Mutant of pore protein monomer, protein pore and application thereof

Abstract

The invention belongs to the technical field of characterization of target analyte characteristics, and particularly provides a mutant of a pore protein monomer, a protein pore containing the mutant and application of the mutant to detection of a target analyte. The amino acid of the mutant of the pore protein monomer comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity with the sequence as shown in SEQ ID NO:1, and the amino acid of the mutant of the pore protein monomer comprises mutations at one or more positions corresponding to Q68, N72 and F73 of SEQ ID NO:1.

Description

technical field

[0001] The invention belongs to the technical field of characterization of target analyte characteristics, and particularly relates to a mutant of a porin monomer, a protein pore containing the same and its application for detecting target analytes. Background technique

[0002] With the research on the structure and sequence of nucleic acid, nucleic acid sequencing technology continues to develop, becoming the core field of life science research, and playing a huge role in promoting the development of technology in the fields of biology, chemistry, electricity, life science, and medicine. Using nanopores to develop a new type of rapid, accurate, low-cost, high-precision and high-throughput nucleic acid sequencing technology is one of the hot spots of the post-human genome project.

[0003] Nanopore sequencing technology, also known as the fourth-generation sequencing technology, is a single-stranded nucleic acid molecule as a sequencing unit, using a nanopor...

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