A kind of liquid-liquid-liquid three-phase solvent system extracts and separates the method of natural product and its application

Abstract

The invention discloses a method for extracting and separating natural products with a liquid-liquid-liquid three-phase solvent system, which is characterized in that it includes selecting at least four solvents according to the target compound to be extracted, and mixing the selected solvents in different proportions to form a three-phase solvent The steps of the system; after the crude extract of the natural product to be extracted is dissolved in the three-phase solvent system and the phase separation is completed, the step of calculating the distribution ratio of the target compound in each phase; the same The steps of the ratio of the oil in the three phases; according to the requirement that the oil is maximized in one phase and the target compound is enriched in the other phase, the step of screening the most suitable three-phase solvent system; using a three-phase solvent The system extracts microbial fermentation products 1-2 times, and completes oil removal and target compound enrichment at the same time. The advantages are good separation selectivity and high recovery efficiency of target compounds.

Description

technical field [0001] The invention relates to a method for separating and purifying microbial fermentation products, in particular to a method for extracting and separating natural products with a liquid-liquid-liquid three-phase solvent system and its application. Background technique [0002] In the process of microbial fermentation to produce secondary metabolites such as erythromycin, cephalosporins, etc., soybean oil, cottonseed oil, etc. are often added to the substrate as a slowly utilized carbon source. After the fermentation cycle is over, this part of the oil is often not fully utilized and remains in the microbial fermentation product, which brings great difficulties to the subsequent separation and purification of the target compound. At present, the oil removal methods include hot alkali method, organic solvent method, surfactant method, flocculant sedimentation method and so on. The above are mostly used for oily wastewater, degreasing of oil-coated mechanic...

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Problems solved by technology

At the same time, this method also has disadvantages such as poor separation selectivity, which restricts its application in the separation and purification of natural products.

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