Method for purifying chondroitin sulfate sodium salt

Abstract

The invention discloses a method for purifying chondroitin sulfate sodium salt. The purification method comprises the following steps: (1) dissolving a crude chondroitin sulfate sodium salt in water, adding the crude chondroitin sulfate sodium salt onto a strong acidic cation-exchange resin column, collecting the column liquid, eluting by using purified water, and collecting the eluent for later use; and (2) adding the collected eluent in the step (1) onto a macroporous faintly alkaline cation exchange column, sequentially eluting by using purified water and a sodium chloride solution, collecting the eluent, filtering the eluent, performing ultrafiltration desalting, precipitating by using ethanol, and drying, thereby obtaining the purified chondroitin sulfate sodium salt. The purity of the chondroitin sulfate sodium salt purified by the method disclosed by the invention is over 98.0 percent, the amount of impurities is small, and the protein content can be reduced to be 1.1 percent or below. According to purification in the invention, 100kg of high-purity chondroitin sulfate sodium salt can be obtained per day by virtue of 1000L of resins, and large-scale production can be realized.

Description

technical field [0001] The invention relates to a method for purifying acidic mucopolysaccharides, in particular to a method for purifying crude chondroitin sulfate sodium salt extracted from animal cartilage, and belongs to the field of biochemical pharmacy. Background technique [0002] Chondroitin sulfate sodium salt is an acidic mucopolysaccharide extracted from animal cartilage. It is mainly used to prevent and treat cardiovascular and cerebrovascular diseases. It is also used to prevent deafness caused by streptomycin in my country. [0003] The chondroitin sulfate on the market generally has two specifications, namely 90% and 95% respectively. Both of them have high protein content. It may be that the product user has not taken it and passed the shelf life. [0004] The current production process of chondroitin sulfate sodium salt is generally to dilute animal cartilage first, and then through enzymatic hydrolysis, ethanol precipitation and other steps to produce f...

AI Technical Summary

Problems solved by technology

[0004] The current production process of chondroitin sulfate sodium salt is generally to dilute animal cartilage first, and then through enzymatic hydrolysis, ethanol precipitation and other steps to produce food-grade products with a content of about 90%. Some methods also use ultrafiltration or calcium salt conversion. method to produce medical-grade products with a content greater than 95%, but various existing methods cannot achieve mass production of chondroitin sulfate sodium salt with a protein content greater than 98% and a protein content of less than 1.1%.

There are some reports in the literature that a product with the same low protein content can be trial-produced by using a strong basic anion exchange resin, but it is limited to a laboratory-scale, batch-capable purification method for chondroitin sulfate