Fermentation preparation method of salmonella pullorum bacteriophage lyase

A technology of Salmonella phage and Salmonella pullorum, applied in the field of biology, can solve the problems of poor antibacterial effect, poor cracking effect of Salmonella pullorum phage lyase, poor stability, etc., and achieve acid-base resistance Wide range, no loss of enzyme activity, good thermal stability
CN113604458APending Publication Date: 2021-11-05JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2021-11-05

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Abstract

The invention belongs to the technical field of biology, and discloses a fermentation preparation method of salmonella pullorum bacteriophage lyase, which comprises: culturing salmonella pullorum and salmonella bacteriophage YSP2, and extracting a salmonella bacteriophage YSP2 genome; carrying out polymerase chain reaction (PCR) amplification on a lyase target gene LySP2 by utilizing a primer LySP2-ZF / LySP2-ZR, by taking a whole genome of the bacteriophage YSP2 as a template; constructing and identifying an expression vector pPICZ-LySP2, and screening and identifying a recombinant pichia pastoris strain X33-pPICZ-LySP2; simultaneously carrying out induced fermentation and condition optimization of the recombinant pichia pastoris X33-pPICZ-LySP2. According to the present invention, the pichia pastoris expression strain X33-pPICZ-LySP2 of a recombinant lyase LySP2 gene is constructed.
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Description

technical field

[0001] The invention belongs to the technical field of biology, and in particular relates to a fermentation preparation method of Salmonella pullorum phage lyase. Background technique

[0002] At present, pullorum is one of the 14 kinds of animal diseases that the Ministry of Agriculture of my country requires to focus on monitoring. The harm of its pathogenic bacteria to poultry seriously restricts the development of poultry industry. Purification of pullorum in chicken farms is the best way to eradicate the disease. Antibiotics are traditional drugs for the prevention and treatment of pullorum, but due to the long-term widespread use and even abuse of antibiotics, drug resistance is becoming more and more serious, making the prevention and removal of pullorum more difficult. As new antimicrobial agents, phages and their lytic enzymes have been widely studied and applied in improving food and medical safety. Therefore, it is of great significance to screen ...

Claims

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