37 results about "Chondroitin sulfate sodium" patented technology

The invention discloses an enzymatic method for preparing chondroitin sulfate and belongs to the medical field. Chondroitin is used as a substrate, chondroitin sulfate A with bioactivity is formed through catalysis of chondroitin 4-sulfate transferase for microbial cell heterologous expression or chondroitin sulfate C with bioactivity is formed through catalysis of chondroitin 6-sulfate transferase, and a PAPS sulfuric donor and a regeneration system are provided by acyl sulfate transferase. A new way is provided for synthesis and industrial production of biological active substances, namely, chondroitin sulfate A and chondroitin sulfate C.

A process for improving quality and yield of collagen peptide coproduced from sodium chondroitin sulfate comprises the following steps: (1) degreasing bovine cartilage by supercritical/subcritical extraction; (2) hydrolyzing, flocculating and separating endonuclease; (3) carrying out ultrafiltration to separate chondroitin sulfate sodium and collagen peptide; (4) refining the sodium chondroitin sulfate; and (5) refining the collagen peptide. The process is used for extracting the dried bovine cartilage chondroitin sulfate sodium and collagen peptide, meanwhile, the product yield and quality are improved, and the obtained product is white in color and luster and free of beef tallow smell. According to the process, the dried bovine bones are adopted, so that the problems that domestic bovinefeeding and slaughtering are scattered, and fresh bovine cartilage is high in storage, collection and transportation cost and high in difficulty are solved; and the process is simple in technologicalprocess, easy to filter, short in production time, capable of saving energy and reducing emission, low in production cost and remarkable in economic benefit and social benefit, and processes such asresin decoloration and ion exchange are not needed.

Relating to the technical field of health food, the invention provides a glucosamine chondroitin calcium tablet and a preparation method thereof. The glucosamine chondroitin calcium tablet disclosed by the invention is composed of the following raw materials: 100-200 weight parts of glucosamine sodium sulfate, 100-200 weight parts of sodium chondroitin sulfate, 80-140 weight parts of calcium carbonate, 150-180 weight parts of collagen peptide, 140-180 weight parts of a herba epimedii extract and 200-350 weight parts of an auxiliary material. The cooperative use of sorbitan monostearate and microcrystalline cellulose improves the cohesion among particles, and enhances the hardness of the glucosamine chondroitin calcium tablet, so that the prepared glucosamine chondroitin calcium tablet doesnot easily absorb external moisture, is not easily damped and is not oxidized easily, therefore the improving effect of the calcium tablet on osteoarthritis is stable.

The invention discloses a method of extracting chondroitin 6-sulfate sodium salt from animal cartilage by solid-phase alkali hydrolysis; the method replaces existing common liquid-phase alkali-enzymatic hydrolysis by solid-phase alkali hydrolysis; at room temperature, animal cartilage powder is mixed with NaOH and NaCl, reaction is triggered under the action of mechanical force, and the extraction and separation process of chondroitin 6-sulfate sodium salt in solid phase is finished efficiently by making use of the features of the solid phase reaction, such as high selectivity, high yield and good process simplicity; the method has the advantages of good process simplicity, high extraction rate, short production cycle, low solvent usage, and low emission of liquid waste.

The invention relates to the industries of functional food and medicine, in particular to a glucosamine chondroitin sulfate salt as well as a preparation method and application thereof. The structuralformula of the glucosamine chondroitin sulfate salt is shown as a formula (1), and the value range of the average polymerization degree n is 5-1000. The glucosamine chondroitin sulfate salt is obtained by performing ion exchange on glucosamine hydrochloride or sulfate or phosphate and chondroitin sulfate sodium, or dialyzing with a mixed solution, or directly precipitating with the mixed solution, , the molecular weights of chondroitin sulfates are all polysaccharides which are obtained through natural extraction, fermentation preparation or degradation, or a synthesis method and accord withthe chondroitin sulfate structures. The chondroitin sulfate and glucosamine are organically combined together, the glucosamine chondroitin sulfate salt is different from an existing compound product of the chondroitin sulfate and the glucosamine, the glucosamine chondroitin sulfate salt does not contain micromolecular inorganic salts or is very low in content of the micromolecular inorganic salts,and the prepared glucosamine chondroitin sulfate salt has a good treatment effect on osteoarthritis model rats in an oral gavage mode; the micromolecular inorganic salts be used for but not limited to joint treatment or auxiliary health care. The glucosamine chondroitin sulfate salt can be widely applied to the fields of functional foods and medicines.

The invention relates to the technical field of biological medicines, in particular to a sinomenine-loaded nano-drug complex and a preparation method thereof. The preparation method of the nano-drug complex comprises the following steps: (1) dissolving sinomenine and a nano-drug carrier in an ethanol solution to obtain an intermediate solution; and (2) mixing the intermediate solution with a chondroitin sulfate C sodium salt aqueous solution according to a volume ratio of 1: (9-11), and adjusting the pH value to obtain the nano-drug complex. The nano-drug complex prepared according to the steps is good in compatibility, stable, biodegradable and small in toxic and side effects.

After a solution obtained by extracting protein from cartilages of fishes which are enzyme treated is filtered, alcohol is added to a filtrate to deposit sodium chondroitin sulfate. The sodium chondroitin sulfate manufactured thus can be used for a raw material of medicines, medicine derivatives, medicine additives, cosmetics, and food additives. After a solution obtained by extracting protein which is obtained by enzyme treatment of cartilages of fishes is filtered, the filtrate is dried to manufacture a chondroitin-sulfuric-acid-containing substance. The chondroitin-sulfuric-acid-containing substance manufactured thus is used for, for instance, cosmetics, and food materials.

The invention relates to a method for increasing the content of chondroitin sulfate sodium. Chondroitin sulfate sodium is a mucopolysaccharide substance extracted from animal cartilages such as nasal bones of pigs and the like. The content of the chondroitin sulfate sodium product prepared by a traditional process is 90-95%, and the limit of protein impurities is above 5% while the European Pharmacopoeia stipulates that the content of chondroitin sulfate sodium is not lower than 95% and the limit of protein impurities is not higher than 3%. Visibly, the traditional process can not meet the requirements of the European Pharmacopoeia. In the method, the protein impurities in the chondroitin sulfate sodium product are removed by adopting a method of thermal precipitation. The process is reasonable, is high in removal efficiency, improves the product content and meets the requirements of the European Pharmacopoeia.

The present invention relates to the technical field of food and particularly discloses a formula food for enhancing bone density of joints and a preparation method thereof. The formula food comprisesthe following raw materials in parts by weight: 15-25 parts of chondroitin sulfate, 10-15 parts of D-glucosamine hydrochloride, 5-10 parts of a rhizoma drynariae extract, 4-6 parts of collagen and 5-10 parts of vitamin C. By adding the chondroitin sulfate, the formula food for enhancing the bone density of the joints can stimulate chondrocyte to synthesize collagen, proteoglycan and HA and control formation of new cartilage tissues. A combination of chondroitin sulfate sodium salt, the D-glucosamine hydrochloride and collagen can treat arthritis and relieve symptoms, and the chondroitin sulfate can remove a large amount of Na+ in synovial membranes, promote bone growth and restore damaged cartilages to normal, and by adding the rhizoma drynariae extract, the whole formula food is rich inflavonoids, alkaloids, phenols and other effective ingredients, and has effects of dispersing stasis, stopping pains and setting bones and tendons.

The invention provides a glucosamine chondroitin tablet containing active peptide and organic calcium and a preparation method of the glucosamine chondroitin tablet. The glucosamine chondroitin tablet comprises 50 to 250 parts of active peptide, 180 to 550 parts of calcium citrate malate, 80 to 520 parts of glucosamine potassium sulfate, 110 to 290 parts of chondroitin sulfate sodium, 10 to 90 parts of diluent, 30 to 170 parts of dry adhesive, 1 to 20 parts of adsorption hardening agent, 1 to 14 parts of lubricant, 0.05 to 3 parts of anti-sticking agent, 1 to 22 parts of film-forming agent, 0.01 to 7 parts of plasticizer and 0.05 to 5 parts of screening agent. The glucosamine chondroitin tablet disclosed by the invention is good in stability and high in calcium ion human body absorption and utilization rate, and can provide a safe and effective calcium source for patients with osteoporosis; meanwhile, damage to the tabletting mold in the production process is effectively reduced, the production and maintenance cost is reduced, dust on one side during tabletting is reduced, the product yield is increased, and the tabletting device is suitable for industrial large-scale production.

The invention discloses a method for purifying chondroitin sulfate sodium salt. The purification method comprises the following steps: (1) dissolving a crude chondroitin sulfate sodium salt in water, adding the crude chondroitin sulfate sodium salt onto a strong acidic cation-exchange resin column, collecting the column liquid, eluting by using purified water, and collecting the eluent for later use; and (2) adding the collected eluent in the step (1) onto a macroporous faintly alkaline cation exchange column, sequentially eluting by using purified water and a sodium chloride solution, collecting the eluent, filtering the eluent, performing ultrafiltration desalting, precipitating by using ethanol, and drying, thereby obtaining the purified chondroitin sulfate sodium salt. The purity of the chondroitin sulfate sodium salt purified by the method disclosed by the invention is over 98.0 percent, the amount of impurities is small, and the protein content can be reduced to be 1.1 percent or below. According to purification in the invention, 100kg of high-purity chondroitin sulfate sodium salt can be obtained per day by virtue of 1000L of resins, and large-scale production can be realized.

The invention discloses a novel method for extracting chondroitin sulfate sodium, namely an alkali-enzymolysis method for extracting chondroitin sulfate sodium, and mainly solves the problems of complicated production technology processes, larger alkali, acid or salt dosage, higher production cost, longer extraction period, unstable product quality, lower yield and the like for similar products at present. The product mainly adopts animal cartilage as a raw material, and chondroitin sulfate sodium can be extracted from the animal cartilage after alkaline hydrolysis and further enzymolysis; and the color of the product is pure white, the powder is loosened, the yield can be higher than 30%, moreover, the purity is high, and the product meets the oral quality standard. The alkali-enzymolysis method for extracting chondroitin sulfate sodium has the characteristics of simple production process, short period, low cost, simplicity, convenience and easiness in operation, capacities of reducing environment pollution and facilitating environmental protection, and the like.

The invention discloses a preparation method of chondroitin sulfate sodium. The method comprises the following steps: adding water into a cartilage raw material, cooking, taking out, and pulverizing to obtain cartilage powder; adding mixed powder of sodium hydroxide and silicon dioxide into the cartilage powder, co-grinding, adding a sodium chloride solution, adjusting the pH value, centrifuging and collecting supernatant; carrying out alkali extraction on the precipitate, centrifuging, and collecting the supernatant; mixing the supernatant, adjusting the pH value to 8-10, and then adding alkaline protease for enzymolysis to obtain a chondroitin sulfate solution; adding an adsorbent, keeping standing, carrying out filter pressing, and collecting a filtrate; and adding ethanol with different concentrations into the filtrate by stages for purification to obtain the product. The method can be used to effectively solve the problems of waste, environmental pollution, long extraction time and low product purity caused by a large amount of sodium hydroxide solution in the existing method.

The invention discloses a compound brain protein hydrolysate tablet and a preparation process thereof, and relates to the technical field of medicine processing. The compound brain protein hydrolysatetablet is prepared from the following components in parts by mass: 60-75 parts of brain protein hydrolysate, 6-7 parts of glutamic acid, 6-7 parts of sodium chondroitin sulfate, 11-13 parts of microcrystalline fibers, 10-12 parts of vitamin B1, 0.1-0.3 part of vitamin B6, 7-9 parts of dextrin, 8-10 parts of calcium carbonate, 10-12 parts of corn starch, 3-5 parts of magnesium stearate for coating, 100-130 parts of sucrose and 60-70 parts of talcum powder. The preparation process comprises the following steps: sequentially granulating the raw materials, drying, granulating and totally mixing,tabletting, coating, and airing, thereby obtaining the compound brain protein hydrolysate tablet. The coating is performed in three steps, namely an isolation layer, a powder coating layer and a colored sugar coating layer. The coating raw materials are prepared from syrup, so that the disintegration time of the coating is accelerated, the drug effect time is prolonged, and the taste of the compound brain protein hydrolysate is guaranteed.

The present invention belongs to the field of health-care food and discloses a composition and a health-care product for strengthening bones and supplementing calcium, and a preparation method of thehealth-care product. The composition for strengthening bones and supplementing calcium comprises the following components: calcium carbonate, glucosamine potassium sulfate, a pseudo-ginseng extract, chondroitin sulfate sodium, casein phosphopeptides and a rhizoma drynariae extract. The composition has good safety and also good effects of strengthening the bones and supplementing calcium.

The invention relates to a preparation method for a selenium-rich aquatic animal nutrient. The formula (in percentage by weight) consists of 0.8 to 1.8 percent of aspartic acid, 0.9 to 1.9 percent ofdl-Alpha-tocopheryl acetate, 1.2 to 2.2 percent of calcium gluconate, 1.5 to 2.5 percent of bifidobacterium longum, 0.2 to 1.2 percent of ammonium chloride, 1 to 2 percent of sea cucumber extract, 2.5to 3.6 percent of lactobacillus, 2.3 to 3.3 percent of sorbitan monooleate, 2.6 to 3.6 percent of inonotus obliquus extract, 2.2 to 3.2 percent of proteus hauser, 1.7 to 2.7 percent of sodium butyrate, 1.9 to 2.8 percent of protease, 0.3 to 1.3 percent of chondroitin sulfate sodium salt, 0.1 to 1.1 percent of ammonium sulfite, 0.6 to 1.6 percent of sodium carboxymethylcellulose, 0.7 to 1.5 percent of vitamin C, 0.5 to 1.6 percent of potassium sulfate, 1.2 to 2.1 percent of trehalose, 1.1 to 2.2 percent of probiotics, 0.2 to 1.3 percent of selenium yeast and 76.5 to 56.5 percent of water. Theinvention can effectively enable the growth performance of aquatic animals to reach an optimal level, and the specific growth rate, the weight gain rate, the feed utilization rate, the protein utilization rate and the feed protein deposition rate can all reach the maximum.