Method for evaluating developmental toxicity of high-concentration glucose by using zebra fish

Abstract

The invention discloses a method for evaluating developmental toxicity of high-concentration glucose by using zebra fish, which comprises the following steps: culturing zebra fish fertilized eggs in glucose culture solutions with different concentrations, counting the spine deformity rate and death rate of juvenile zebra fish by using glucose with different concentrations, observing the growth form of the zebra fish, and investigating the developmental toxicity of the high-concentration glucose in combination with the active oxygen generation amount, the cell apoptosis degree and the like of the juvenile fish. The method provided by the invention is simple and convenient to operate, short in evaluation period and capable of efficiently and intuitively evaluating the developmental toxicity of the high-concentration glucose by using the zebra fish, not only can evaluate the developmental toxicity of the high-concentration glucose on the zebra fish, but also can provide reference for the pathological mechanism research of the developmental toxicity of the high-concentration glucose on other organisms, especially human.

Description

technical field [0001] The invention relates to the technical field of toxicology detection, in particular to a method for evaluating the developmental toxicity of high-concentration glucose by using zebrafish. Background technique [0002] With the continuous enrichment of material life, the incidence of gestational diabetes has increased significantly, endangering the health of pregnant women and fetuses. Gestational diabetes has significantly increased the incidence of pregnancy-induced hypertension, premature birth, neonatal asphyxia, and macrosomia. High concentrations of glucose have neurotoxic effects, resulting in excessive oxygen free radicals and the production of glycation end products. These toxic side effects will cause great damage to the health of the mother and the early development of the fetus, and even directly cause developmental deformities or die. At present, the research on the pathological mechanism of these high-glucose side effects mostly uses youn...

AI Technical Summary

Problems solved by technology

High concentrations of glucose have neurotoxic effects, resulting in excessive oxygen free radicals and the production of glycation end products. These toxic side effects will cause great damage to the health of the mother and the early development of the fetus, and even directly cause developmental deformities or die

At present, the research on the pathological mechanism of these high-glucose side effects mostly uses young mice, rats, rabbits or cultured cells in vitro. Although this method is widely used, it is expensive, time-consuming, and complicated to operate, and the phenomenon is not easy to observe.

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