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Cell culture device and method thereof

A technology for cell culture and culture methods, applied in cell culture devices and its fields, capable of solving problems such as unresolved obstacles, achieving the effects of increasing cell adhesion, preventing harmful effects, and reducing waste accumulation

Pending Publication Date: 2021-11-30
CESCO BIOENGINEERING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] Several hurdles remain unresolved in cell culture technology

Method used

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  • Cell culture device and method thereof
  • Cell culture device and method thereof
  • Cell culture device and method thereof

Examples

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Embodiment Construction

[0057] The present invention provides an apparatus and method for culturing cells, and embodiments can be used to culture cells of different diversity, such as eukaryotic and prokaryotic cells, especially animal cells and / or mammalian cells. More particularly, anchorage-dependent animal cells.

[0058]The cell culture device of the present invention comprises a gas permeable chamber having at least one open end, a seal is removably mounted at the at least one open end, and a growth substrate is disposed in the gas permeable chamber, the seal being a lid or a substrate holder, And the growth substrate is a disk-shaped or ribbon-shaped porous substrate. Details of the cell culture devices in the various embodiments are described below.

[0059] figure 1 and figure 2 A side view of the cell culture apparatus of the first embodiment of the present invention is shown before and after assembly. The cell culture device includes a gas permeable chamber 101 , which is preferably a...

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Abstract

The invention provides a cell culture device and a method thereof. Devices for preparing and culturing cells are provided. The device comprises a gas permeable chamber having at least an inlet and an outlet for leading in and out a culture medium and cells, and a growth substrate placed at the end of the gas permeable chamber for anchoring or embedding cells. The invention also provides a simple and effective cell culture method. The cell culture device and the partially filled culture medium are slowly and horizontally rotated together, so that the cell culture device is horizontally rotated in the air-permeable chamber, the growth substrate can be intermittently exposed or immersed, and a better growth effect is obtained.

Description

technical field [0001] The present invention relates to devices and methods for growing animal cells and / or tissue cultures in vitro, and more particularly, the present invention relates to cell culture devices having gas permeable chambers and cell culture methods using the same. Background technique [0002] Over the years, cell culture methods have been widely developed for the growth of bacteria, yeasts and molds, all of which typically have strong cell walls and / or additional cellular material and are therefore more resilient. The structural elasticity of these microbial cells is a key factor contributing to the rapid development of efficient cell culture processes targeting these types of cells. For example, most bacterial cells can be grown in very large volumes of liquid medium using vigorous agitation, culture agitation, and gas sparging techniques to achieve good aeration during growth while maintaining viable cultures. [0003] In contrast, techniques for culturi...

Claims

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Application Information

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IPC IPC(8): C12M3/02C12M3/00C12M1/10
CPCC12M23/06C12M23/20C12M23/24C12M23/28C12M23/38C12M23/46C12M23/48C12M27/10C12M25/02C12M23/50C12M25/14
Inventor 黄楙砚张景明
Owner CESCO BIOENGINEERING CO LTD
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