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Preparation method and thin-layer identification method of rhizoma dioscoreae septemlobae test sample

A thin-layer identification technology, which is applied in the field of preparation of the test sample of the scorpion, can solve the problems of long preparation time and complicated operation, achieve clear spots, improve the preparation efficiency, and reduce the effect of environmental pollution

Active Publication Date: 2021-12-07
BEIJING KANGRENTANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to overcome the problems of long preparation time and complicated operation of the test sample solution of the sponge worm in the prior art; thereby providing a preparation of the sponge worm test sample with high preparation efficiency and simple operation method, and discloses a method for thin-layer identification of the test solution prepared by the method

Method used

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  • Preparation method and thin-layer identification method of rhizoma dioscoreae septemlobae test sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A kind of thin-layer identification method of the cotton-leaf test product, concrete process is as follows:

[0058] 1. Preparation of the test solution:

[0059] Preparation of the test solution: get 0.5 g of the formula granules of Spongeonia sativa, add 25 ml of water, 2 ml of hydrochloric acid, heat and reflux for 15 minutes, let cool, shake and extract 2 times with ethyl acetate, 20 ml each time, combine the ethyl acetate liquid, evaporate Dry, add methanol 2ml to the residue to dissolve, as the test solution.

[0060] Preparation of the reference medicinal material solution: Take 0.5 g of the reference medicinal material of Spongelos chinensis, add 25 ml of water, 2 ml of hydrochloric acid, heat and reflux for 15 minutes, let it cool, shake and extract with ethyl acetate twice, 20 ml each time, combine the ethyl acetate solution, and evaporate to dryness , Add 2ml of methanol to the residue to dissolve it, and use it as the reference drug solution.

[0061] Prep...

Embodiment 2

[0065] The difference between this embodiment and Example 1 is that the sample volumes of the test solution used in the present invention and the reference medicinal material solution are different, and the specific settings are: the sample volumes of the reference medicinal material of S. 10μl, 15μl, the sample volume of the test solution is 1μl, 2μl, 3μl, 4μl, 5μl, see the results Figure 2-3 shown. Should figure 2 Among them, number 1 is the reference substance of diosgenin, and numbers 2-7 correspond to the sample volumes of 1 μl, 3 μl, 5 μl, 7 μl, 10 μl, and 15 μl of the reference medicinal material of Spongeonia spp. Should image 3 Among them, No. 1 is the reference substance of diosgenin, No. 2 is the control medicinal material of Spongeba sativa, and No. 3-7 correspond to the sample volume of 1 μl, 2 μl, 3 μl, 4 μl, and 5 μl of the test solution in turn.

[0066] It can be seen from the detection results that the method of the present invention can effectively rea...

Embodiment 3

[0068] This embodiment verifies the durability of the thin-layer identification in embodiment 1, specifically including:

[0069] 1. Investigation of different temperature and humidity

[0070] Prepare the reference substance solution, the reference medicinal material solution and the test solution according to the selected method, and according to their conditions, respectively, in normal temperature and humidity (T: 24.9 ° C, RH: 52%), low temperature and high humidity (T: 8.5 ° C, RH : 84%), normal temperature and low humidity (T: 24.9°C, RH: 14%), normal temperature and high humidity (T: 25.4°C, RH: 71%) conditions, take it out, dry it, develop color, and put it under a UV lamp ( 365nm), the results are shown in Figure 4-7 . Should Figure 4-7 No. 1 is the reference substance of diosgenin, No. 2 is the reference medicinal material of Mianzi, No. 3 is KL200520-322300-01 Mianzi formula granules, No. 4 is KL200520-437300-02 Mianzi formula granules, and No. 5 is KL200525-4...

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Abstract

The invention provides a preparation method and thin-layer identification method of a rhizoma dioscoreae septemlobae test sample. The rhizoma dioscoreae septemlobae test sample preparation method comprises the steps that a rhizoma dioscoreae septemlobae test sample is taken and mixed with a hydrochloric acid solution with the volume concentration being 5% to 10%, then extraction is conducted for 10 to 60 min, after extraction, an extraction solvent is adopted for extraction to obtain an extraction solution, and the extraction solution is combined and evaporated to dryness, and residues are dissolved by adding a dissolving solvent to obtain a test solution. The method has the advantages that the operation is simpler and more convenient, the preparation time is greatly saved, the preparation efficiency is improved, the method is safe and non-toxic, and the method is very friendly to detection personnel and the environment.

Description

technical field [0001] The invention relates to the field of thin-layer identification, in particular to a preparation method of a test sample of cotton sage and a thin-layer identification method thereof. Background technique [0002] The yam is the dried rhizome of Dioscorea spongiosa J.Q.Xi, M.Mizuno et W.L.Zhao or Fuzhou yam Dioscorea futschauensis Μline ex R.Kunth. This product is flat in taste and bitter in taste, and belongs to the kidney and stomach meridians. It is beneficial to dampness and turbidity, and has the effect of expelling wind and relieving numbness. Clinically, it is often used to treat ointment stranguria, cloudy, excessive leucorrhea, rheumatic arthralgia, joint discomfort, and pain in the waist and knees. [0003] The main components contained in the cotton vetch are steroidal saponins, lignans and diaryl heptanes and other compounds, in addition to steroid glycosides and so on. 2015, 2020 editions "Pharmacopoeia of the People's Republic of China"...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/90G01N30/94
CPCG01N30/06G01N30/90G01N30/94Y02A50/30
Inventor 张志强安琪史国华吉艳慧付静
Owner BEIJING KANGRENTANG PHARMA
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