Neural stem cell nose drop as well as preparation method and application thereof

A technology of neural stem cells and nasal drops, which is applied in the field of biomedicine, can solve the problems of insufficient resistance to nerve damage and limited repair ability of neural stem cells, and achieve the effect of convenient use

Inactive Publication Date: 2021-12-10
广州市新甡命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the repair ability of endogenous neural stem cells is limited, which is not enough to resist the nerve damage caused by ischemic stroke

Method used

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  • Neural stem cell nose drop as well as preparation method and application thereof
  • Neural stem cell nose drop as well as preparation method and application thereof
  • Neural stem cell nose drop as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: A neural stem cell nasal drop, including neural stem cell exosome freeze-dried powder, surfactant, stabilizer and osmotic pressure regulator;

[0035] The surfactant is lecithin;

[0036] The stabilizer is oleic acid;

[0037] The osmotic pressure regulator is glycerol.

[0038] In every 100ml of nasal drops, the lyophilized powder of exosomes of neural stem cells is 0.5-1g / ml, the surfactant is 10-20g / ml, the stabilizer is 30-40g / ml, and the osmotic The pressure regulator is 40-60g / ml.

[0039] The particle size of the nasal drops is 200-230nm.

[0040] In every 100ml of nasal drops, 5-10g / ml of preservatives can also be included.

[0041] The preservative is benzoic acid.

[0042] The preparation method of described a kind of neural stem cell nasal drop, comprises following process:

[0043] S1. Cultivate neural stem cells in a medium without exosome serum to obtain a culture supernatant containing exosomes from neural stem cells, centrifuge the cu...

Embodiment 2

[0058] Embodiment 2: Based on embodiment 1, in the S2 step of the preparation method of a kind of neural stem cell nasal drops, when performing ultrasonic mixing, the ultrasonic mixing and stirring equipment realizes, as image 3 As shown, the ultrasonic mixing and stirring equipment includes: an introduction device, a mixing container, an ultrasonic instrument, an analysis control device and an export device, the introduction device is connected to the mixing container, and the ultrasonic instrument includes a plurality of ultrasonic transceivers, and each ultrasonic transceiver is uniform Distributed around the outer wall of the mixing container. At the same time, the ultrasonic instrument is connected to the analysis control device, and the analysis control device is connected to the derivation device. The derivation device is connected to the mixing container according to the control information of the analysis control device. The ultrasonic mixing equipment is used to achie...

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Abstract

The invention discloses neural stem cell nose drops as well as a preparation method and application thereof. The neural stem cell nose drops comprise neural stem cell exosome freeze-dried powder, a surfactant, a stabilizer and an osmotic pressure regulator; the surfactant is lecithin; the stabilizer is oleic acid; and the osmotic pressure regulator is glycerol. According to the neural stem cell nose drops as well as the preparation method and application thereof, endogenous neural stem cells of an organism are mobilized to treat cranial nerve injuries, including Alzheimer's disease, Parkinson's disease and depression.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a neural stem cell nasal drop. Background technique [0002] Brain injury and neurodegenerative diseases mainly include cerebral hemorrhage / ischemic injury, stroke, ischemic dementia, Alzheimer's disease, Parkinson's disease and traumatic brain injury, etc., with high disability rate, high mortality rate and high recurrence rate and many complications. Early post-brain injury is usually accompanied by symptoms such as loss of neurons in the brain, decline in consciousness, language, limb function, and learning and memory abilities. The sequelae period is mainly characterized by a stable condition, and the lost function is no longer significantly improved. [0003] Neural stem cells are a group of relatively primitive cells capable of self-renewal and multiple differentiation potentials, which can differentiate into neurons, oligodendrocytes and astrocytes. For example, end...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61K9/00A61K47/24A61K47/12A61K47/10A61P25/28A61P25/16A61P25/24C12N5/0797
CPCA61K35/30A61K9/0043A61K47/24A61K47/12A61K47/10A61P25/28A61P25/16A61P25/24C12N5/0623C12N2509/00
Inventor 顾军顾帅兰丹
Owner 广州市新甡命科技有限公司
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