Construction method of transgenic zebrafish (mbp:egfp) spinal cord removal disease model
A disease model and construction method technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as difficult to control the curative effect, aggravation of neurological symptoms, and incomplete understanding of the etiology, and achieve a simple construction method , the intuitive effect of the research results
Pending Publication Date: 2021-11-02
ZHEJIANG POLICE COLLEGE
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Problems solved by technology
The early treatment is mostly with hormone and nutritional therapy, but the curative effect is difficult to control. Because the disease leads to demyelination and severe neurological damage, secondary axonal damage leads to recurrence, which further aggravates neurological symptoms.
[0003] The factors that lead to demyelinating lesions are complex, and both genetic and environmental factors may contribute to the occurrence of the disease, but the etiology is not fully understood, which also poses difficulties for the improvement of its treatment methods
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Embodiment 1
[0018] Drug induced by addition of scopoletin in zebrafish generated from myelin mbp transgenic zebrafish.
[0019] (1) mbp transgenic zebrafish, zebrafish purchased from the National Resource Center.
[0020] (2) using a confocal microscope imaging, we found 2dpf (days post fertilization, fertilized acquired) Phases mbp transgenic zebrafish, in 3dpf period found scopletin treated group significantly myelin sheaths relative to control and solvent control group developmental damage ( figure 1 .
Embodiment 2
[0022] In mbp transgenic zebrafish 5dpf drug treatment, it was found with respect to the solvent control group and the control group showed significant decreases in activity 6dpf scopoletin treatment groups ( figure 2 .
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The invention discloses a construction method of a transgenic fish (mbp:egfp) demyelinating disease model established by using a drug scopoletin, and the method comprises the following steps: placing the transgenic fish (mbp:egfp) in a 18.5 [mu] g / ml scopoletin solution, and completing the construction of a zebra fish model after 3-6 days. By combining various factors such as genes, environment, aging and the like, the zebrafish demyelination disease model can be quickly constructed, and a basis is provided for a mechanism and a treatment method of the demyelination disease.
Description
Technical field [0001] The present invention is in the field of biomedicine, in particular to a zebrafish gene transfer: spinal cord removal method of constructing a disease model (mbp egfp). Background technique [0002] Before the onset of demyelinating disease is an acute or subacute disease damages the nerve center, the peak incidence is two to three weeks, if the delay in treatment of damaged nerve degeneration secondary to ischemia and multiple sclerosis occurs, the incidence of serious violations of the spinal cord can horn cells and brainstem nucleus and the motor cortex pyramidal cells endanger the lives, mostly due to genetic abnormalities or immune virus infection. Early in the treatment of multiple hormonal and nutritional therapy, but the effect is difficult to control the solid, because the disease leads to demyelination induced nerve damage secondary to axonal damage so serious that the recurrence of neurological symptoms further. [0003] Factors leading to demyel...
Claims
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IPC IPC(8): C12N15/12C12N15/85A01K67/027
CPCC07K14/43595C12N15/8509A01K67/0278A01K2207/15A01K2217/072A01K2227/40A01K2267/0356
Inventor 王斌杰陈天逸付立斌叶昕宇徐帆吴元钊许中石姚伟宣王继业
Owner ZHEJIANG POLICE COLLEGE
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