Herbicide-resistant and insect-resistant transgenic soybean Jinjing bean 321 and specific detection method thereof

A transgenic soybean and herbicide-tolerant technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of poor resistance to lepidopteran pests, poor resistance of non-transgenic soybeans, etc., to improve tolerance Sexuality, the effect of enhancing resistance

Active Publication Date: 2021-11-30
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Non-GMO soybeans now have poor drug resis...

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  • Herbicide-resistant and insect-resistant transgenic soybean Jinjing bean 321 and specific detection method thereof
  • Herbicide-resistant and insect-resistant transgenic soybean Jinjing bean 321 and specific detection method thereof
  • Herbicide-resistant and insect-resistant transgenic soybean Jinjing bean 321 and specific detection method thereof

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[0029] The specific implementation manner of the present invention will be further described below in conjunction with the accompanying drawings. Wherein the same components are denoted by the same reference numerals.

[0030] In order to make the content of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention.

[0031] Such as Figure 1-7 As shown, a method for transgenic herbicide-resistant insect-resistant transgenic soybean Jingdou 321, the vector used for soybean transgenic transformation is pGR18, such as figure 1 Said, the vector has g2m-epsps, gr79m-epsps and cry1C genes, and the vector used has no screening marker gene and reporter gene except the target gene. The remaining selectable marker gene kanamycin resistance gene kanR for bacteria is not in the T-DNA, so it cannot be inserte...

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Abstract

The invention provides an herbicide-resistant and insect-resistant transgenic soybean Jinjing bean 321 and a specific detection method thereof. The herbicide-resistant and insect-resistant transgenic soybean Jinjing bean 321 is obtained by inserting an expression cassette consisting of glyphosate-resistant genes g2m-epsps and gr79m-epsps and an insect-resistant gene cry1C into a No.6 chromosome 9023775-902385 of a soybean genome by utilizing an agrobacterium-mediated method. The herbicide-resistant insect-resistant transgenic soybean can be further hybridized with an excellent soybean strain to improve and optimize other agronomic characters such as yield, quality and the like. A transformant specificity detection method is established by using primers derived from soybean genomes on two sides of a Jinjin bean 321 insertion site and primers derived from exogenous genes, a binding region of inserted T-DNA and plant genome DNA can be specifically identified, and seeds and other tissues of transgenic soybeans can be further identified.

Description

technical field [0001] The invention relates to the field of soybean transgenics, in particular to the herbicide-resistant insect-resistant transgenic soybean Jingdou 321 and a specific detection method thereof. Background technique [0002] Soybeans are native to China and are cultivated all over the world. The plant is 30-90 cm high, the stem is strong, erect, densely covered with brown long bristles, the leaves usually have 3 leaflets, the stipules are veined, covered with yellow pilose, and the petiole is 2-20 cm long. The total pedicel usually has 5-8 sessile, tightly squeezed flowers, the calyx is lanceolate, and the flowers are purple, lavender or white. Pods are hypertrophic, slightly curved, drooping, yellow-green, densely covered with brown-yellow long hairs; seeds 2-5, elliptic, subspherical. Non-GMO soybeans now have poor resistance to pesticides and poor resistance to Lepidoptera pests. Contents of the invention [0003] Aiming at the above-mentioned defici...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/54C12N15/32C12N15/11C12Q1/6895C12Q1/686A01H5/00A01H6/54
CPCC12N9/1092C12N15/8275C12N15/8286C07K14/325C12Q1/6895C12Q1/686C12Y205/01019C12Q2600/13C12Q2600/166C12Q2545/113C12Q2565/125Y02A40/146
Inventor 邱丽娟郭勇郭兵福张丽娟
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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