Preparation method of immune vaccine for resisting porcine epidemic diarrhea virus variant shxx1902

Abstract

The invention relates to a preparation method of an immune vaccine for resisting a porcine epidemic diarrhea virus variant shxx1902. The preparation method comprises the following steps of culturing vero cells; a virus culture step: adding the cell culture fluid of the porcine epidemic diarrhea virus variant shxx1902 into the vero cell culture fluid, carrying out dilution passage, and culturing for 72 hours for later use; an immunogen preparation step: adding 0.2% formaldehyde into the virus liquid, and inactivating the virus at 37 DEG C; then adding into a 96-well plate paved with vero cells, and culturing in a CO2 incubator; collecting a cell supernatant, fixing the cells by using cold acetone, adding the collected supernatant into a 96-well plate paved with vero cells, and continuously culturing; and removing the supernatant, fixing the cells with cold acetone, adding an alumina gel adjuvant after inactivation verification is qualified, and mixing for use. According to the virus titer of the inactivated immunogen obtained by the method disclosed by the invention, an antibody similar to a commercial vaccine level can be generated after mice are immunized.

Description

technical field [0001] The invention belongs to the field of biology and relates to a method for cultivating bacterial strains, in particular to a method for preparing an immune vaccine against porcine epidemic diarrhea virus mutant strain shxx1902. Background technique [0002] Porcine epidemic diarrhea (porcine epidemic diarrhea, PED) is an acute, highly contagious intestinal infectious disease caused by porcine epidemic diarrhea virus (PEDV). The disease was first reported in the United Kingdom in 1971, and then broke out in Belgium, the United Kingdom, Japan and other countries. In 1984, Chinese scholars reported that PEDV was isolated; then PED occurred in various parts of my country, and in 1995, the classic vaccine strain CV777 inactivated vaccine or attenuated vaccine was used for prevention and control, and good prevention and control effect was achieved. Since 2010, most of the pig farms in the country, including pig farms immunized with classic vaccine strains (s...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a preparation method of an immune vaccine against porcine epidemic diarrhea virus variant strain shxx1902, and the immune vaccine against porcine epidemic diarrhea virus variant strain shxx1902 The preparation method of the vaccine should solve the technical problem that the immune effect of the immunization vaccine against porcine epidemic diarrhea virus mutant strain shxx1902 in the prior art is not good

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