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Nano-drug complex loaded with sinomenine and preparation method of nano-drug complex

A nano-drug carrier and nano-drug technology, applied in the field of biomedicine, achieves good biocompatibility, strong operability, and the effect of solving biological toxicity and in vivo metabolism

Pending Publication Date: 2021-11-26
JINING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most drug carriers are inert and only serve as a vehicle for drug delivery

Method used

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  • Nano-drug complex loaded with sinomenine and preparation method of nano-drug complex
  • Nano-drug complex loaded with sinomenine and preparation method of nano-drug complex
  • Nano-drug complex loaded with sinomenine and preparation method of nano-drug complex

Examples

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preparation example Construction

[0039] The invention provides a preparation method of a nano-drug complex loaded with sinomenine, comprising the following steps:

[0040] (1) Dissolving the sinomenine and the nano drug carrier in the ethanol solution to obtain the intermediate solution;

[0041] (2) The intermediate solution is mixed with the chondroitin sulfate C sodium salt solution at a volume ratio of 1:9-11, preferably 1:10, and the pH is adjusted to obtain a nano drug complex.

[0042] In the present invention, the mass ratio of sinomenine to nano drug carrier is 1:5-40, preferably 1:13-32, more preferably 1:21-24, more preferably 1:22.5;

[0043] The nano drug carrier is zein.

[0044] In the present invention, the volume concentration of the ethanol solution is 60-95%, preferably 70-85%, more preferably 80%.

[0045] In the present invention, the mass concentration of the chondroitin sulfate C sodium salt solution is 0.1-0.2%, preferably 0.15%.

[0046] In the present invention, the pH is 6.9-7.1,...

Embodiment 1

[0066] Take by weighing 0.75g zein, 0.0375g sinomenine and dissolve in 30mL70% ethanol solution to obtain an intermediate solution, such as figure 1 shown. According to the volume ratio of 1:10, the intermediate solution was mixed with chondroitin sulfate C sodium salt solution with a concentration of 0.2%. After adjusting the pH to 7 with 0.1M HCl, the Sonicate for 2 minutes at a power of 600W to obtain an ultrasonic solution. Rotate the ultrasonic solution on a rotary evaporator at 40°C for 10 minutes to remove ethanol, and centrifuge the ethanol-removed solution at 6000 rpm for 10 minutes to remove impurities to obtain the nanomedicine complexes of Example 1 under different ultrasonic powers. The influence of different ultrasonic powers on the solution state of the nano drug complex was measured, and the results were as follows: figure 2 shown. Determination of the influence of different ultrasonic powers on the Zeta potential value and particle size of the nano drug comp...

Embodiment 2

[0077] 0.75 g of zein and 0.075 g of sinomenine were weighed and dissolved in 30 mL of 60% ethanol solution to obtain an intermediate solution. Mix the intermediate solution with 0.2% chondroitin sulfate C sodium salt solution at a volume ratio of 1:9, adjust the pH to 7 with 0.05M HCl, and then sonicate for 1.8min at a power of 400W to obtain an ultrasonic solution. Rotate the ultrasonic solution on a rotary evaporator at 38°C for 8 minutes to remove ethanol, and centrifuge the ethanol-removed solution at 6200 rpm for 8 minutes to remove impurities to obtain the nano drug complex of Example 2.

[0078] The preparation method of the 0.2% chondroitin sulfate C sodium salt solution is as shown in Example 1.

[0079] Under the condition of ultrasonic power of 400W, it will be determined that the nano drug complex has a particle size of 241.5±10.4nm, a PDI=0.17±0.03, a charge of -40.4±1.7mV, and an encapsulation efficiency of 59.3±1.7%.

[0080] When the ultrasonic power is 400W,...

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Abstract

The invention relates to the technical field of biological medicines, in particular to a sinomenine-loaded nano-drug complex and a preparation method thereof. The preparation method of the nano-drug complex comprises the following steps: (1) dissolving sinomenine and a nano-drug carrier in an ethanol solution to obtain an intermediate solution; and (2) mixing the intermediate solution with a chondroitin sulfate C sodium salt aqueous solution according to a volume ratio of 1: (9-11), and adjusting the pH value to obtain the nano-drug complex. The nano-drug complex prepared according to the steps is good in compatibility, stable, biodegradable and small in toxic and side effects.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a nano drug complex loaded with sinomenine and a preparation method thereof. Background technique [0002] Sinomenine (Sinomenime, Sin) is a benzyl tetrahydroisoquinoline alkaloid, derived from the roots and stems of the plant Ivy chinensis, and is insoluble in water. Modern pharmacological studies have shown that Sin has pharmacological effects such as anti-inflammatory, analgesic, hypotensive, and anti-arrhythmic; it is often used clinically to treat rheumatoid arthritis with remarkable curative effect. However, the existing Sin-HCl has disadvantages such as low oral bioavailability, short half-life (T1 / 2=1.49h), fast metabolism, etc., resulting in serious side effects. Based on this, researchers have two main research directions for sinomenine dosage forms. The first one is to modify the structure of sinomenine monomer compounds and synthesize their derivatives. This met...

Claims

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Application Information

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IPC IPC(8): A61K47/64A61K47/61A61K31/485A61P19/02A61P29/00B82Y5/00B82Y30/00
CPCA61K47/64A61K47/61A61K31/485A61P19/02A61P29/00B82Y5/00B82Y30/00
Inventor 牛艳莲崔腾黄路芬吕美凌爱霞李伟王慧云王守信
Owner JINING MEDICAL UNIV
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