A novel coronavirus nucleic acid detection kit

A detection kit, coronavirus technology, which is applied in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., can solve the problems of the detection kit sensitivity and specificity need to be improved, and the research and development cycle is short. , to achieve the effect of real-time observation of data changes, avoiding contamination of samples, and reducing contact

Active Publication Date: 2022-07-08
HANGZHOU ANYU TECH CO LTD
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AI Technical Summary

Problems solved by technology

The current novel coronavirus antibody detection kit also needs to be improved due to the short development cycle, and the sensitivity and specificity of the detection kit

Method used

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  • A novel coronavirus nucleic acid detection kit
  • A novel coronavirus nucleic acid detection kit
  • A novel coronavirus nucleic acid detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Development of new coronavirus nucleic acid detection kit

[0041] 1. Design of primers

[0042]Referring to the new coronavirus diagnosis and treatment guidelines, in order to ensure the detection of the new coronavirus and avoid false positives or false negatives, the conserved regions of the new coronavirus ORF1ab gene (Gene ID: 43740578) and S gene (Gene ID: 43740568) were selected as targets point to check.

[0043] Design primers according to the following primer design requirements with reference to the general technical regulations for cross-primer isothermal amplification detection methods:

[0044] (1) The distance between the stripping primers is between 150 and 200 bp, the length of the stripping primers is between 17 and 20 bp, and the temperature is between 48 and 58 °C;

[0045] (2) The distance between the cross-amplification primers is between 45 and 100 bp, the length of the cross-amplification primer is between 34 and 42 bp, and the temperature of a...

Embodiment 2

[0058] 1. Design of primers

[0059] Same as Example 1.

[0060] 2. Composition of the new coronavirus nucleic acid detection kit

[0061] The composition and concentration of the new coronavirus nucleic acid detection kit are shown in Table 4 and Table 5, respectively.

[0062] Table 4 Composition of the new coronavirus nucleic acid detection kit

[0063] component name Volume (μL) Element first container The reaction solution 800 dNTPs, 10×buffer, cyclohexanol succinate second container Detection liquid 50 Primers, SYBR Green I third container enzyme solution 200 Reverse transcriptase, Bst DNA polymerase, ALV reverse transcriptase, Rnase inhibitor fourth container MgCl 2

[0064] Table 5 Concentrations of components of the new coronavirus nucleic acid detection kit

[0065] Element concentration dATP 500nM dTTP 500nM dCTP 500nM dGTP 500nM Cyclohexanol succinate 0.5μg / mL ...

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Abstract

The invention discloses a novel coronavirus nucleic acid detection kit, which belongs to the technical field of biological detection. The present invention uses the real-time fluorescence quantitative cross primer isothermal amplification technology to detect the novel coronavirus, and designs two sets of primers suitable for this technology. The two sets of primers are respectively for the detection of the ORF1ab gene in the type coronavirus genome and the S gene in the type coronavirus genome. The novel coronavirus nucleic acid detection kit of the invention has strong specificity and high sensitivity, not only can qualitatively and quantitatively detect samples, but also can observe changes in data in real time, which is conducive to the comprehensive collection of sample data and facilitates further research. Therefore, the present invention is not only suitable for on-site rapid detection, but also for detection for the purpose of scientific research.

Description

technical field [0001] The present invention relates to the technical field of biological detection, in particular to a novel coronavirus nucleic acid detection kit. Background technique [0002] Coronavirus (CoV) is a class of RNA viruses with enveloped genomes and a linear single-stranded positive-stranded genome. Coronaviruses are divided into 4 genera, alpha, beta, gamma and delta, among which alpha and beta coronaviruses can infect mammals, while gamma and delta coronaviruses mainly infect birds. Six coronaviruses have previously been found to infect humans: alphacoronavirus (HCoV-229E, HCoV-NL63) and betacoronavirus (HCoV-HKU1, HCoV-OC43, MERS-CoV, SARS-CoV). Cold to severe lung infection. Novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) is a beta-coronavirus that has not been found in humans before. Respiratory secretions and direct contact transmission. Patients with COVID-19 often present with specific similar symptoms, such as fev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11
CPCC12Q1/701C12Q1/6844C12Q2600/166C12Q2531/119C12Q2537/1376C12Q2563/107C12Q2527/127C12Q2521/107C12Q2545/113Y02A50/30
Inventor 冯瑞娟章贤骏李海锋陈倩倩
Owner HANGZHOU ANYU TECH CO LTD
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