The invention discloses a cell sap glucose content detection method. The method comprises the following steps: a, adding a buffer agent, a cell sap sample and a standard substance into a dilution plate; b, preparing a glucose detection reaction solution; c, adding a glucose detection reaction solution into the detection plate; d, detecting the glucose concentration of the cell sap sample by a microplate reader, and calculating the glucose content of the cell sap sample, wherein the mixing machine comprises a main body, a motor, a rotating frame, a bracket, a feeding device, a rotating roller, a rotating disc and a mixing device; according to the method, glucose content detection can be completed by using a small amount of cell sap sample in the cell culture process, pretreatment work such as centrifugation is not needed, and detection steps are simplified; the determination can be completed by the microplate reader, and the operation is simple; meanwhile, the mixing device is adopted when the glucose detection reaction solution is prepared so that the mixing efficiency can be improved; and quantitative feeding can be realized by adopting the feeding device, so that the preparation effect is improved.