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Anisodine separated and purified from alkaloid extract and preparation method thereof

A technology for separation and purification of alkaloids, applied in the direction of organic chemistry, etc., can solve the problems of affecting the efficacy of anisodine and low purity of anisodine

Inactive Publication Date: 2021-10-26
锦州拾正生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purity of anisodine obtained by traditional extraction methods is not high, which will affect the efficacy of anisodine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0017] In one aspect, the present invention provides a preparation method for separating and purifying anisodine from alkaloid extract, comprising the following steps: obtaining alkaloid extract from Huashan ginseng by extraction method, dissolving alkaloid extract in water and using MCI Column chromatography, eluting with a mixture of methanol / acetonitrile / water to obtain eluent 1, eluent 1 undergoes 200-300 mesh silica gel column chromatography, and elutes with chloroform / methanol solution to obtain eluent 2, The eluent 2 was eluted by acetone / petroleum ether solution chromatography to obtain anisodine.

[0018] Impregnating Huashan ginseng with the extractant to extract the active ingredients in the extractant, and then filtering and concentrating to obtain the alkaloid extract. The soluble active ingredients in the alkaloid extract are dissolved in water, and then 80-100 mesh MCI gel is added into the mixed liquid for stirring, and then poured into a glass column for chrom...

Embodiment 1

[0031] The embodiment of the present invention proposes a preparation method for separating and purifying anisodine from alkaloid extract, comprising the following steps:

[0032] Pretreatment: Grind and sieve Huashan ginseng with a mesh number of 80 mesh.

[0033] Extraction of alkaloids: immerse the above-mentioned pretreated Huashan ginseng at a temperature of 40°C with ethanol with a volume fraction of 95% vol, then filter to obtain the filtrate and filter residue, then add the above-mentioned ethanol to the filter residue for impregnation, and filter again, so The Huashan ginseng is extracted by circular operation. In addition, ultrasonic treatment is performed during the impregnation.

[0034] Preparation of alkaloid extract: use the above method to extract Huashan ginseng 4 times, then combine the filtrates, concentrate the mixed filtrate under reduced pressure to obtain extracts, adjust the pH of the extracts to 3, let stand for 6 hours, and then adjust After the pH ...

Embodiment 2

[0038] The embodiment of the present invention proposes a preparation method for separating and purifying anisodine from alkaloid extract, comprising the following steps:

[0039] Pretreatment: Grind and sieve Huashan ginseng with a mesh number of 100 mesh.

[0040] Extraction of alkaloids: impregnate the above-mentioned pretreated Huashan ginseng at a temperature of 35°C with ethanol with a volume fraction of 90% vol, then filter to obtain the filtrate and filter residue, then add the above-mentioned ethanol to the filter residue for impregnation, and filter again, so The Huashan ginseng is extracted by circular operation. In addition, ultrasonic treatment is performed during the impregnation.

[0041] Preparation of alkaloid extract: use the above method to leach Huashan ginseng 3 times, then combine the filtrates, concentrate the mixed filtrate under reduced pressure to obtain extracts, adjust the pH of the extracts to 1, let stand for 5 hours, and then adjust The origina...

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Abstract

The invention provides anisodine separated and purified from alkaloid extract and a preparation method thereof, and relates to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: acquiring an alkaloid extract from physochlaina macrophylla by using an extraction method, dissolving the alkaloid extract in water, carrying out MCI column chromatography, performing eluting by using a methanol / acetonitrile / water mixed solution to obtain an eluate I, carrying out silica gel column chromatography on the eluate I by using a 200-to-300-mesh silica gel column, then performing eluting by using a chloroform / methanol solution to obtain an eluate II, and carrying out chromatographic elution on the eluate II by using an acetone / petroleum ether solution to obtain anisodine. The preparation method is simple to operate and suitable for large-scale production, the used eluates, chromatographic solution and the like can be recycled, and production cost can be reduced while resources are saved. The invention further relates to the anisodine which is prepared by using the preparation method, and the anisodine is relatively good in quality and physiological activity, relatively high in purity, good in medicinal effect and relatively high in medical value.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to anisodine separated and purified from alkaloid extracts and a preparation method thereof. Background technique [0002] Anisodine is one of the components of tropine alkaloids, also known as 1-hydroxyscopolamine, is an alkaloid extracted from Huashan ginseng, referred to as AT-3, its chemical structure is similar to that of anisodine and scopolamine resemblance. Anisodine has a strong central anticholinergic effect, which can inhibit the cerebral cortex and block the cholinergic nerves to the periphery, relieve vasospasm, improve microcirculation and local nutrient metabolism in the lesion area, Make the mucosa of the lesion area achieve normal keratinization, promote the recovery of inflammation, and have the functions of eliminating oral plaques. More importantly, the toxicity of anisodine is smaller than that of other anticholinergic drugs such as scopola...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D451/10
CPCC07D451/10
Inventor 刘东滨于喜洋刘千驰
Owner 锦州拾正生物科技有限公司
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