A kind of mnep monomer variant and its application

A variant and monomer technology, applied in the field of characterization of target polynucleotides, can solve the problems of few types of porins, the application of Mnep monomer variant is not mentioned, etc., and achieve the effect of high sequencing accuracy
CN111164097BActive Publication Date: 2021-02-19QITAN TECH LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
QITAN TECH LTD
Publication Date
2021-02-19

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Abstract

The present invention provides a Mnep monomer variant comprising an amino acid sequence of any one or more mutated amino acids at positions 92-104 of SEQ ID NO: 1, and a porin or construct comprising at least one Mnep monomer variant, and its use. The present invention also provides a method of characterizing a target polynucleotide.
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Description

technical field

[0001] The present invention relates to the technical field of characterization of nucleic acid properties, in particular to a Mnep monomer variant, a porin and a construct comprising the Mnep monomer variant, and the application of the Mnep monomer variant or porin to characterize a target polynucleoside sour method. Background technique

[0002] Nanopore sequencing technology is a kind of single-stranded nucleic acid molecule as a sequencing unit, using a nanopore that can provide an ion current channel, so that the single-stranded nucleic acid molecule passes through the nanopore driven by an electric field force, when polynucleotides pass through the nanopore easily When the site is in place, a corresponding blocking current will be generated due to the physical occupying effect, and the different signals generated are read in real time and then analyzed to obtain the gene sequencing technology of the polynucleotide sequence information. Nanopore sequenc...

Claims

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