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Skin repair promoting peptide as well as preparation method and application thereof

A technology for skin repair and skin damage, applied in the preparation method of peptides, preparations for skin care, skin diseases, etc., can solve the problems of unstable biological activity, incompatible dispersibility of polypeptides, unstable biochemical properties, etc. Skin repair, accelerated scar repair, low production cost effect

Active Publication Date: 2021-11-26
四川丽妍工坊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, these peptides not only have the problem of being incompatible with the dispersibility in water and lipid substances, but also have the problem of biochemical instability after being compatible with commonly used cosmetic excipients. Hidden danger
In addition, the biological activity in the preparation of cosmetics is unstable

Method used

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  • Skin repair promoting peptide as well as preparation method and application thereof
  • Skin repair promoting peptide as well as preparation method and application thereof
  • Skin repair promoting peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] This example provides the preparation method of the skin repair promoting peptide (the skin repair promoting peptide in the following examples and experimental examples is named after TYL).

[0065] It includes the following steps in order:

[0066] (1) Synthesize the crude polypeptide by solid-phase synthesis according to the following primary structure, connect the 2nd and 9th cysteines, and form a pair of disulfide bonds;

[0067] TYL: Lys-Cys-Trp-Arg-Trp-Lys-Arg-Trp-Cys-Lys,

[0068] (KCWRWKRWCK, SEQ ID NO. 1).

[0069] (2) Purify: the crude polypeptide is desalted and purified by HPLC reverse-phase column chromatography, and its purity is identified until the purity of the polypeptide is not less than 95%;

[0070] HPLC purification and identification method: Dissolve 0.1mg of the sample to be tested in 1mL ultrapure water containing 0.1% trifluoroacetic acid. If there are undissolved impurities, filter them with a 0.45μm filter membrane. Mobile phase A is 0.1% t...

experiment example 1

[0075] In this experimental example, the stability of the skin repairing peptide TYL prepared in Example 1 was determined.

[0076] In order to test the stability of TYL in plasma, the peptide was first diluted in mouse plasma (using EDTA-K2 as an anticoagulant for 8-week-old male C57BL / 6 mice) to a final concentration of 10ug / ml at 37°C After incubation for 0, 2, 4, 6, 8 and 10 h, aliquots of 10 μl samples were mixed with 4% H 3 PO 4 For protein precipitation, the mixture was vortexed for 2 min and then centrifuged at 13000 rpm for 15 min at 4 °C. Inject 3 microliters of the supernatant for liquid chromatography-tandem mass spectrometry detection (LC / MS / MS, ExactivePlus, Thermo Scientific) for checking.

[0077] The results showed that TYL had better stability in serum. Incubated for 10 hours without a large amount of degradation ( figure 1 ).

experiment example 2

[0079] In this experimental example, the dispersibility of the skin repairing peptide TYL prepared in Example 1 in lipid substances was measured.

[0080] Take the determination of the homogeneity and dispersion of peptides in lanolin as an example. Take 1000g of lanolin and 1000mg of polypeptide, and mix them evenly with a SHW / R mobile high-shear emulsifier at room temperature at a stirring speed of 120 rpm for 30 minutes. After mixing, aliquot into 5ml tubes. The theoretical content under this condition is 1 mg / g. Take and pack into 20 tubes, accurately weigh an appropriate amount of 1g of the mixture (equivalent to about 0.1mg 10ml) into a measuring bottle, add 20% ethanol solution to dissolve it (ultrasound to dissolve if necessary) and dilute to the mark, accurately measure 2ml with Folin Protein quantification by the phenol method.

[0081] Experiments show that the average contents of 20 samples are all within 90% of the theoretical contents. It shows that the desig...

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Abstract

The invention discloses a skin repair promoting peptide as well as a preparation method and application thereof, and relates to the technical field of polypeptide skin repair. The protein comprises a polypeptide with an amino acid sequence as shown in SEQ ID NO. 1. The polypeptide has the advantages of being good in dispersity, stable in acid-base environment, low in production cost and the like. Based on the rationality of the proportion design of hydrophobic amino acid, the skin repair promoting peptide can be uniformly dispersed in aqueous and lipid substances. The skin repair promoting peptide has relatively strong functions of promoting skin repair, promoting collagen expression, resisting oxidation and the like, and can be applied to the fields of preparation of medicines for body surface wound repair, burn repair, skin ulcer repair, skin repair for reducing scars and accelerating scar repair, preparation of regenerative beauty skin care products and cosmetics and the like.

Description

technical field [0001] The invention relates to the technical field of polypeptide skin repair, in particular to a skin repair-promoting peptide, a preparation method and an application thereof. Background technique [0002] Cosmetics currently on the market mainly contain two types: one is cosmetics with chemical raw materials as the main component, and the other is cosmetics with plant raw materials as the main component. Chemical cosmetics are quick to take effect, but contain toxic and harmful substances such as hormones, fruit acids, lead and mercury, and long-term use will cause greater damage to human skin; plant-based cosmetics are safer and healthier, but mainly replenish water from the skin surface , Supplement nutrients such as vitamins, and the nutrient elements cannot go deep into the inside of the cells, so there are problems of slow onset and ineffective effects. [0003] Compared with the above-mentioned cosmetics, cosmetics added with animal-derived growth ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/20A61K8/64A61K38/08A61Q19/00A61Q19/08A61P17/02A61P17/18
CPCC07K7/06A61K8/64A61K38/08A61Q19/00A61Q19/08A61P17/02A61P17/18A61K2800/10
Inventor 何阳
Owner 四川丽妍工坊生物科技有限公司
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