30 results about "Pore Proteins" patented technology

The present invention provides compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccarhide at a concentration of no greater than about 10.0 endotoxin units per milliliter. The present invention also provides methods of making and methods of using such compositions.

The invention discloses eel aeromonas hydrophila and edwardsiella tarda bigeminal recombinant protein and a preparation method thereof. The bigeminal recombinant protein disclosed by the invention comprises an aeromonas hydrophila type II pore protein membrane outer part and an edwardsiella tarda ompS2 membrane outer part which are connected together, and the amino acid sequence of the bigeminal recombinant protein is as shown by SEQ ID NO1. According to the preparation method disclosed by the invention, the aeromonas hydrophila type II pore protein membrane outer part and the edwardsiella tarda ompS2 membrane outer part are connected together through a genetic engineering means to construct the bigeminal recombinant protein, and the bigeminal recombinant protein can prevent inflections of aeromonas hydrophila and edwardsiella tarda at the same time after being used for immunizing eels, and has a better immune effect compared with immunization with single outer membrane protein. The bigeminal recombinant protein disclosed by the invention can obtain an immune effect on a variety of pathogenic bacteria through only once injection on a fish body, so that injuries to the fish body are reduced, and immunization steps are simplified; the preparation method of the bigeminal recombinant protein is suitable for industrial production.

The invention discloses a preparation method of a calixarene modified magnetic porous protein gel adsorbent. The preparation method is characterized in that a nano ferroferric oxide and xanthan gum mixture is added to collagen powder for magnetization, and magnetic porous protein gel microspheres are prepared with succinaldehyde as a crosslinking agent; the magnetic porous protein gel microspheresare pretreated with ethylenediamine and ammonium hydroxide, and pretreated magnetic porous protein gel microspheres are prepared; then, 72%-76% of trichloromethane, 10%-14% of thionyl chloride and 6%-8% of 4-tert-butyl calix[4]arene are added to a reactor in percentage by mass, the mixture is stirred for dissolution, 6%-8% of the pretreated magnetic porous protein gel microspheres are added, themixture reacts for 4 h, solid-liquid separation and drying are performed, and the calixarene modified magnetic porous protein gel adsorbent is obtained. The adsorbent has very high adsorption capacityfor thorium ions, specificity, low cost and high mechanical strength, can be reused for 10 times or more and is easy to separate.

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO:1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or more positions corresponding to Q68, N72 and F73 of SEQ ID NO:1.

The invention relates to a method for preparing a nano-palladium catalyst by utilizing a protein assembly, which is characterized in that the nano-palladium catalyst with controllable particle size and high dispersity is prepared by utilizing a porous protein assembly, taking protein as a reducing agent and taking the pores of the assembly as a reactor. In the prior art, the preparation method of the metal nano-catalyst mainly has the problems that the preparation process is not environment-friendly, the energy consumption is high, the cost is high, the particle size is uncontrollable, the dispersity is poor and the like. The problem can be solved by the method for preparing the nano-palladium catalyst by using the protein assembly. The invention has the main advantages that: 1) the protein assembly has no environmental pollution problem in preparation and reaction processes; 2) the particle size of the nano-palladium is limited by the pore size of the protein assembly, so that the particle size can be controlled; and (3) the synthesized nano-palladium catalyst is good in dispersity and high in catalytic efficiency.

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte; wherein the amino acids of the mutant of the pore protein monomer comprise mutations at one or more positions corresponding to A74, P75, G76, N77, A78, T79, N80 and F81 of SEQ ID No: 1.

Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins, which may be utilized in vaccines, including anti- bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. Suitable carriers may include but are not limited to Haemophilus influenzae protein D (PD), Neisseria meningitidis porin protein (PorA), Corynebacterium diphtheriae toxin (CRM 197), Clostridium tetani toxin (TT), and Escherichia coli maltose binding protein, and variants thereof.

The invention relates to a preparation method of stable and uniform nanopores, which comprises the following steps: mixing and incubating a nanopore monomer and a lipid vesicle physiological buffer turbid liquid with uniform particle size, and forming a uniform and stable nanopore precursor on lipid vesicles; sequentially adding an unlocking agent to enable the nanopore precursor on the lipid vesicles to form nanopores, adding a cross-linking agent to enable the formed nanopore structure to be more stable, and adding a surfactant to destroy the lipid vesicles and enable the nanopores to enter the aqueous solution to form a mixed solution of the nanopores and lipid; and separating and purifying to obtain the nanopores with consistent molecular weight, uniform pore diameter and stable structure. The pore-forming protein obtained by the invention can form a pre-pore-forming compound on specially-made lipid vesicles, and then a cross-linking agent and a surfactant are combined, so that the structure of a nanopore is more stable, and the pore-forming success rate is higher.

The invention relates to a biomask and a method for patterning on a substrate, and belongs to the technical field of integrated circuit preparation. A method of patterning on a substrate includes the steps: forming a photoresist layer on the substrate; forming a biological mask on the surface, deviating from the substrate, of the photoresist layer; exposing and developing the biological mask, removing the biological mask, etching, and removing the photoresist to obtain the patterned substrate, wherein the biomask comprises a phospholipid double-layer membrane and pore protein embedded in the phospholipid double-layer membrane. In the application, the mask used when patterning is carried out on the substrate is the biological mask, and the pattern is formed through the pore protein, so the process cost can be effectively reduced.

The invention provides a method for improving the stability of a nano material. Flagellin FilC and pore protein OmpF from Rahnella aquatilis and Escherichia coli are used for improving the stability of the nano-material, and the nano-material can be selected from nano-selenium, nano-gold and nano-silver. Compared with other nano-material stabilizers (such as SDS, PEG, PVP, BSA and escherichia coli FilC and OmpF), the stability efficiency of the required Rahnella aquatica FilC and OmpF is 10-100 times that of PVP, BSA and escherichia coli FilC and OmpF under the condition that stable chemical synthesis of nano-selenium is not affected by strong acid, strong alkali and strong ion concentration. The rahnella aquatica FliC and OmpF can also significantly improve the stability of the nano-gold and nano-silver under the condition of strong ion concentration, and the effect is significantly superior to that of escherichia coli FilC and OmpF.

The invention provides a neisseria gonorrhoeae-salmonella double-effect vaccine, and a preparation method and an application thereof, wherein a salmonella ghost is used as a delivery vector for loading a neisseria gonorrhoeae PorB DNA vaccine. The preparation method mainly includes the following steps: 1) with a neisseria gonorrhoeae genome as a template, carrying out PCR amplification to obtain a porin (PorB) gene and constructing a PorB DNA vaccine; 2) constructing a temperature control expression vector pB-E, transforming into a salmonella competent state to obtain recombinant bacteria SE (pB-E), and carrying out temperature induction to prepare a salmonella ghost vaccine freeze-dried powder; and 3) incubating the neisseria gonorrhoeae PorB DNA vaccine with the salmonella ghost freeze-dried powder, to obtain the salmonella ghost-loaded neisseria gonorrhoeae PorB bacterial ghost vaccine SE ghost (PorB). The double-effect vaccine has good safety, can also induce a body to generate and increase specific humoral immune responses and mucosal immune responses on neisseria gonorrhoeae and salmonella, has relatively high antibody level, and is of great significance in prevention and treatment of gonorrhea and/or salmonella.

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO: 1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or more positions corresponding to S79, S80 and F81 of SEQ ID NO: 1.

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant, and an application of the mutant or the protein pore to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO: 1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or more positions corresponding to Y76, S79, S83 and F84 of SEQ ID NO: 1.

The invention discloses a method for constructing double-recognition-site nanopores, which comprises the following steps of carrying out structural domain function analysis on two single-recognition-site nanopores subjected to fusion expression to obtain a structural domain analysis result, selecting a structural domain gene, and carrying out fusion protein design to obtain a fusion protein with two recognition sites, selecting an expression system capable of ensuring the stable and polymerizable structure of the fusion protein, and sequentially finishing expression scheme design, expression condition optimization, pore protein polymerization, purification and separation, pore embedding and sequencing and fusion protein mutation treatment according to the host type of the expression system. The naturally existing nanopore protein with the single recognition site is used, and the nanopore protein with the double recognition sites is obtained through an engineered fusion protein expression technology, so that the selectable range of the nanopore protein is greatly expanded, and the problem that the naturally existing protein nanopores with the double sensitive sites are extremely fewis solved. By mutating and optimizing the performance of the pore channels, the detection performance of the pore channels is improved.

The invention provides a method for improving the stability of a nano material. Flagellin FilC and pore protein OmpF from Rahnella aquatilis and Escherichia coli are used for improving the stability of the nano-material, and the nano-material can be selected from nano-selenium, nano-gold and nano-silver. Compared with other nano-material stabilizers (such as SDS, PEG, PVP, BSA and escherichia coli FilC and OmpF), the stability efficiency of the required Rahnella aquatica FilC and OmpF is 10-100 times that of PVP, BSA and escherichia coli FilC and OmpF under the condition that stable chemical synthesis of nano-selenium is not affected by strong acid, strong alkali and strong ion concentration. The rahnella aquatica FliC and OmpF can also significantly improve the stability of the nano-gold and nano-silver under the condition of strong ion concentration, and the effect is significantly superior to that of escherichia coli FilC and OmpF.

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO: 1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or two positions corresponding to T75 and F76 of SEQ ID NO: 1.

The invention relates to a method for preparing a gold-palladium nano-alloy by using a protein assembly, which is characterized in that palladium ions and gold ions in palladium salt and chloroauric acid are co-reduced to form the gold-palladium nano-alloy by using a three-dimensional porous protein assembly. In the prior art, the preparation method of the metal nano-alloy catalyst mainly has the problems that the preparation process is not environment-friendly, the energy consumption is high, the cost is high and the like. The problem can be solved by using the protein assembly to prepare the nano-palladium catalyst. The method has the main advantages that 1) the preparation process is environment-friendly and pollution-free; 2) the cost is low, and the energy consumption is low; and (3) the prepared gold-palladium nano-alloy compound is good in dispersity and does not agglomerate.

The invention relates to a method for increasing the coproduced fermentation yield of S-ademetionine and glutathione. According to the method, the gene knockout technology is adopted for knocking out membrane pore protein genes on a mitochondrial membrane of candida utilis, the permeability of the mitochondrial membrane is improved, entry of NADH into mitochondria is promoted, the efficiency of synthesizing ATP through a respiratory chain is improved, and finally the coproduced yield of SAM and GSH in yeast cells is increased. The method provides a new thought for a high yield of similar energy-consuming aerobiotic synthetic compounds in eukaryocytes.