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Method for promoting skin wound repair by neuregulin 1 and application of neuregulin 1

A skin wound and protein technology, applied in the fields of molecular biology and biomedicine, can solve problems such as no effective application of treatment

Active Publication Date: 2021-12-10
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above evidence shows that peripheral nerves can mediate the signal transduction between nerves and skin wound repair by secreting neurotransmitters and cytokines, but the relevant treatments based on these studies have not been effectively applied in clinical practice

Method used

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  • Method for promoting skin wound repair by neuregulin 1 and application of neuregulin 1
  • Method for promoting skin wound repair by neuregulin 1 and application of neuregulin 1
  • Method for promoting skin wound repair by neuregulin 1 and application of neuregulin 1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Changes of Neuregulin-1 / ErbB4 signaling pathway in skin wound repair

[0049] 1. Experimental materials

[0050] Experimental animals: C57BL / 6 mice used in the present invention were purchased from Weitong Lihua Company (experimental animal production license number: SCXK Beijing 2015-001) and Guangxi Medical University Animal Center (experimental animal production license number SCXK Gui 2003-001) 0003), various transgenic mice were also used for skin wound healing experiments, including ErbB4 knockout mice (ErbB4+ / -), various conditional gene knockout mice such as Krt14-Cre; ErbB- / -, ErbB4- Floxed; Nestin-CreER; Nestin-GFP mice were used for experiments. Among them, ErbB4 knockout mice (ErbB4+ / -) came from the laboratory of Professor Martin Gassmann at the University of Basel, Switzerland, ErbB4-Flox mice came from the laboratory of Professor Cary Lai at Indiana University, USA, and Krt14-Cre mice were purchased from Nanfang, Shanghai Model Biotechnology C...

Embodiment 2

[0070] Example 2 The role of Neuregulin-1 / ErbB4 signaling pathway in skin wound repair

[0071] 1 Experimental materials

[0072] 1.1 Experimental animals: The source of C57BL / 6 mice is the same as in Example 1. We use ErbB4+ / - for mating and breeding, and carry out genetic identification on their offspring. Because ErbB4 knockout homozygotes are likely to cause lethal heart dysplasia embryos, it is difficult to obtain, so In this experiment, ErbB4+ / - was used to observe wound healing, ErbB4+ / - mice were used as the experimental group and wild-type mice were used as the control group. According to the relevant ethical rules of the Experimental Animal Center of Guangxi Medical University, every effort has been made to reduce the harm to animals in the experiments.

[0073] 2 Experimental methods

[0074] 2.1 ErbB4 receptor inhibition pharmacology experiment

[0075] 2.1.1 Validation experiment of the effect of AG1478 (500 μm) on ErbB4 activity at the wound margin: 1) Hair re...

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Abstract

The invention discloses an application of NRG1 and a receptor thereof to preparation of a skin wound repair medicine or reagent. The NRG1 promotes the skin wound repair by promoting phosphorylation of ErbB4. The invention also discloses a pharmaceutical composition for promoting skin wound repair. The pharmaceutical composition is a carrier containing an NRG1 gene. In addition, the invention also provides a method for screening potential substances for promoting skin wound repair. The NRG1 and the pharmaceutical composition thereof disclosed by the invention have a relatively good promotion effect on skin wound repair, and have important reference and practical significance in skin wound repair. In addition, the method for screening the potential substances for promoting skin wound repair, provided by the invention, has a good effect on screening medicines and substances for skin wound repair, and has better measuring and guiding effects on screening proper substances for skin wound repair.

Description

technical field [0001] The invention relates to a protein related to skin wound repair and its application in promoting skin wound repair, specifically the protein is NRG1 and its receptor. The invention belongs to the field of biomedicine, specifically to the technical field of molecular biology. Background technique [0002] The skin is the largest sensory organ in the human body, with functions such as barrier, sensation, immune thermoregulation, and endocrine excretion. Large-scale skin defects caused by various acute injuries such as burns, skin avulsions, and surgical operations are very common. In addition, the incidence of refractory skin wounds caused by chronic injury factors, such as pressure ulcers, venous ulcers, and diabetic ulcers, is also increasing year by year, which seriously affects the economy and quality of life of patients, and brings great pressure to wound repair surgeons. Come to great challenge. The process of skin wound repair is a complex and o...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K48/00A61K38/18A61P17/02C12Q1/6883G01N33/68
CPCA61K45/00A61K48/0008A61K48/005A61K38/1883A61P17/02C12Q1/6883G01N33/74C12Q2600/158C12Q2600/136G01N2333/4756G01N2800/52G01N2800/20G01N2500/02
Inventor 谭国鹤刘媛媛李顺堂赵劲民伍伟锋陈相任
Owner GUANGXI MEDICAL UNIVERSITY
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