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Kit and method for identifying myospalax psilurus

A technology of kits and reagents, applied in the field of identification, can solve problems such as the difficulty of accurate identification of species, and achieve the effect of low sequencing length requirements and accurate and reliable results

Pending Publication Date: 2021-11-12
QINGHAI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the similar living environment and similar morphology of different zokors, it is very difficult to accurately identify species through morphology without professional training.

Method used

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  • Kit and method for identifying myospalax psilurus
  • Kit and method for identifying myospalax psilurus
  • Kit and method for identifying myospalax psilurus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, the present invention distinguishes the kit of northeastern zokor

[0052] The components of the kit of the present invention include:

[0053] (1) PCR amplification reagents: primer pairs comprising SEQ NO: 1-2; (2) Reagents for sequencing.

Embodiment 2

[0054] Embodiment 2, the present invention distinguishes the kit of northeastern zokor

[0055] The components of the kit of the present invention include:

[0056] (1) PCR amplification reagents: primer pairs comprising SEQ NO: 3-4; (2) Reagents for sequencing.

Embodiment 3

[0057] Embodiment 3, the present invention distinguishes the kit of northeastern zokor

[0058] The components of the kit of the present invention include:

[0059] (1) PCR amplification reagent: primer pair comprising SEQ NO: 1-2 and SEQ NO: 3-4; (2) Reagent for sequencing.

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Abstract

The invention provides a kit for identifying myospalax psilurus. The kit comprises a reagent for detecting any one or more of the following three SNP sites of species to be detected: the 21st site of a conserved motif sequence shown as SEQ ID NO: 5 in a 12S rRNA gene, the 4th site of the conserved motif sequence shown as SEQ ID NO: 6, and the 25th site of the conserved motif sequence as shown in SEQ ID NO: 7 in the 16S rRNA gene. The invention provides the kit for simply, conveniently and accurately identifying the species of the myospalax psilurus and the method for identifying the myospalax psilurus, the problem of identifying the species of the myospalax psilurus through forms is solved, and the kit and the method have excellent application prospects in species identification of the myospalax psilurus.

Description

technical field [0001] The invention belongs to the technical field of identification, and in particular relates to a kit and a method for identifying northeast zokors. Background technique [0002] Zokor is a collective name for animals in the subfamily Myospalacinae, and it is a rodent adapted to living underground. The body is thick and cylindrical, the head is wide and flat, the head has tentacles, the nose is flat and blunt, with exposed nose pads, the tail and limbs are short, the eyes are very small, the eyesight is poor, almost covered by hair, the auricles are degenerated, hidden in the Under the hair. Zokors are mainly distributed in my country, and are also found in Mongolia and Siberia. They inhabit forest edges, grasslands and farmland. They live in burrows during the day and occasionally go to the ground at night. They feed on roots, stems and seeds of plants and store them in caves. Lots of food. Zokors dig burrows very fast, with a complex burrow system and...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 汪海静覃雯刘道鑫宋鹏飞于瑶迟翔文张婧捷江峰李文君
Owner QINGHAI UNIVERSITY
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