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Preparation method of controllable hydrophobic bacterial cellulose-zein composite film

A technology of bacterial cellulose membrane and zein, which is applied in medical science, absorbent pads, surgery, etc., can solve the problems of high cost, no controllability, and limited surface modification effect of bacterial cellulose membrane, and achieve cost Low, enhanced cell adhesion properties, excellent mechanical properties and biocompatibility effects

Active Publication Date: 2016-01-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for preparing a controllable hydrophobic bacterial cellulose-zein composite film, which overcomes the problem of the prior art on the surface of the bacterial cellulose film. The modification effect is limited, it is not adjustable, and the cost is high.

Method used

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  • Preparation method of controllable hydrophobic bacterial cellulose-zein composite film
  • Preparation method of controllable hydrophobic bacterial cellulose-zein composite film
  • Preparation method of controllable hydrophobic bacterial cellulose-zein composite film

Examples

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Embodiment 1

[0035] Step 1, preparation of bacterial cellulose membrane

[0036] Medium composition: 2.0% D-glucose, 0.5% peptone, 0.5% yeast extract, 0.115% citric acid, 0.27% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH5.0.

[0037] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, and vibrate at 30°C for 12 hours, then inoculate 10% of the fermentation medium, shake well, and culture at 30°C for 5 days to obtain a bacterial cellulose film.

[0038] Step 2, purification of bacterial cellulose membrane

[0039] Take the bacterial cellulose membrane prepared above, wash it with water for 3 times, remove the culture medium, impurities and bacteria on the surface of the membrane; then soak the bacterial fiber wet membrane in a NaOH solution with a concentration of 0.2mol / L, at 90°C Take it out after boiling for 30 minutes, remove the culture medium and cells remaining in the film, then soak the film in distilled water, boil at 90°C for 30 minutes, c...

Embodiment 2

[0061] Step 1, preparation of bacterial cellulose membrane

[0062] Medium composition: 1.8% D-glucose, 0.45% peptone, 0.45% yeast extract, 0.120% citric acid, 0.25% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH4.8.

[0063] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 15%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.

[0064] Step 2, purification of bacterial cellulose membrane

[0065] Take the bacterial cellulose membrane prepared above, wash it with water for 4 times, soak it in NaOH solution with a concentration of 0.4mol / L, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, and put it under 90°C Boil for 30 minutes, change the water, repeat 4 times, then soak it in distilled water until neutral to obtain the purified bacterial cellulose membrane;

[0066] Step 3...

Embodiment 3

[0091] Step 1, preparation of bacterial cellulose membrane

[0092] Medium composition: 2.2% D-glucose, 0.6% peptone, 0.6% yeast extract, 0.110% citric acid, 0.30% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH4.0.

[0093] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 12%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.

[0094] Step 2, purification of bacterial cellulose membrane

[0095] Take the bacterial cellulose membrane prepared above, wash it with water for 5 times, soak it in 0.2mol / L KOH solution, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, boil it at 90°C for 30min, change the water , repeated 5 times, and then soaked in distilled water until neutral to obtain the purified bacterial cellulose membrane;

[0096] Step 3, pretreatment of bacterial cellulose m...

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Abstract

The invention discloses a preparation method of a controllable hydrophobic bacterial cellulose-zein composite film. The method comprises the following steps: firstly, preparing and purifying a bacterial cellulose film; then soaking the bacterial cellulose film into an ethanol-water solution to perform replacement treatment; then preparing a zein solution; reacting the bacterial cellulose film and the zein solution, and performing solvent evaporation after reaction ends to obtain a bacterial cellulose-zein composite wet film; and performing drying treatment to obtain the bacterial cellulose-zein composite film. The prepared bacterial cellulose-zein composite film disclosed by the invention has adjustable high hydrophobicity, excellent mechanical performance and biocompatibility, and can play roles of controlling and enhancing the adhesion capacity of cells, accelerating the tissue repair, and the like; and moreover, the preparation method disclosed by the invention is simple and convenient to operate, can be suitable for large-scale industrial production, and has relatively good application prospects in biomedical material industry.

Description

technical field [0001] The invention relates to a preparation method of a highly hydrophobic bacterial cellulose composite membrane material and the use of the composite membrane in tissue engineering repair materials, belonging to the related fields of biomedical materials and the like. Background technique [0002] Bacterial Cellulose (BC) is a microbial polysaccharide synthesized by fermentation of bacteria such as Acetobacter xylinum. It is a natural nanofiber polymer without branches composed of β-1,4 glycosidic bonds. A large number of studies have shown that BC has good in vivo and in vitro biocompatibility, biodegradability and shape controllability, so it is widely used in the field of biomedical materials. BC is suitable for scaffolds or repair materials in various tissue engineering, including skin, blood vessel, bone and cartilage tissue, etc., and has advantages in accelerating wound healing time and reducing infection rate. At present, corresponding products h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/44A61L15/32A61L15/28A61L15/42A61L31/04A61L31/16A61L31/14
Inventor 杨晓泉王丽英万芝力
Owner SOUTH CHINA UNIV OF TECH
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