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Tissue-guided regenerated collagen membrane and preparation method thereof

A technology that guides regeneration and collagen membranes, applied in medical science, surgery, etc., can solve problems such as unsuitable pore size and porosity, incomplete removal of immunogens, immune reactions, etc., to achieve long degradation cycle, excellent tissue repair effect, comprehensive effect comprehensive effect

Active Publication Date: 2022-02-18
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing technologies include: preparing animal dermis and pericardium through decellularization technology to guide tissue regeneration membrane materials, but this technology has the problem of incomplete removal of immunogens and postoperative immune reactions; another technology is through the method of purification Collagen is extracted from animal connective tissue, and then prepared by cross-linking and freeze-drying technology to guide tissue regeneration collagen film, but this technology has poor mechanical strength, short degradation cycle, pore size and porosity are not suitable for cell growth, resulting in poor repair effect. question

Method used

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  • Tissue-guided regenerated collagen membrane and preparation method thereof
  • Tissue-guided regenerated collagen membrane and preparation method thereof
  • Tissue-guided regenerated collagen membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This embodiment provides a method for preparing a tissue-guided regeneration collagen membrane, comprising the following steps:

[0026] (1) Swell type I collagen with a purity of 90% in an acetic acid solution with a concentration of 0.4% (v / v), and prepare it to obtain a collagen-acetic acid swelling solution with a concentration of 0.3% (w / v) Add chondroitin sulfate sodium and stir evenly, the concentration of chondroitin sulfate sodium is 0.02% (w / v), and after completion, collagen-chondroitin sulfate sodium slurry is obtained;

[0027] (2) Pour the collagen-sodium chondroitin sulfate slurry into a stainless steel freeze-drying tray to make a thickness of 1 mm, and then place the freeze-drying tray in a vacuum freeze dryer for vacuum freeze-drying to obtain a dried membrane;

[0028] (3) Mix the dry membrane with a cross-linking solution with a concentration of 0.01% (v / v), and the ratio of the area of ​​the dry membrane to the volume of the cross-linking solution i...

Embodiment 2

[0031] This embodiment provides a method for preparing a tissue-guided regeneration collagen membrane, comprising the following steps:

[0032] (1) Swell type I collagen with a purity of 92% in an acetic acid solution with a concentration of 0.7% (v / v), and prepare it to obtain a collagen-acetic acid swelling solution with a concentration of 1.0% (w / v) Add chondroitin sulfate sodium and stir evenly, the concentration of chondroitin sulfate sodium is 0.05% (w / v), and after completion, collagen-chondroitin sulfate sodium slurry is obtained;

[0033] (2) Pour the collagen-chondroitin sulfate sodium slurry into a stainless steel freeze-drying tray to make a thickness of 10 mm, and then place the freeze-drying tray in a vacuum freeze dryer for vacuum freeze-drying to obtain a dried membrane ;

[0034] (3) Mix the dry membrane with a cross-linking solution with a concentration of 1% (v / v), and the ratio of the area of ​​the dry membrane to the volume of the cross-linking solution i...

Embodiment 3

[0037] This embodiment provides a method for preparing a tissue-guided regeneration collagen membrane, comprising the following steps:

[0038] (1) Swell type I collagen with a purity of 91% in an acetic acid solution with a concentration of 0.5% (v / v), and prepare it to obtain a collagen-acetic acid swelling solution with a concentration of 0.6% (w / v) Add chondroitin sulfate sodium and stir evenly, the concentration of chondroitin sulfate sodium is 0.03% (w / v), and after completion, collagen-chondroitin sulfate sodium slurry is obtained;

[0039] (2) Pour the collagen-chondroitin sulfate sodium slurry into a stainless steel freeze-drying tray to make the thickness 5mm, then place the freeze-drying tray in a vacuum freeze dryer for vacuum freeze-drying to obtain a dried membrane;

[0040] (3) Mix the dry membrane with the cross-linking solution with a concentration of 0.5% (v / v), and the ratio of the area of ​​the dry membrane to the volume of the cross-linking solution is 2.5...

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Abstract

The invention relates to a tissue-guided regenerated collagen membrane and a preparation method thereof. The preparation method comprises the following steps: swelling type I collagen in an acetic acid solution, adding chondroitin sulfate sodium, and uniformly stirring to obtain collagen-chondroitin sulfate sodium slurry; drying the collagen-chondroitin sulfate sodium slurry to obtain a dried membrane; mixing the dried diaphragm with a crosslinking solution, and carrying out a crosslinking reaction to obtain a crosslinked collagen diaphragm after the crosslinking reaction is completed; and cleaning the cross-linked collagen membrane, carrying out secondary drying, and performing pressing after the secondary drying is completed. The preparation method comprises the following steps: firstly, swelling high-concentration I-type collagen in an acetic acid solution, adding sodium chondroitin sulfate, stirring, and then drying, cross-linking and secondarily drying, so that the obtained tissue-guided regenerated collagen membrane has excellent mechanical properties, and the pore size and porosity are beneficial to cell growth and repair; in addition, the degradation period is long, and sensitization is not prone to occurring; and the comprehensive effect is comprehensive, and the applicability is wide.

Description

technical field [0001] The invention belongs to the technical field of regenerative medical materials, and in particular relates to a tissue-guided regeneration collagen film and a preparation method thereof. Background technique [0002] Tooth loss caused by oral diseases such as periodontitis and dental caries has become a common oral disease. According to the survey, more than half of our population has tooth loss. With the continuous development of science, dental implant technology has become a common method for the restoration of tooth loss. However, clinically, due to the physiological bone resorption after tooth loss and the bone tissue defect in traumatic tooth loss, some alveolar ridges are often too low. , Too narrow or partially defective cases, 40% to 80% of the patients have insufficient bone mass. If not treated, lateral perforation often occurs during the implantation process, resulting in implant failure. With the clinical application of guided regeneration...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08J9/36C08L89/00C08L5/08A61L31/04A61L31/14
CPCC08J9/28C08J9/36A61L31/148A61L31/041A61L31/146C08J2389/00C08J2405/08
Inventor 黄果张伟孙冰冰
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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