A tissue-guided regeneration collagen film and its preparation method

A technology that guides regeneration and collagen membranes, applied in surgery, medical science, etc., can solve problems such as unsuitable pore size and porosity, incomplete removal of immunogens, immune reactions, etc., and achieve long degradation cycle, excellent tissue repair effect, comprehensive effect comprehensive effect

Active Publication Date: 2022-04-12
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing technologies include: preparing animal dermis and pericardium through decellularization technology to guide tissue regeneration membrane materials, but this technology has the problem of incomplete removal of immunogens and postoperative immune reactions; another technology is through the method of purification Collagen is extracted from animal connective tissue, and then prepared by cross-linking and freeze-drying technology to guide tissue regeneration collagen film, but this technology has poor mechanical strength, short degradation cycle, pore size and porosity are not suitable for cell growth, resulting in poor repair effect. question

Method used

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  • A tissue-guided regeneration collagen film and its preparation method
  • A tissue-guided regeneration collagen film and its preparation method
  • A tissue-guided regeneration collagen film and its preparation method

Examples

Experimental program
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Embodiment 1

[0025] This embodiment provides a method for preparing a tissue-guided regeneration collagen membrane, comprising the following steps:

[0026] (1) Swell type I collagen with a purity of 90% in an acetic acid solution with a concentration of 0.4% (v / v), and prepare it to obtain a collagen-acetic acid swelling solution with a concentration of 0.3% (w / v) Add chondroitin sulfate sodium and stir evenly, the concentration of chondroitin sulfate sodium is 0.02% (w / v), and after completion, collagen-chondroitin sulfate sodium slurry is obtained;

[0027] (2) Pour the collagen-sodium chondroitin sulfate slurry into a stainless steel freeze-drying tray to make a thickness of 1 mm, and then place the freeze-drying tray in a vacuum freeze dryer for vacuum freeze-drying to obtain a dried membrane;

[0028] (3) Mix the dry membrane with a cross-linking solution with a concentration of 0.01% (v / v), and the ratio of the area of ​​the dry membrane to the volume of the cross-linking solution i...

Embodiment 2

[0031] This embodiment provides a method for preparing a tissue-guided regeneration collagen membrane, comprising the following steps:

[0032] (1) Swell type I collagen with a purity of 92% in an acetic acid solution with a concentration of 0.7% (v / v), and prepare it to obtain a collagen-acetic acid swelling solution with a concentration of 1.0% (w / v) Add chondroitin sulfate sodium and stir evenly, the concentration of chondroitin sulfate sodium is 0.05% (w / v), and after completion, collagen-chondroitin sulfate sodium slurry is obtained;

[0033] (2) Pour the collagen-chondroitin sulfate sodium slurry into a stainless steel freeze-drying tray to make a thickness of 10 mm, and then place the freeze-drying tray in a vacuum freeze dryer for vacuum freeze-drying to obtain a dried membrane ;

[0034] (3) Mix the dry membrane with a cross-linking solution with a concentration of 1% (v / v), and the ratio of the area of ​​the dry membrane to the volume of the cross-linking solution i...

Embodiment 3

[0037] This embodiment provides a method for preparing a tissue-guided regeneration collagen membrane, comprising the following steps:

[0038] (1) Swell type I collagen with a purity of 91% in an acetic acid solution with a concentration of 0.5% (v / v), and prepare it to obtain a collagen-acetic acid swelling solution with a concentration of 0.6% (w / v) Add chondroitin sulfate sodium and stir evenly, the concentration of chondroitin sulfate sodium is 0.03% (w / v), and after completion, collagen-chondroitin sulfate sodium slurry is obtained;

[0039] (2) Pour the collagen-chondroitin sulfate sodium slurry into a stainless steel freeze-drying tray to make the thickness 5mm, then place the freeze-drying tray in a vacuum freeze dryer for vacuum freeze-drying to obtain a dried membrane;

[0040] (3) Mix the dry membrane with the cross-linking solution with a concentration of 0.5% (v / v), and the ratio of the area of ​​the dry membrane to the volume of the cross-linking solution is 2.5...

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Abstract

The invention relates to a tissue-guided regeneration collagen film and a preparation method thereof. The preparation method is as follows: swell type I collagen in an acetic acid solution, then add sodium chondroitin sulfate and stir evenly to obtain a collagen-chondroitin sulfate sodium slurry; dry the collagen-chondroitin sulfate sodium slurry, Obtain a dry film; mix the dry film with the cross-linking liquid, carry out the cross-linking reaction, and obtain a cross-linked collagen film after completion; clean the cross-linked collagen film and perform secondary drying to complete Then repress it. In the preparation method, high-concentration type I collagen is first swelled in acetic acid solution, and sodium chondroitin sulfate is added to stir, followed by drying, cross-linking, and secondary drying, and the obtained tissue-guided regeneration collagen film has excellent mechanical properties , and the pore size and porosity are conducive to cell growth and repair; in addition, its degradation cycle is long and it is not easy to cause allergies. The comprehensive effect is comprehensive and the applicability is wide.

Description

technical field [0001] The invention belongs to the technical field of regenerative medical materials, and in particular relates to a tissue-guided regeneration collagen film and a preparation method thereof. Background technique [0002] Tooth loss caused by oral diseases such as periodontitis and dental caries has become a common oral disease. According to the survey, more than half of our population has tooth loss. With the continuous development of science, dental implant technology has become a common method for the restoration of tooth loss. However, clinically, due to the physiological bone resorption after tooth loss and the bone tissue defect in traumatic tooth loss, some alveolar ridges are often too low. , Too narrow or partially defective cases, 40% to 80% of the patients have insufficient bone mass. If not treated, lateral perforation often occurs during the implantation process, resulting in implant failure. With the clinical application of guided regeneration...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/28C08J9/36C08L89/00C08L5/08A61L31/04A61L31/14
CPCC08J9/28C08J9/36A61L31/148A61L31/041A61L31/146C08J2389/00C08J2405/08
Inventor 黄果张伟孙冰冰
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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