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33 results about "Pore Proteins" patented technology

Definitions - pore proteins. Pore Proteins (n.) 1.(MeSH)Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules.

Immunizing compositions and methods of use

InactiveUS20060165718A1High somatic cell countLow milk productionAntibacterial agentsBiocideMicroorganismToxin
The present invention provides compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccarhide at a concentration of no greater than about 10.0 endotoxin units per milliliter. The present invention also provides methods of making and methods of using such compositions.
Owner:EPITOPIX LLC

Eel aeromonas hydrophila and edwardsiella tarda bigeminal recombinant protein and preparation method thereof

The invention discloses eel aeromonas hydrophila and edwardsiella tarda bigeminal recombinant protein and a preparation method thereof. The bigeminal recombinant protein disclosed by the invention comprises an aeromonas hydrophila type II pore protein membrane outer part and an edwardsiella tarda ompS2 membrane outer part which are connected together, and the amino acid sequence of the bigeminal recombinant protein is as shown by SEQ ID NO1. According to the preparation method disclosed by the invention, the aeromonas hydrophila type II pore protein membrane outer part and the edwardsiella tarda ompS2 membrane outer part are connected together through a genetic engineering means to construct the bigeminal recombinant protein, and the bigeminal recombinant protein can prevent inflections of aeromonas hydrophila and edwardsiella tarda at the same time after being used for immunizing eels, and has a better immune effect compared with immunization with single outer membrane protein. The bigeminal recombinant protein disclosed by the invention can obtain an immune effect on a variety of pathogenic bacteria through only once injection on a fish body, so that injuries to the fish body are reduced, and immunization steps are simplified; the preparation method of the bigeminal recombinant protein is suitable for industrial production.
Owner:JIMEI UNIV

Strain GX-3 and method for recycling nano gold from gold-ion-bearing wastewater

The invention relates to strain GX-3, in particular to strain GX-3 for efficiently recycling noble metal ions from wastewater, such as mine wastewater, electronic wastewater, and belongs to the technical field of environmental microorganisms. The strain GX-3 has a taxonomic name, Delftia tsuruhatensis GX-3 and is also a sulfur-reducing bacterium. The strain gauge GX-3 is collected in CGMCC (China General Microbiological Culture Collection Center) under CGMCC No. 13430 on December 07, 2016. Au3+ is added to sulfur-reducing strain GX-3 culture liquid, cell membrane pore protein of the strain GX-3 is reduced directly into Au3+ as elemental Au0; the elemental Au0 is combined with the pore protein to form nano gold particles, and precipitation in the solution is avoided. High-speed centrifugal method is used to separate and collect nano gold particles, and the concentration of residue Au3+ in the solution is detected via ICP-MS analysis.
Owner:NINGBO URBAN ENVIRONMENT OBSERVATION & RES STATION NUEORS CHINESE ACADEMY OF SCI +1

Artificially designed pore-forming proteins with anti-tumor effects

InactiveUS20050256040A1Limited anti-tumor effectModerate efficacyPeptide/protein ingredientsAntineoplastic agentsAbnormal tissue growthCell membrane
Protein engineering is an emerging area that has expanded the understanding in the art of protein folding and laid the groundwork for the creation of unprecedented structures with unique functions. The first native-like pore-forming protein, small globular protein (SGP), has previously been designed. It has now been discovered that this artificially engineered protein, and analogs and homologs thereof, have membrane-disrupting properties and anti-tumor activity in several cancer animal models. A mechanism for the selectivity of SGP toward cell membranes in tumors is proposed and validated herein, thereby confirming the proposed mechanism of action. Thus, SGP is established herein as the prototype for a new class of artificial proteins designed for therapeutic applications.
Owner:THE BUCK INST FOR RES ON AGING

Preparation method of calixarene modified magnetic porous protein gel adsorbent

The invention discloses a preparation method of a calixarene modified magnetic porous protein gel adsorbent. The preparation method is characterized in that a nano ferroferric oxide and xanthan gum mixture is added to collagen powder for magnetization, and magnetic porous protein gel microspheres are prepared with succinaldehyde as a crosslinking agent; the magnetic porous protein gel microspheresare pretreated with ethylenediamine and ammonium hydroxide, and pretreated magnetic porous protein gel microspheres are prepared; then, 72%-76% of trichloromethane, 10%-14% of thionyl chloride and 6%-8% of 4-tert-butyl calix[4]arene are added to a reactor in percentage by mass, the mixture is stirred for dissolution, 6%-8% of the pretreated magnetic porous protein gel microspheres are added, themixture reacts for 4 h, solid-liquid separation and drying are performed, and the calixarene modified magnetic porous protein gel adsorbent is obtained. The adsorbent has very high adsorption capacityfor thorium ions, specificity, low cost and high mechanical strength, can be reused for 10 times or more and is easy to separate.
Owner:UNIV OF JINAN

Therapeutic pore-forming peptides

A class of procytotoxic agents is characterized by a capability to kill with target cell-specificity. Such an aspect can be a pore-forming protein which has at least one lysine residue, modified by a peptide linkage to an amino acid residue, via the epsilon amino group. These agents are useful in treating cancer, especially prostate cancer.
Owner:ORBIS HEALTH SOLUTIONS

Mutant of pore protein monomer, protein pore and application thereof

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO:1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or more positions corresponding to Q68, N72 and F73 of SEQ ID NO:1.
Owner:QITAN TECH LTD CHENGDU

Method for preparing nano-palladium catalyst by using protein assembly

The invention relates to a method for preparing a nano-palladium catalyst by utilizing a protein assembly, which is characterized in that the nano-palladium catalyst with controllable particle size and high dispersity is prepared by utilizing a porous protein assembly, taking protein as a reducing agent and taking the pores of the assembly as a reactor. In the prior art, the preparation method of the metal nano-catalyst mainly has the problems that the preparation process is not environment-friendly, the energy consumption is high, the cost is high, the particle size is uncontrollable, the dispersity is poor and the like. The problem can be solved by the method for preparing the nano-palladium catalyst by using the protein assembly. The invention has the main advantages that: 1) the protein assembly has no environmental pollution problem in preparation and reaction processes; 2) the particle size of the nano-palladium is limited by the pore size of the protein assembly, so that the particle size can be controlled; and (3) the synthesized nano-palladium catalyst is good in dispersity and high in catalytic efficiency.
Owner:NORTHWESTERN POLYTECHNICAL UNIV

Scaffold

A method of preparing a porous protein scaffold for supporting the growth of biological tissue is described. The method comprises: providing an oil-in water emulsion comprising oil droplets dispersed in a continuous phase comprising a pH-buffered aqueous protein solution, wherein the oil-in-water emulsion comprises a non-ionic surfactant in an amount of 0.01 to 10 volume % of the total volume of the oil phase in the oil-in-water emulsion; gelling the protein around the oil droplets, such as by enzymatic activity or by non-enzymatic activity chemical reaction or by thermally controlled gelation; and removing the oil droplets from the continuous phase. A porous protein scaffold and its uses are also described.
Owner:OXFORD UNIV INNOVATION LTD

Mutant of pore protein monomer, protein pore and application thereof

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte; wherein the amino acids of the mutant of the pore protein monomer comprise mutations at one or more positions corresponding to A74, P75, G76, N77, A78, T79, N80 and F81 of SEQ ID No: 1.
Owner:QITAN TECH LTD CHENGDU

Bioconjugate vaccines' synthesis in prokaryotic cell lysates

Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins, which may be utilized in vaccines, including anti- bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. Suitable carriers may include but are not limited to Haemophilus influenzae protein D (PD), Neisseria meningitidis porin protein (PorA), Corynebacterium diphtheriae toxin (CRM 197), Clostridium tetani toxin (TT), and Escherichia coli maltose binding protein, and variants thereof.
Owner:NORTHWEST UNIV +1

Preparation method of stable and uniform nanopore

The invention relates to a preparation method of stable and uniform nanopores, which comprises the following steps: mixing and incubating a nanopore monomer and a lipid vesicle physiological buffer turbid liquid with uniform particle size, and forming a uniform and stable nanopore precursor on lipid vesicles; sequentially adding an unlocking agent to enable the nanopore precursor on the lipid vesicles to form nanopores, adding a cross-linking agent to enable the formed nanopore structure to be more stable, and adding a surfactant to destroy the lipid vesicles and enable the nanopores to enter the aqueous solution to form a mixed solution of the nanopores and lipid; and separating and purifying to obtain the nanopores with consistent molecular weight, uniform pore diameter and stable structure. The pore-forming protein obtained by the invention can form a pre-pore-forming compound on specially-made lipid vesicles, and then a cross-linking agent and a surfactant are combined, so that the structure of a nanopore is more stable, and the pore-forming success rate is higher.
Owner:SHANGHAI JIAO TONG UNIV

Biomask and method for patterning on substrate

The invention relates to a biomask and a method for patterning on a substrate, and belongs to the technical field of integrated circuit preparation. A method of patterning on a substrate includes the steps: forming a photoresist layer on the substrate; forming a biological mask on the surface, deviating from the substrate, of the photoresist layer; exposing and developing the biological mask, removing the biological mask, etching, and removing the photoresist to obtain the patterned substrate, wherein the biomask comprises a phospholipid double-layer membrane and pore protein embedded in the phospholipid double-layer membrane. In the application, the mask used when patterning is carried out on the substrate is the biological mask, and the pattern is formed through the pore protein, so the process cost can be effectively reduced.
Owner:SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA

A method for increasing the co-production fermentation yield of s-adenosylmethionine and glutathione

The invention relates to a method for increasing the coproduced fermentation yield of S-ademetionine and glutathione. According to the method, the gene knockout technology is adopted for knocking out membrane pore protein genes on a mitochondrial membrane of candida utilis, the permeability of the mitochondrial membrane is improved, entry of NADH into mitochondria is promoted, the efficiency of synthesizing ATP through a respiratory chain is improved, and finally the coproduced yield of SAM and GSH in yeast cells is increased. The method provides a new thought for a high yield of similar energy-consuming aerobiotic synthetic compounds in eukaryocytes.
Owner:SUZHOU UNIV

Method for improving stability of nano material

The invention provides a method for improving the stability of a nano material. Flagellin FilC and pore protein OmpF from Rahnella aquatilis and Escherichia coli are used for improving the stability of the nano-material, and the nano-material can be selected from nano-selenium, nano-gold and nano-silver. Compared with other nano-material stabilizers (such as SDS, PEG, PVP, BSA and escherichia coli FilC and OmpF), the stability efficiency of the required Rahnella aquatica FilC and OmpF is 10-100 times that of PVP, BSA and escherichia coli FilC and OmpF under the condition that stable chemical synthesis of nano-selenium is not affected by strong acid, strong alkali and strong ion concentration. The rahnella aquatica FliC and OmpF can also significantly improve the stability of the nano-gold and nano-silver under the condition of strong ion concentration, and the effect is significantly superior to that of escherichia coli FilC and OmpF.
Owner:CHINA AGRI UNIV

A double recombinant protein of Aeromonas hydrophila and Edwardsiella tarda and its preparation method

The invention discloses eel aeromonas hydrophila and edwardsiella tarda bigeminal recombinant protein and a preparation method thereof. The bigeminal recombinant protein disclosed by the invention comprises an aeromonas hydrophila type II pore protein membrane outer part and an edwardsiella tarda ompS2 membrane outer part which are connected together, and the amino acid sequence of the bigeminal recombinant protein is as shown by SEQ ID NO1. According to the preparation method disclosed by the invention, the aeromonas hydrophila type II pore protein membrane outer part and the edwardsiella tarda ompS2 membrane outer part are connected together through a genetic engineering means to construct the bigeminal recombinant protein, and the bigeminal recombinant protein can prevent inflections of aeromonas hydrophila and edwardsiella tarda at the same time after being used for immunizing eels, and has a better immune effect compared with immunization with single outer membrane protein. The bigeminal recombinant protein disclosed by the invention can obtain an immune effect on a variety of pathogenic bacteria through only once injection on a fish body, so that injuries to the fish body are reduced, and immunization steps are simplified; the preparation method of the bigeminal recombinant protein is suitable for industrial production.
Owner:JIMEI UNIV

Neisseria gonorrhoeae-salmonella double-effect vaccine, and preparation method and application thereof

The invention provides a neisseria gonorrhoeae-salmonella double-effect vaccine, and a preparation method and an application thereof, wherein a salmonella ghost is used as a delivery vector for loading a neisseria gonorrhoeae PorB DNA vaccine. The preparation method mainly includes the following steps: 1) with a neisseria gonorrhoeae genome as a template, carrying out PCR amplification to obtain a porin (PorB) gene and constructing a PorB DNA vaccine; 2) constructing a temperature control expression vector pB-E, transforming into a salmonella competent state to obtain recombinant bacteria SE (pB-E), and carrying out temperature induction to prepare a salmonella ghost vaccine freeze-dried powder; and 3) incubating the neisseria gonorrhoeae PorB DNA vaccine with the salmonella ghost freeze-dried powder, to obtain the salmonella ghost-loaded neisseria gonorrhoeae PorB bacterial ghost vaccine SE ghost (PorB). The double-effect vaccine has good safety, can also induce a body to generate and increase specific humoral immune responses and mucosal immune responses on neisseria gonorrhoeae and salmonella, has relatively high antibody level, and is of great significance in prevention and treatment of gonorrhea and / or salmonella.
Owner:YANGZHOU UNIV

Mutant of pore protein monomer, protein pore and application of mutant

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO: 1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or more positions corresponding to S79, S80 and F81 of SEQ ID NO: 1.
Owner:QITAN TECH LTD CHENGDU

Mutant of pore protein monomer, protein pore, application of mutant and application of protein pore

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant, and an application of the mutant or the protein pore to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO: 1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or more positions corresponding to Y76, S79, S83 and F84 of SEQ ID NO: 1.
Owner:QITAN TECH LTD CHENGDU

Method for constructing double-recognition-site nanopores

The invention discloses a method for constructing double-recognition-site nanopores, which comprises the following steps of carrying out structural domain function analysis on two single-recognition-site nanopores subjected to fusion expression to obtain a structural domain analysis result, selecting a structural domain gene, and carrying out fusion protein design to obtain a fusion protein with two recognition sites, selecting an expression system capable of ensuring the stable and polymerizable structure of the fusion protein, and sequentially finishing expression scheme design, expression condition optimization, pore protein polymerization, purification and separation, pore embedding and sequencing and fusion protein mutation treatment according to the host type of the expression system. The naturally existing nanopore protein with the single recognition site is used, and the nanopore protein with the double recognition sites is obtained through an engineered fusion protein expression technology, so that the selectable range of the nanopore protein is greatly expanded, and the problem that the naturally existing protein nanopores with the double sensitive sites are extremely fewis solved. By mutating and optimizing the performance of the pore channels, the detection performance of the pore channels is improved.
Owner:SHENZHEN MERRIME NANOPORE TECH CO LTD

Method for improving stability of nano material

The invention provides a method for improving the stability of a nano material. Flagellin FilC and pore protein OmpF from Rahnella aquatilis and Escherichia coli are used for improving the stability of the nano-material, and the nano-material can be selected from nano-selenium, nano-gold and nano-silver. Compared with other nano-material stabilizers (such as SDS, PEG, PVP, BSA and escherichia coli FilC and OmpF), the stability efficiency of the required Rahnella aquatica FilC and OmpF is 10-100 times that of PVP, BSA and escherichia coli FilC and OmpF under the condition that stable chemical synthesis of nano-selenium is not affected by strong acid, strong alkali and strong ion concentration. The rahnella aquatica FliC and OmpF can also significantly improve the stability of the nano-gold and nano-silver under the condition of strong ion concentration, and the effect is significantly superior to that of escherichia coli FilC and OmpF.
Owner:CHINA AGRI UNIV

A method for constructing dual recognition site nanopores

The invention discloses a method for constructing a double-recognition site nanopore, which includes: performing structural domain function analysis on two kinds of single-recognition site nanopores fused and expressed to obtain a structural domain analysis result; selecting a structural domain gene and performing a fusion protein Design and obtain a fusion protein with two recognition sites; select an expression system that ensures the stability and polymerizability of the fusion protein structure, and complete the expression scheme design, expression condition optimization, porin polymerization, purification and separation in sequence according to the host type of the expression system , pore embedding and sequencing, and fusion protein mutation processing; this application uses the naturally occurring nanopore protein with a single recognition site, and obtains a nanopore protein with dual recognition sites through engineered fusion protein expression technology, which greatly expands the scope of nanopores. The selectable range of porins solves the problem of few naturally occurring protein nanopores with dual sensitive sites. By mutating the performance of the optimized channel, the detection performance of the channel is improved.
Owner:SHENZHEN MERRIME NANOPORE TECH CO LTD
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