Integrated nucleic acid analysis chip

A technology for analyzing chips and integrating nucleic acids, used in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc. Efficiency and reaction accuracy, contamination avoidance, flexible configuration effects

Pending Publication Date: 2021-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these devices still have some defects such as large size, expensive price, and complicated design, which cannot fully meet the needs of the grassroots.

Method used

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  • Integrated nucleic acid analysis chip
  • Integrated nucleic acid analysis chip
  • Integrated nucleic acid analysis chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Specifically, such as Figure 1 to Figure 5 As shown, the nucleic acid analysis chip includes: a chip 100 , a sliding rail 200 , a piston pump 300 , a catheter 400 , a storage tube 500 , a sealing film 600 , a sealing gasket 700 , and a conduction valve 800 .

[0079] Wherein, the storage tubes 500 are used to store relevant reagents; the piston pump 300 is used to transfer and stir the reagents in the storage tubes 500 ; the slide rail 200 is used to move the conduction valve 800 .

[0080] The chip 100 is provided with a waste liquid chamber 102 and a reaction chamber 104, which are respectively used for pre-storing waste liquid and reaction reagents. The waste liquid chamber 102 is set as a chamber with a relatively large capacity, which is only for discharge, not discharge. A ventilation hole 103 is opened on the chip 100 . The storage tube 500 is fixed on the chip 100 by the storage tube interface 105 . The top of the storage tube 500 is provided with a cap 501 t...

Embodiment 2

[0095] The specific operation process of using this nucleic acid analysis chip to realize integrated nucleic acid analysis is introduced below:

[0096] Such as image 3 The nucleic acid analysis chip shown comprises seven storage tubes 500 in total, numbered 500a to 500g from left to right. Such as Figure 4 As shown, the liquid flow pipes 101 communicating with the storage tube interface 105, the reaction chamber 104, the waste liquid chamber 102, and the vent hole 103 are numbered 101a to 101i from left to right.

[0097] Such as Figure 1 to Figure 5 As shown, before use, place the sample, lysis binding reagent and micro-nano magnetic spheres in the storage tube 500a, a magnet can be placed next to the storage tube 500a for absorbing the micro-nano magnetic spheres; place the magnetic sphere cleaning reagent in the storage tube 500b, Place the chip washing reagent in the storage tube 500c, place the elution reagent in the storage tube 500d, place the dilution reagent in...

Embodiment 3

[0124] Take the double detection of CaMV35S sequence and Lectin sequence in transgenic soybean leaves as an example.

[0125] Such as Figure 1 to Figure 5 As shown, the material used for most of the chip is polymethyl methacrylate, the piston pump is a 1 ml disposable medical syringe, and the material used for the storage tube is polypropylene.

[0126] Nucleic acid amplification adopts the isothermal amplification method, and the nucleic acid amplification reagents are: 16 units of Bst DNA polymerase, 5 microliters of 10xThermopol reaction buffer, 0.8 moles of betaine per liter, 0.35 millimoles of dNTP, and 2 millimoles of magnesium sulfate Per liter, primer mixture (including 4 inner primers, 2 inner primers each 1.6 micromoles per liter, 2 inner primers each 0.8 micromoles per liter; 4 outer primers, 2 outer primers 0.2 micromoles each liter, 0.1 micromol per liter for each of the 2 outer primers; 0.1 micromol per liter for each of the 2 outer primers; 0.4 micromol per li...

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Abstract

The invention discloses an integrated nucleic acid analysis chip. The chip is provided with a vent hole, a reaction cavity, a waste liquid cavity and other cavities, and is also provided with a storage tube connection interface, a slide rail interface, a liquid flow pipeline, an exhaust pipeline and the like, and is provided with a plurality of pipeline ports, wherein the cavities are communicated with one pipeline port through respective pipelines; a storage tube is arranged on the chip through the interface to serve as a cavity; a slide rail and the chip are tightly fixed through the slide rail interface, and a conduction valve slides in the slide rail and is used for selective communication between the cavity and a piston pump; and the piston pump is hermetically connected with the conduction valve through a conduit. The integrated nucleic acid analysis chip can complete the complete steps of nucleic acid extraction, amplification and detection in the chip, can effectively avoid possible pollution, and can better remove reagents having an inhibiting effect on subsequent amplification, thereby avoiding the influence of the reagents on amplification and improving the efficiency and accuracy.

Description

technical field [0001] The invention relates to a chip for nucleic acid experiments in the field of nucleic acid analysis and detection, in particular to an integrated nucleic acid analysis chip. Background technique [0002] Nucleic acid is the carrier of the genetic information of organisms. By detecting nucleic acids, organisms can be accurately identified. At present, nucleic acid analysis and detection have been widely used in food safety testing, environmental monitoring, medical diagnosis and other fields, and play an important role. The nucleic acid detection method based on nucleic acid amplification technology is the most commonly used nucleic acid analysis and detection method, but its detection process often includes complex nucleic acid extraction operations, which requires repeated pipetting, and is prone to cross-contamination during the liquid pipetting process. In order to simplify the nucleic acid extraction steps, some researchers directly perform nuclei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/6844C12Q1/6806C12Q1/6837
CPCC12Q1/6844C12Q1/6806C12Q1/6837
Inventor 吴坚伍辉钱斯雯洁
Owner ZHEJIANG UNIV
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