fungi, etc.) is not clear, which will cause errors (omissions and mistakes) in the recognition (manual and automatic) process recognition); the current traditional non-staining method (salt water sheet detection) recognition accuracy, due to the limitations of the technology itself, can only reach a maximum of 75%-80%

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