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Cell sap glucose content detection method

A detection method, glucose technology, applied in the field of detection of glucose content in cell fluid, can solve the problems of high detection cost, limited instrument throughput, cell fluid sample detection, etc., to improve the efficiency of feeding, simplify the detection steps, and improve the mixing efficiency Effect

Inactive Publication Date: 2021-11-09
杭州皓阳生物技术有限公司
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AI Technical Summary

Problems solved by technology

Although the instrument detection is highly automated and convenient, it is difficult to complete the detection of a large number of cell fluid samples at the same time due to the limited throughput of the instrument.
In addition, the use of biochemical analyzers to detect Glucose is limited by the instrument, and supporting reagents, calibrators, and quality controls are required, and the cost per unit cell fluid sample is relatively high
Although the cost of the ultraviolet method is relatively low, it requires a relatively large amount of cell fluid samples, which is not conducive to the use of small volume cell culture.
[0004] The disadvantages of these existing technologies are: the detection cost is high, the amount of cell fluid samples is large, and the detection of a large number of cell fluid samples cannot be completed at the same time, and the use is limited by instruments, consumables, resources, etc.; The cell fluid sample can quickly, accurately and massively complete the reaction solution and detection method of glucose content detection, and the present invention solves such problems

Method used

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  • Cell sap glucose content detection method

Examples

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Embodiment Construction

[0044] Such as Figure 1-16 Shown, a kind of cell liquid glucose content detection method comprises the following steps:

[0045] a. Add 200ul of buffer and 20ul of cell fluid sample and standard to the dilution plate;

[0046] b. First inject the developer, GOD, HRP and buffer into the mixer respectively, and then manually adjust the dosage of the developer, GOD, HRP and buffer in a ratio of 6:3:1:1; Put the used test tube on the mixer; start the mixer, the mixer will automatically drop the color developer, GOD, HRP and buffer into the mixing test tube in sequence; at the same time, the test tube will be shaken and mixed to prepare the glucose detection reaction solution;

[0047] c. Add 100ul of glucose detection reaction solution to each well of the detection plate, and then add 20ul of cell fluid sample and standard to the well containing the glucose detection reaction solution;

[0048] Place the reaction solution containing the cell fluid sample and the standard produc...

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Abstract

The invention discloses a cell sap glucose content detection method. The method comprises the following steps: a, adding a buffer agent, a cell sap sample and a standard substance into a dilution plate; b, preparing a glucose detection reaction solution; c, adding a glucose detection reaction solution into the detection plate; d, detecting the glucose concentration of the cell sap sample by a microplate reader, and calculating the glucose content of the cell sap sample, wherein the mixing machine comprises a main body, a motor, a rotating frame, a bracket, a feeding device, a rotating roller, a rotating disc and a mixing device; according to the method, glucose content detection can be completed by using a small amount of cell sap sample in the cell culture process, pretreatment work such as centrifugation is not needed, and detection steps are simplified; the determination can be completed by the microplate reader, and the operation is simple; meanwhile, the mixing device is adopted when the glucose detection reaction solution is prepared so that the mixing efficiency can be improved; and quantitative feeding can be realized by adopting the feeding device, so that the preparation effect is improved.

Description

technical field [0001] The invention belongs to the technical field of detection of glucose content in cell fluid, and in particular relates to a detection method for glucose content in cell fluid. Background technique [0002] Glucose, an organic compound with the molecular formula C6H12O6, is the most widely distributed and most important monosaccharide in nature. Glucose plays an important role in the field of biology. It is the energy source and metabolic intermediate product of living cells, and it is also the main energy supply material of cells. In cell culture, especially in batch-fed experiments, the monitoring and timely supplementation of glucose is the basis for maintaining cell survival. [0003] At present, the glucose content in laboratory cells is mainly detected by ultraviolet spectrophotometer or automatic biochemical analyzer. The detection principle is mainly based on glucose oxidase method, hexokinase method, glucose oxygen electrode method, etc. Altho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/3103G01N21/78
Inventor 李浩强唐慧娴王一茹董宇童
Owner 杭州皓阳生物技术有限公司
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