Method for improving stability of nano material
A technology of nanomaterials and stability, which is applied in the fields of nanoscience, protein and microbiology, and can solve problems such as long synthesis cycle, high environmental risk, and poor stability of nano-selenium
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Embodiment 1
[0068] Example 1 Separation and characterization of biological nano-selenium
[0069] 1. Synthesis and purification of biological nano-selenium
[0070] The method for synthesizing biological nano-selenium by Lahenia aquatica HX2 refers to Chinese patent CN104774875B. Bio-nano selenium with particle sizes of 260, 146, 118, 90 and 52 nm was prepared from the prepared biological nano-selenium by density gradient centrifugation. The particle size, particle size distribution and polydispersity index of nano-selenium were determined by dynamic light scattering method, and the results are shown in Table 1. The particle morphology was observed by transmission electron microscopy, such as figure 1 As shown in A-E, the bio-nano selenium with different particle sizes are spherical, and the particle size observed by transmission electron microscope is consistent with the results determined by dynamic light scattering method.
[0071] Table 1
[0072]
[0073] 2. Preparation of che...
Embodiment 2
[0080] Example 2 HX2 strain synthetic biological nano selenium has strong stability
[0081] 1. The stability of nano-selenium synthesized by HX2 under salt ions
[0082] Final concentration of metal ion salt solution: 1mM, 10mM, 100mM, 1000mM NaCl, 0.1mM, 1mM, 10mM, 100mM MgCl 2 , 0.1mM, 1mM, 10mM, 100mM CaCl 2 , 0.001mM, 0.01mM, 0.1mM, 1mM, 10mM, 100mM AlCl 3 . Take a 96-well plate and add 100 μL of NaCl and MgCl of the corresponding concentrations respectively. 2 , CaCl 2 , AlCl 3 ; Add 100 μL of 2mM biological and chemically synthesized nano-selenium solution to the corresponding salt solution wells, each treatment was repeated three times, and treated at 25 °C for 1 h; use a full-wavelength scanner to read the extinction value at 308 nm wavelength; Calculate the control sample and treatment The ratio of the extinction value of the sample at 308nm wavelength was used to quantify the aggregation degree of chemically synthesized nano-selenium and biological nano-seleni...
Embodiment 3
[0109] Example 3 Stripping and mass spectrometry identification of surface-specific proteins of biological nano-selenium
[0110] 1. Stripping of biological nano-selenium-coated proteins
[0111] Urea treatment strips biological nano-selenium-coated proteins. Take 40 mg of purified bio-nano selenium, treat the bio-nano selenium sample with 7M urea solution, 100 ℃ water bath for 5 min, centrifuge at 4 ℃ for 20 min after treatment, take the centrifuged supernatant, and use polyacrylamide gel electrophoresis (SDS-PAGE) to detect the protein peeling effect. The result is as Image 6 As shown, two protein bands could be separated from the purified bio-nano-Se surface after treatment with 7M urea.
[0112] SDS treatment strips the bio-nano-selenium-coated proteins. Take 40 mg of purified bio-nano-selenium, and treat the bio-nano-selenium samples with 4% SDS urea solution in a water bath at 100 °C for 5 min. After treatment, centrifuge at 4 °C for 20 min. Detection of protein st...
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