111 results about "Membrane rupturing" patented technology

The present invention relates to the technical field of lithium battery materials. Provided are slurry that in-situ compounds an aromatic polyamide, and a preparation method therefor and the use thereof. The slurry that in-situ compounds an aromatic polyamide achieves the compounding of two materials of nano ceramic particles and an aromatic polyamide, thereby effectively avoiding the problem in the prior art of nano ceramic particles being prone to falling off due to the fact that a binder needs to be added to bond the nano ceramic particles on a separator. A lithium battery separator prepared from the slurry that in-situ compounds an aromatic polyamide further improves the performance, such as peel strength, membrane rupture temperature and electrolyte contact angle, of the lithium battery separator in the prior art. Compared with batteries in the prior art, a battery prepared from the lithium battery separator has an improved capacity retention ratio, and improved normal-temperature capacity, high-temperature capacity (55ºC) and low-temperature capacity (-20ºC).

The invention provides a rapid detection method for cytotoxic T cells in peripheral blood. Specifically, the method provided by the invention is used for rapidly identifying the cytotoxic T cells in the peripheral blood by detecting the expression of cell surface proteins CX3CR1 and GPR56. The method provided by the invention is quick to operate, does not involve cell fixation and membrane rupture, and does not affect cell activity.

The invention provides an agricultural plastic mulching film breaking and collecting all-in-one machine which comprises a machine frame and walking wheels arranged on the lower portion of the machine frame, the machine frame is provided with a fuel generator providing a power source, the front end of the machine frame is provided with a film breaking assembly, the rear end of the machine frame is provided with a film collecting assembly, and the two sides of the front end of the machine frame are each provided with a horizontal displacement assembly. The two horizontal displacement assemblies are connected with lifting assemblies correspondingly, the opposite sides of the bottom ends of the lifting assemblies are connected with fixing plates correspondingly, the two fixing plates are provided with membrane breaking assemblies correspondingly, and each membrane breaking assembly comprises a mounting shaft horizontally connected to the corresponding fixing plate and a membrane breaking blade assembled on the mounting shaft. The rear end of the machine frame is connected with a handrail pushing the machine frame to move forwards, and control parts of the horizontal displacement assembly and the lifting assembly are connected with a controller. The all-in-one machine is reasonable in structural design, convenient to operate and high in film breaking and collecting efficiency, the film breaking distance can be adjusted in real time, and films can be broken and collected accurately.

The present invention relates to the technical field of balloon catheters, and discloses a membrane rupture balloon catheter and a method for using the same. The technical solution thereof mainly comprises a catheter, a membrane rupture hole is formed on the catheter, a membrane rupture mechanism is arranged in the catheter, the membrane rupture mechanism comprises a guide part, the guide part is used to guide a membrane rupture device to the membrane rupture hole, when the membrane rupture device is extended into the catheter, the membrane rupture device moves toward one side of the balloon, the guide part changes the moving direction of the membrane rupture device, the membrane rupture device can be moved to the membrane rupture hole through the guide part, the membrane rupture device can be quickly extended out of the membrane rupture hole, the time for the membrane rupture device to search for the membrane rupture hole in the catheter is reduced, thereby reducing the overall operation time, and the guide groove can determine the direction of the membrane rupture device when it moves out of the membrane rupture hole, so that the operator can further change the direction of the membrane rupture device, and the operation is convenient.

The utility model discloses a special membrane rupturing device for an obstetrical delivery room, which is applied to the technical field of membrane rupturing devices and is characterized in that a fixed cylinder extends into the vagina of a pregnant woman, a protective sleeve is attached to a fetal membrane, then thrust is continuously applied to the fixed cylinder, the protective sleeve and a first spring are compressed at the moment, and a membrane rupturing needle slowly extends out of a through hole and punctures the fetal membrane; when the length of the membrane rupturing device needs to be adjusted, limitation on the telescopic rod is relieved through the fixing mechanism, then the handheld part is pulled to drive the telescopic rod to move, and the telescopic rod is fixed through automatic reset of the fixing mechanism.

The invention provides a high-pressure gas membrane rupture pressure measuring device and a measuring method thereof, and belongs to the technical field of pressure measurement. The measuring device comprises a pressure generation system, a gas compression system, a gas injection system, a pressure measuring system and a diaphragm clamping system. The piston divides the cavity into a hydraulic cavity and a gas compression cavity, and the piston is hydraulically driven to compress the pre-filled gas, so that the diaphragm is broken. The pressure measurement system records pressure-time data in real time, and a sliding window average algorithm is adopted to extract a membrane rupture pressure peak value. According to the technical scheme, inert gas is used, initiating explosive devices are not used, membrane rupture energy is only gas expansion, the protection box can absorb fragments, and explosion-proof transformation is not needed in a laboratory. And the smoothed unique membrane rupture pressure value is output through a sliding window averaging algorithm, and manual reading errors are eliminated. According to the technical scheme, the device is compact in structure, safe to operate, rapid in testing and suitable for accurately measuring the diaphragm rupture pressure of the gas gun diaphragm in a conventional laboratory.

The utility model discloses an electric film breaking device for flue-cured tobacco cultivation, which comprises a top frame, side frames are fixedly connected to the bottom of the top frame close to the left side and the right side, limiting grooves are formed in the sides, close to the center of the top frame, of the side frames, and moving wheels are movably connected to the bottoms of the side frames. The position, close to the center, of the top of the top frame is fixedly connected with a first motor, the bottom of the first motor is in transmission connection with a threaded rod, the bottom of the threaded rod is in bearing connection with a bottom plate, and the position, close to the front face, of the bottom of the bottom plate is movably connected with moving wheels; an air pump is fixedly connected to the position, close to the middle, of the top of the bottom plate, and a collecting box is arranged on the front face of the air pump. According to the mulching film collecting device, the air pump and the air outlet pipe are arranged, so that the time and the labor cost for manually collecting mulching films are reduced, environmental protection is facilitated, and the reliability and the stability of mulching film collection are improved by adopting the mode for collecting the mulching films, so that the cost is saved.

The utility model discloses an artificial membrane rupture device with a uterine orifice measuring function, and relates to the technical field of membrane rupture devices. The device comprises a barrel, a sliding rod is arranged in the barrel in a sliding fit mode, a moving block is arranged at one end of the sliding rod, one side of the moving block is elastically matched with the barrel, and a groove is formed in the other side of the moving block. One end of the membrane breaking piece is provided with a connecting block, and the connecting block is matched with the groove; wherein one end of the barrel body is connected with an installation head in a clamping mode, a cylindrical groove is formed in the installation head, a fixing piece is arranged in the installation head in an elastic fit mode and matched with the connecting block, a storage box is arranged on one side of the barrel body, and a flexible ruler is arranged in the storage box in a sliding fit mode. According to the utility model, the flexible rule can be pulled out to measure the position of the cervix when the cylinder body of the device is inserted for artificial membrane rupture, so that medical personnel can conveniently carry out the subsequent artificial membrane rupture process, and the conditions of discomfort and infection of a puerpera caused by repeated vagina examination are reduced.

The utility model provides an artificial membrane rupture device, which relates to the technical field of assistive devices and comprises a tube body, an adjusting block is arranged on one side of the tube body, a rotating ring is arranged in the middle of the adjusting block, an adjusting component is arranged on the inner ring of the rotating ring, a pressing block is arranged at one end of the tube body, a group of discs are arranged at the bottom of the pressing block, and the disc is arranged on the other end of the tube body. A set of sliding grooves are formed in the inner wall of the pipe body, sliding blocks are arranged in the sliding grooves, the sliding blocks are connected with discs, a jacking assembly is arranged between the discs, and an opening assembly is arranged at the other end of the pipe body. The device solves the problems that the depth of a needle head is inconvenient to control by holding the needle head during manual membrane rupture, a membrane rupture opening is inconvenient to expand after membrane rupture, and amniotic fluid is inconvenient to collect.

The utility model provides an ultrafiltration membrane pressure detection device, which relates to the technical field of ultrafiltration membrane pressure detection, and comprises a shell, the top of the shell is provided with a top cover, the top of the top cover is connected with a drain outlet, the front surface of the shell is connected with a monitoring alarm assembly, and the monitoring alarm assembly comprises a purified water outlet. According to the utility model, the pressure sensor and the flow sensor are respectively arranged at the purified water outlet and the drain outlet, so that a real-time monitoring effect can be achieved, and when the flow or the pressure difference reaches a set numerical value, the PLC controller gives an instruction to carry out systematic backwashing, forward flushing and other maintenance actions on the membrane at the moment; and if the trans-membrane pressure difference almost does not exist, the serious problems such as membrane breakage are possibly caused, and the alarm can automatically give an alarm to remind an operator to check the fault of the membrane in time, so that the problem that the membrane cannot be normally used due to blockage is avoided.

The invention provides an aorta covered stent in-situ windowing puncture membrane rupture set, which relates to the field of medical instruments and comprises a puncture membrane rupture assembly, a membrane expansion assembly and a support outer tube assembly, comprising a core wire, an expansion tube and a supporting guide tube, the portion, close to the far end, of the expansion tube is provided with a plurality of expansion wires which expand outwards in the radial direction in the free state and are in an ellipsoid shape. A plurality of long slots are formed in the part, close to the far end, of the supporting guide pipe; the expansion pipe and the supporting guide pipe can axially slide and / or circumferentially rotate, so that when the expansion pipe and the supporting guide pipe are located at the first relative position, the multiple expansion wires are restrained in the supporting guide pipe by the pipe wall of the supporting guide pipe, and when the expansion pipe and the supporting guide pipe are located at the second relative position, the multiple expansion wires expand out of the supporting guide pipe from the multiple long open seams correspondingly. The aorta covered stent can be applied to in-situ fenestration, after the aorta covered stent fenestration membrane rupture point is accurately positioned, the membrane is quickly ruptured, and the opening after membrane rupture is quickly flared.

The utility model discloses a biodegradable film punching and collecting device, which comprises a punching unit, which comprises a rotating shaft, a punching wheel rotatably arranged on the rotating shaft, an armrest frame arranged between the two ends of the rotating shaft, and punchers arranged on the punching wheel at intervals along the circumferential direction, and each puncher is provided with a film breaking opening; the film collecting unit comprises an air nozzle installed in the film breaking opening, an air pump installed on the punching wheel, an air pipe connected with the air pump and the air nozzle and a film collecting box installed on the lower middle portion of the rear side of the punching wheel, and a film collecting opening is formed in the side, facing the punching wheel, of the film collecting box. Through the arranged film collecting unit, when a tapper rotating to the bottom breaks a film, an air pump sucks air, so that broken residual films are sucked into a film breaking opening, when the tapper rotating to the bottom rotates to a film collecting box, the air pump blows air, so that the residual films in the film breaking opening are blown into the film collecting box to be stored, the situation that the residual films are difficult to collect is avoided, and the aesthetic feeling is improved; and the recycling of resources is realized.

The invention provides a membrane electrode assembly and a preparation method thereof, an electrolytic bath and a water electrolysis hydrogen production system. The membrane electrode assembly comprises a supporting layer and a membrane electrode which are stacked; wherein the supporting layer is made of a porous metal net, the porous metal net is formed by interweaving metal wires, and the wire diameter of the metal wires is 0.5 mm-5. 5mm. The supporting layer is used for supporting the membrane electrode and can provide additional mechanical support for the membrane electrode and reduce deformation of the membrane electrode when the membrane electrode is pressed, and meanwhile, the supporting layer can effectively disperse external pressure, reduce local stress concentration of the membrane electrode and reduce the risk of damage to the membrane electrode, so that the compression resistance of the membrane electrode is improved, and the service life of the membrane electrode is prolonged. And the membrane electrode is not easy to have the problems of membrane rupture, serious deformation and the like under the action of pressure difference.

The invention relates to the technical field of medical tools, in particular to an artificial membrane rupturing structure which comprises a barrel and a suction cup mounted at the top end of the barrel and further comprises a guide seat, a membrane rupturing device, a membrane rupturing device and a membrane rupturing device, the guide seat is mounted in the barrel, and a through hole is formed in the guide seat; the adsorption mechanism is installed in the cylinder body and connected with the guide seat, and the adsorption mechanism enables the suction cup to be adsorbed to the surface of the amniotic membrane; the artificial membrane rupturing structure is different from the prior art, in the using process of medical staff, the amniotic membrane can be clamped and fixed only by attaching the suction cup to the amniotic membrane and then pulling the hollow push rod, then puncturing is automatically conducted, the flow speed after membrane rupturing is stable, the membrane rupturing can be easily completed even though the medical staff with less experience, and operation is easy; the amniotic fluid after rupture of the membrane automatically flows to the outside through the drainage tube from the inside of the cylinder, and the amniotic fluid is not in contact with the uterus of the pregnant woman, so that the probability of infection in the uterus of the pregnant woman is greatly reduced, and the turbidity of the amniotic fluid flowing out can be directly observed.

A separator and a preparation method therefor, and a method for determining the cross-linking degree of a separator. The separator has a TMA membrane rupture temperature of greater than or equal to 170°C, a tensile strength of greater than or equal to 2000 kgf / cm2, and a ductility of greater than or equal to 100%. The preparation method for the separator comprises the following steps: sequentially subjecting a polyolefin base material to dip-coating and a light treatment, so as to obtain a separator, wherein a dip-coating solution for dip-coating comprises a photo-initiator, a synergist and an organic solvent. The separator has a higher membrane rupture temperature and higher high-temperature strength. In the preparation method for the separator, by using a method of using the photo-initiator and the synergist in combination, the initiation efficiency of the photo-initiator is improved, the cross-linking degree of the separator is improved, and the residue of the photo-initiator is reduced, thereby improving the performance of the separator.

The invention relates to the technical field of biological medicines, in particular to a method for evaluating exosomes by counting exosome nanoparticles. According to the nanoparticle counting scheme for evaluating the exosome, exosome samples of different batches in the same preparation method or exosome samples of exosome products of the same batch treated by different methods can be directly evaluated through a membrane rupture particle ratio according to parameters of nanoparticle number change caused by exosome vesicle rupture; besides, the particle number of the exosome sample before and after treatment and before and after membrane rupture is measured, the preservation rate of the treated exosome is calculated according to the measured result, and finally, the treatment effect can be quantitatively evaluated according to the preservation rate of the treated exosome, so that the treated exosome can be more effectively evaluated; therefore, quality detection and quality control are carried out.

The invention relates to a polypeptide and application thereof in preparation of antitumor drugs, and belongs to the technical field of antitumor drugs. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1. The compound can selectively inhibit CRC cells (including CT26, MC38 and HCT116), is non-toxic to other cells (including human intestinal epithelial cells CCD841 and HIEC6), and has relatively high application safety. In addition, the possibility of drug resistance of tumor cells to the polypeptide is reduced through the membrane rupture oncolytic mechanism of the polypeptide. The compound can be used for inhibiting CRC tumor cells so as to realize control and treatment of CRC.

The invention relates to the technical field of obstetrical nursing, in particular to an obstetrical nursing membrane rupture midwifery device with a negative pressure balancing function, which comprises an inserting cylinder, an endoscope is fixedly mounted at the upper part of the rear end in the inserting cylinder, and a membrane rupture assembly is arranged at the rear end in the inserting cylinder. The film breaking assembly comprises a partition plate fixed to the rear end of the interior of the inserting barrel, a plurality of film guiding holes are formed in the partition plate, a cutter groove is formed in the rear end face of the partition plate, and a film breaking blade is movably installed in the cutter groove. According to the amniotic membrane rupturing device, a baffle can block a fetus in an amniotic membrane, so that the soft outer wall of the amniotic membrane enters a membrane guiding hole, the fetus in the amniotic membrane cannot be damaged during rupture of the amniotic membrane, and meanwhile, a membrane rupturing blade is located in an inserting cylinder, so that when rupture of the amniotic membrane is carried out, the fetus can be ruptured conveniently. And the membrane rupture blade does not hurt a fetus or a puerpera.

The utility model discloses a membrane rupture detection device, and belongs to the technical field of corona treatment. The broken film detection device comprises a rack, a detection mechanism, a driving mechanism, a corona mechanism and the like. Wherein the rack comprises a top plate and a bottom plate, at least four supporting columns are arranged between the top plate and the bottom plate, and at least two groups of supporting columns are formed; supporting rollers are arranged on the group of supporting columns; a winding roller is arranged on the other group of supporting columns; a detection mechanism is arranged on the rack; a corona mechanism is arranged between the supporting roller and the wind-up roller, and the corona mechanism is arranged on the side, close to the wind-up roller, of the detection mechanism; the rack is also provided with a driving mechanism, and one end of the driving mechanism is connected with the corona mechanism. Compared with the prior art, the possibility that the corona mechanism is damaged by the film is reduced, so that the production efficiency of the film is improved to a certain extent.

The present invention provides a broad-spectrum antimicrobial peptide AG3 and a preparation method and application thereof, belonging to the field of biotechnology, and the amino acid sequence thereof is shown in SEQ ID No. 1. The antimicrobial peptide AG3 of the present invention has a significant inhibitory effect on 11 Gram-negative and Gram-positive bacteria and fungal species such as Escherichia coli, Pseudomonas aeruginosa, Salmonella pullorum, Staphylococcus aureus, and Candida albicans, and the average antibacterial activity reaches 2 μM; the bactericidal power also shows that it has a high bactericidal ability against bacteria; at the same time, its hemolytic activity is low, and the therapeutic index is as high as 32; in addition, it still has good antibacterial activity in physiological concentration salt ions, indicating that it has a high salt ion tolerance, and related mechanism experiments prove that it realizes antibacterial function through a physical membrane rupture mechanism.

The utility model provides a mixing conversion device and a polyvinyl chloride production system, and relates to the technical field of polyvinyl chloride production. The mixer is provided with a hydrogen chloride inlet, an acetylene inlet, an explosion-proof membrane rupture detection mechanism and a mixed converted gas outlet; the hydrogen chloride conveying pipe is used for connecting a hydrogen chloride synthesis section and a hydrogen chloride inlet; a hydrogen chloride stop valve is arranged on the hydrogen chloride conveying pipe; the acetylene conveying pipe is used for connecting an acetylene synthesis section and the acetylene inlet; an acetylene stop valve is arranged on the acetylene conveying pipe; the acetylene flame arrester is arranged on a path of the acetylene conveying pipe; the acetylene flame arrester is connected with a nitrogen conveying pipe, and a nitrogen stop valve is arranged on the nitrogen conveying pipe; and the acetylene cut-off valve, the hydrogen chloride cut-off valve and the nitrogen cut-off valve are all associated with the explosion-proof membrane rupture detection mechanism. The system comprises the device. According to the utility model, the treatment time for closing corresponding valves is shortened, the escape of hydrogen chloride gas and acetylene gas is avoided, the environmental pollution is reduced, and the safety risk is reduced.

The utility model provides an in-situ windowing membrane rupture device for a covered stent, which relates to the field of medical instruments and comprises an outer tube, a middle-layer positioning tube, an inner-layer positioning tube and a membrane rupture guide wire which are sequentially sleeved from outside to inside and can relatively axially slide two by two, an elastic expansion part is connected to the peripheral surface of the far-end section of the middle-layer positioning tube and has an expansion state of expanding in the radial direction to be spherical and a contraction state of contracting in the radial direction; the outer tube can axially slide relative to the middle-layer positioning tube, so that the elastic expansion part is accommodated or released in the outer tube; the far-end face of the inner-layer positioning pipe is provided with concave-convex positioning sawteeth facing the far-end direction. The aorta covered stent at least relieves the technical problem that in the prior art, when an aorta covered stent is subjected to in-situ windowing and membrane rupture, guide wire positioning is not accurate.

The utility model is applicable to the field of medical instruments, and provides a safe membrane rupture device for obstetrics, which comprises a barrel, a plurality of vent holes are arranged at the upper end of the barrel, a threaded rod is in threaded connection with the upper end of the barrel, an I-shaped ring is fixedly connected with the lower end of the threaded rod, and the I-shaped ring is rotatably connected with a connecting frame. The lower end of the connecting frame is fixedly connected with an abutting plate. A lower bottom plate is slidably connected to the inner wall of the barrel, a membrane rupture needle is fixedly connected to the lower end of the lower bottom plate and used for puncturing amniotic fluid, a middle column is fixedly connected to the upper end of the lower bottom plate, an upper bottom plate is fixedly connected to the upper end of the middle column, and a resistance assembly is arranged on the upper bottom plate in a matched mode. A plurality of symmetrical first vent grooves are formed in the lower bottom plate, a plurality of symmetrical second vent grooves are formed in the middle column, and a plurality of symmetrical third vent grooves are formed in the upper bottom plate. The amniotic fluid puncturing device has the advantages that the amniotic fluid puncturing depth is controllable, and the amniotic fluid is effectively sucked away.

The invention provides a covered stent in-situ windowing and membrane rupture device and a puncture and membrane expansion two-in-one guide wire, and relates to the field of medical instruments, and the device comprises the puncture and membrane expansion two-in-one guide wire and a quick release handle; the puncture and membrane expansion two-in-one guide wire comprises an inner guide wire, a pushing tube and a membrane expansion supporting net. The inner guide wire comprises a core wire and a reducing winding spring; the core wire penetrates through the pushing pipe; the near-end main handle and the far-end main handle slide relatively in the axial direction, and the supporting net rack expands in the radial direction or contracts in the radial direction to the core wire. The guide wire can improve the positioning accuracy of the guide wire at a windowing membrane rupture point of the covered stent and quickly rupture a membrane, can synchronously broach a puncture hole, and meanwhile, can achieve the effect that the guide wire is put in one time and alternately put in a plurality of instruments, thereby achieving the effects of simplifying the operation steps, improving the operation efficiency and reducing the injury to a patient in the operation process.

The invention relates to the technical field of protection of external vesicles, in particular to a preserving fluid for improving the stability of the external vesicles and a preparation method and application of the preserving fluid. The invention provides a preserving fluid for improving stability of external vesicles. The preserving fluid comprises an acidic buffer solution and a stabilizer, the acidic buffer solution is a citric acid buffer solution, and the stabilizer is cyclodextrin or a functionalized derivative thereof; and the pH value of the preservation solution is 6.0-7.0. The preserving fluid provided by the invention can stably store the external vesicles under the condition of low temperature (4 DEG C and-80 DEG C), and can effectively inhibit damage, such as membrane rupture and protein inactivation, of the external vesicles in the low-temperature storage process.

The utility model relates to a capsular membrane polisher after cataract surgery, which comprises a connecting tube, a polishing head is rotatably connected to one end of the connecting tube far away from the connecting tube, a water outlet hole is arranged on the polishing head, the connecting tube is communicated with the water outlet hole, an impeller is arranged at the position of a tube opening where the connecting tube is connected with the polishing head, and the impeller is connected with the polishing head. A connecting rod is fixedly connected to the center of the impeller, the two ends of the connecting rod are rotationally connected with the side wall of the connecting pipe and fixedly connected with the side wall of the polishing head respectively, a pushing and injecting assembly is installed at the end, away from the polishing head, of the connecting pipe and used for injecting liquid into the water outlet hole, and the liquid drives the impeller to rotate when passing through the impeller. The capsular membrane polisher used after the cataract surgery aims to solve the problems that in the prior art, the head of a capsular membrane polisher is fixedly connected with a handle of the capsular membrane polisher, the polisher polishes the capsular membrane excessively due to excessive force in the using process, the safety is not high, the head needs to be rotated manually by workers, and the operation difficulty is large.

The invention provides an aorta covered stent in-situ windowing and membrane rupture system. The aorta covered stent in-situ windowing and membrane rupture system comprises a guide wire, a balloon catheter and a handle capable of being rapidly disassembled and assembled. The guide wire comprises a core wire, a spring wire and a limiting piece. The limiting piece is arranged on the near-end side of the spring wire, and the near end is fixed to the core wire or connected to the middle section of the spring wire. The balloon catheter is sleeved outside the core wire; the far-end end face of the balloon catheter is blocked on the near-end side of the middle expanding section of the limiting piece. The handle capable of being rapidly disassembled and assembled comprises a first handle assembly, a second handle assembly and a locking assembly. The first main handle and the second main handle slide relatively in the axial direction, so that the far-end end face of the balloon catheter can abut against or be away from the middle expanding section of the limiting piece. The guide wire can improve the positioning accuracy of the guide wire at a windowing membrane rupture point of the covered stent and quickly rupture a membrane, can synchronously broach a puncture hole, and meanwhile, can achieve the effect that the guide wire is put in one time and alternately put in a plurality of instruments, thereby achieving the effects of simplifying the operation steps, improving the operation efficiency and reducing the injury to a patient in the operation process.

The invention discloses a predictive screening method of membrane-rupturing antibacterial peptides, and belongs to the technical field of biological information. According to the method, a sequence classifier based on machine learning is developed, high-throughput screening is carried out on proteome, and a polypeptide sequence which can be in contact with a membrane and has a secondary structure mainly being a spiral is input into the sequence classifier to predict whether the polypeptide sequence is an antibacterial peptide sequence or not; and for the sequences screened by the sequence classifier, sequences with positive net charges and relatively hydrophobic are further screened, the novelty of the sequences is verified through sequence comparison, then the membrane contact probability average value and the oligomerization possibility of forming antiparallel dimers through combination are counted, and the antibacterial peptide sequence with the membrane rupture characteristic is screened out. The accuracy of predicting the antibacterial peptide by the sequence classifier based on machine learning developed by the invention is superior to that of an existing method, a series of membrane-ruptured antibacterial peptides are successfully obtained through the screening method, and the membrane-ruptured antibacterial peptides have good antibacterial activity and low toxicity and are ideal antibacterial drug development objects.

This invention relates to the field of obstetric nursing technology, and in particular to an obstetric nursing device for ruptured membranes with a negative pressure balancing function. The device includes an insertion tube, an endoscope fixedly mounted on the upper rear end of the insertion tube, and a membrane rupture assembly located at the rear end of the insertion tube. The membrane rupture assembly includes a baffle plate fixed to the rear end of the insertion tube, with multiple guide membrane holes inside the baffle plate. A blade groove is formed on the rear end face of the baffle plate, and a membrane rupture blade is movably mounted inside the blade groove. The baffle plate of this invention effectively blocks the fetus inside the amniotic sac, allowing the soft outer wall of the amniotic sac to enter the guide membrane holes, thus preventing damage to the fetus during membrane rupture. Simultaneously, the membrane rupture blade is located inside the insertion tube, ensuring that neither the fetus nor the mother is harmed during membrane rupture.

An effective and safe composition comprises an anionic gold-polydopamine core-shell nanoworm as an alternative gene carrier for bypassing the bottleneck of endosomal entrapment. The nanoworm can be used in methods of delivering therapeutic oligonucleotides to a subject. A polydopamine shell supports the surface adsorption of nucleic acids. The anionic nucleic acid-encased nanoworm can then enter cells without transfection agents and activate the ClC3 H + / Cl - exchanger in late endosomes to mediate vesicular accumulation of H + and Cl -, which causes membrane rupture, and finally escape to cytosol without cell-penetrating peptides or mechanical stimuli. The nanoworm can be further used for programming cellular responses, i. e., primary macrophage polarization and stem cell differentiation, for the treatment of diseases, such as kidney fibrosis and acute liver injury.