Mutant of pore protein monomer, protein pore and application thereof
A porin and mutant technology, applied in the field of characterization of target analyte characteristics, can solve the problem of single nanoporin
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Embodiment 1
[0225]In an embodiment, the wild-type porin is from Gamma Proteus, and the amino acid sequence of the wild-type porin is SEQ ID NO:1, and the nucleotide sequence encoding the amino acid sequence is shown in SEQ ID NO:2. Mutant 1 of the porin monomer is that the wild-type porin has multiple mutations corresponding to SEQ ID NO: 1, specifically Q64L, A71L, T75A, and F76N. The protein pore of mutant 1 that includes a porin monomer is mutant pore 1. The amino acid sequence of mutant 1 of the protein monomer is shown in SEQ ID NO:16.
Embodiment 2
[0227] In an embodiment, the wild-type porin is from Gamma Proteus, and the amino acid sequence of the wild-type porin is SEQ ID NO:1, and the nucleotide sequence of the sequence encoding this amino acid is shown in SEQ ID NO:2. Mutant 2 of the porin monomer is that the wild-type porin has multiple mutations corresponding to SEQ ID NO: 1, specifically T75S and F76N. The protein pore of mutant 2 that includes a porin monomer is mutant pore 2. The amino acid sequence of mutant 2 of the protein monomer is shown in SEQ ID NO:17.
Embodiment 3
[0229] In an embodiment, the wild-type porin is from Gamma Proteus, and the amino acid sequence of the wild-type porin is SEQ ID NO:1, and the nucleotide sequence of the sequence encoding this amino acid is shown in SEQ ID NO:2. Mutant 3 of the porin monomer is that the wild-type porin has multiple mutations corresponding to SEQ ID NO: 1, specifically A71L, T75S, and F76Q. The protein pore of mutant 3 that includes a porin monomer is mutant pore 3. The amino acid sequence of mutant 3 of the protein monomer is shown in SEQ ID NO:18.
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