Method and kit for screening gastric cancer related gene mutation

Abstract

The invention discloses a method and kit for screening gastric cancer related gene mutation, and belongs to the technical field of gene mutation detection. The method and the kit use two sets of PCR primers: the first set of PCR primers comprises 61 pairs of PCR primers, the first set of PCR primers comprises 61 pairs of PCR primers (SEQ ID NO.1-SEQ ID NO.61), and the 61 pairs of PCR primers cover 63 sites of gastric cancer related gene mutation; and the second set of primers are universal primers I GT-I5I ndex (SEQI D NO.17) and I GT-I7I ndex (SEQ ID NO.18). The kit comprises a PCR (Polymerase Chain Reaction) primer combination, an I ndex linker sequence, an I GT-EM808 polymerase mixture, an amplification buffer enhancer NB, a YF magnetic bead rinsing buffer solution B, a reagent M and the like. Compared with the prior art, the detection method for detecting the gastric cancer related gene mutation disclosed by the invention has the advantages that the required quantity of a sample to be detected is low; an extremely low DNA sample in plasma and serum can be analyzed; through the design of the carpet type primer, mutation detection of a full coding region can be realized, known mutation can be analyzed, and new mutation can be found; the cost is low; the sensitivity is high; and the detection time is short.

Description

technical field [0001] The invention belongs to the technical field of gene mutation detection, and in particular relates to a method and a kit for screening gastric cancer-related gene mutations. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world. The annual incidence of gastric cancer worldwide is 17.6 / 100,000, ranking second in the mortality rate of malignant tumors. Studies have shown that the incidence of gastric cancer can vary by 7-10 times between high-incidence areas and low-incidence areas, and there are significant differences between regions. Gastric cancer mortality rates in Japan rank first in the world, followed by Chile and Costa Rica, and this trend is true for both men and women. Gastric cancer mortality rates are lower in New Zealand, Denmark, and Australia, the lowest in the United States, and about seven times higher in Japan. The incidence of gastric cancer in Japan has decreased in recent years, but i...

AI Technical Summary

Problems solved by technology

Therefore, there will be a large deviation in the prediction of the occurrence and development of gastric cancer, and there will be great limitations in its use.

Images