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Preparation method of multilayer artificial dura mater

An artificial dura mater and serous technology, applied in the direction of bone implants, etc., to achieve the effect of low temperature, excellent suture performance, and optimized process parameters

Active Publication Date: 2022-04-01
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a simple and industrialized biofilm preparation method specially used for transnasal sphenoidal cranial base reconstruction surgery, so as to solve the problems of the existing dura mater products in terms of anti-leakage performance and resilience performance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a kind of preparation method of multi-layer artificial dura mater, comprising the following steps:

[0031] (1) Prepare a 0.05M acetic acid solution with a mass volume concentration of chondroitin sulfate sodium of 0.03% and a collagen protein mass volume concentration of 0.3%, stir for 2 hours with a homogenizer at 18,000 r / min, vacuum defoam, obtain a slurry, and set aside;

[0032] (2) Pour 3 mm deep slurry into freeze-drying tray a, pour 1 mm deep slurry into freeze-drying tray b, pre-freeze the slurry in freeze-drying tray a at -20°C for 8 hours, and freeze cooling the slurry in the drying tray b to 4°C, then pouring the slurry in the freeze-drying tray b into the freeze-drying tray a, and pre-freezing at -80°C for 4 hours to obtain the pre-frozen slurry;

[0033] (3) Place the pre-frozen slurry in a freeze dryer to freeze-dry to obtain a semi-finished product of layered artificial dura mater; the freeze-drying includes the following...

Embodiment 2

[0042] The present embodiment provides a kind of preparation method of multi-layer artificial dura mater, comprising the following steps:

[0043] (1) Prepare a 0.05M acetic acid solution with a mass volume concentration of chondroitin sulfate sodium of 0.1% and a collagen mass volume concentration of 1.0%, stir for 2 hours with a homogenizer at 15,000 r / min, vacuum defoam, obtain a slurry, and set aside;

[0044] (2) Pour 5 mm deep slurry into freeze-drying tray a, pour 2 mm deep slurry into freeze-drying tray b, first pre-freeze the slurry in freeze-drying tray a at -20°C for 16 hours, and freeze cooling the slurry in the drying tray b to 10°C, then pouring the slurry in the freeze-drying tray b into the freeze-drying tray a, and pre-freezing at -80°C for 8 hours to obtain the pre-frozen slurry;

[0045] (3) Place the pre-frozen slurry in a freeze dryer to freeze-dry to obtain a semi-finished product of layered artificial dura mater; the freeze-drying includes the following ...

Embodiment 3

[0054] The present embodiment provides a kind of preparation method of multi-layer artificial dura mater, comprising the following steps:

[0055] (1) Prepare a 0.05M acetic acid solution with a mass volume concentration of chondroitin sulfate sodium of 0.05% and a collagen protein mass volume concentration of 0.5%, stir for 1 hour with a homogenizer at 20,000 r / min, vacuum defoam, obtain a slurry, and set aside;

[0056] (2) Pour 4 mm deep slurry into freeze-drying tray a, pour 2 mm deep slurry into freeze-drying tray b, first pre-freeze the slurry in freeze-drying tray a at -20 °C for 10 h, and freeze cooling the slurry in the drying tray b to 5°C, then pouring the slurry in the freeze-drying tray b into the freeze-drying tray a, and pre-freezing at -80°C for 6 hours to obtain the pre-frozen slurry;

[0057] (3) Place the pre-frozen slurry in a freeze dryer to freeze-dry to obtain a semi-finished product of layered artificial dura mater; the freeze-drying includes the follow...

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Abstract

The invention relates to a preparation method of a multi-layer artificial dura mater, which comprises the following steps: preparing serous fluid containing chondroitin sulfate sodium and collagen, and sequentially carrying out pre-freezing, freeze drying, high-temperature vacuum crosslinking and sterilization treatment on the serous fluid. According to the method, a special gradient pre-freezing process is adopted, after pre-freezing, the multi-layer artificial dura mater with different densities can be obtained only through one-time freeze drying, and meanwhile process parameters of high-temperature vacuum crosslinking are optimized. The finally prepared multi-layer artificial dura mater is excellent in anti-seepage performance, anti-degradation performance and sewing performance.

Description

technical field [0001] The invention belongs to the technical field of dura mater, and in particular relates to a preparation method of multilayer artificial dura mater. Background technique [0002] The dura mater is a membranous tissue between the skull and the brain tissue, and is an important protective membrane for the brain tissue. Trauma, inflammation, tumor erosion, and surgical operations can all cause damage to the dura mater and destroy its integrity. Therefore, dura mater repair is an important step in cranial surgery, which can better maintain the integrity of dura mater and prevent cerebrospinal fluid leakage. [0003] Among the dura mater products currently used in clinical practice, acellular matrix products are usually made from raw materials such as pig peritoneum, sheep peritoneum, and bovine pericardium through decellularization technology. These materials are structurally similar to the dura mater and help restore normal physiological anatomy. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/28
Inventor 田翔宇张伟张伊骁孙冰冰
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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